ABSTRACT
Morphological, functional signs of myocardium affection and tolerance to physical loading in animals with isoproterenol-induced affection of myocardium after transplantation of a nuclear-containing cells (NCC) of umbilical blood were analyzed in comparison with a natural course of the model. There was proved, that a transplantation of a NCC promotes acceleration of processes of regeneration and restoration of the damaged myocardium in experimental animals. There were estimated the changes in localization of the transplanted NCC in the zone of damage, and capacity of the peripheral blood NCC, while they were transplanted, using intravenous injection, to migrate into the damage zone. The experimental data obtained need further complex analysis and permit to wait a positive effect while performing clinical investigations in the patients, suffering chronic diseases of the heart.
Subject(s)
Cord Blood Stem Cell Transplantation , Fetal Blood/cytology , Myocardial Infarction/therapy , Myocardium/pathology , Myocytes, Cardiac/pathology , Animals , Cryopreservation , Female , Fetal Blood/physiology , Fetal Blood/transplantation , Humans , Isoproterenol , Mice , Myocardial Infarction/chemically induced , Myocardial Infarction/pathology , Regeneration/physiology , Transplantation, HeterologousABSTRACT
AIM: To detect changes in the concentration of nitric oxide and its metabolites--nitrites and nitrates in conjunction with the indices of the glutathione system in the children with chronic hepatitis B and C. PATIENTS AND METHODS: In the research took a part 76 patients (boys or girls) with chronic hepatitis B and C aged from 7 up to 16 years. Nitric oxide and its metabolites determined by method of V.A. Metel'skaya and N.G. Gumanova; level of reduced glutathione - the titrimetric analysis; activity of glutathione transferase - the method of spectrophotometry. RESULTS: The content of nitric oxide and its metabolites--nitrites and nitrates--in serum and urine of children with chronic viral hepatitis B and C have increased. Correlations were revealed between nitric oxide and its metabolites in serum and urine, as well as between these indices and glutathione system parameters-reduced glutathione concentration and activity of enzymes of glutathione transferase of erythrocytes and plasma. CONCLUSIONS: In the children with chronic viral hepatitis B and C the increase of the concentration of nitric oxide and its metabolites in serum and urine is accompanied by decrease of reduced glutathione concentration and increase of glutathione transferase activity in erythrocytes and plasma. These findings suggest the presence of oxidative stress due to which the therapy should be strengthened by antioxidant effect.
Subject(s)
Glutathione/metabolism , Hepatitis B, Chronic/metabolism , Hepatitis C, Chronic/metabolism , Nitric Oxide/metabolism , Oxidative Stress , Adolescent , Biomarkers/blood , Biomarkers/urine , Child , Female , Humans , Male , SpectrophotometryABSTRACT
In the present study liver detoxifying function state in children with chronic hepatitis and pigmentary hepatosis was examined. An endogenous intoxication level increase, peroxide processes and biotransformation system activation were established against the background of endotoxicosis substrate accumulation in blood. The dynamics of clinical symptoms and liver detoxifying function indicators was estimated against the background of the complex treatment with Reamberin. In our study we have noticed detoxication mechanisms improvement, and that fact has been confirmed by a decrease of endogenous intoxication level and significant middle-mass molecules decreasing in blood plasma as well as intoxication index and glutathione enzymes activity in blood.
Subject(s)
Hepatitis, Chronic/drug therapy , Hepatitis, Chronic/pathology , Meglumine/analogs & derivatives , Metabolic Detoxication, Phase II , Succinates/administration & dosage , Adolescent , Child , Child, Preschool , Female , Glutathione Reductase/blood , Glutathione Transferase/blood , Hepatitis, Chronic/virology , Humans , Male , Malondialdehyde/blood , Meglumine/administration & dosageABSTRACT
In the experiments on dogs and mice it was shown that indometophene could modify the immune status of the body. Specific and non-specific resistance of mice to E. coli infection increased after indometophene administration. At the same time higher titers of normal anti-salmonella antibodies in mice serum and a higher phagocytic activity of neutrophils in the peripheral blood in dogs were found. The influence of the preparation on the content of the complement and normal antibodies-hemagglutinins was less pronounced. It was suggested that the indometophene ability to bustle immune reactivity of the body played a certain role in the mechanism of its radioprotective effect.
