ABSTRACT
The authors have got their first experience with chemohyperthermal perfusion of the lung in 4 patients. The method and technique of the operation of ablation of metastases in the lungs with preliminary chemohyperthermal perfusion are described. All the patients survived the operative interventions.
Subject(s)
Chemotherapy, Cancer, Regional Perfusion/methods , Lung Neoplasms/drug therapy , Lung Neoplasms/secondary , Aged , Female , Humans , Lung Neoplasms/surgery , Male , Middle Aged , Neoplasm Recurrence, Local/diagnostic imaging , Radiography , Treatment OutcomeABSTRACT
A preventive short-term hypoxia (preconditioning) increases neuronal resistance against subsequent strong hypoxic effects. Literature review and authors' own data on molecular-cellular mechanisms of the hypoxic preconditioning, are presented. Participation of intracellular signal transduction, genome, stress-proteins, and neuromodulating peptides in this process, is discussed. The role of glutamatergic as well as calcium and phosphoinositide regulatory systems and neuromodulating factors as the components of a "volume" signal transmission are analyzed in hypoxic precondition-associated induction of functional tolerance mechanisms against acute harmful effects in neurones of olfactory slices.
Subject(s)
Adaptation, Physiological , Brain/physiology , Neurons/physiology , Animals , Brain/metabolism , Cell Hypoxia , Genome , Heat-Shock Proteins/metabolism , Neurons/metabolism , Signal TransductionABSTRACT
Heroin addicts at the initial stage of abstinence syndrome were subjected to detoxication by liquorosorption technique. The fractions of their cerebrospinal fluid obtained by the thin layer chromatography technique were analyzed. The substances extracted from the cerebrospinal fluid of drug addicts, presumably peptides, negatively affected the conductive function and synaptic transmission in surviving slices of the olfactory cortex of rats. The conclusion was drawn about a possibility of application of surviving rat brain slices as test object for estimation of the extent of purification of the cerebrospinal fluid from toxic endogenous substances after the liquorosorption.
Subject(s)
Biological Factors/pharmacology , Heroin Dependence/cerebrospinal fluid , Olfactory Pathways/drug effects , Adolescent , Adult , Animals , Biological Factors/cerebrospinal fluid , Chemical Fractionation , Chromatography, Thin Layer , Electrophysiology , Humans , In Vitro Techniques , Male , Olfactory Pathways/physiology , Rats , Rats, Wistar , Synaptic Transmission/drug effectsSubject(s)
Heroin Dependence/cerebrospinal fluid , Action Potentials/drug effects , Action Potentials/physiology , Adolescent , Adult , Animals , Cerebrospinal Fluid/chemistry , Dose-Response Relationship, Drug , Electrophysiology , Heroin Dependence/physiopathology , Humans , In Vitro Techniques , Male , Olfactory Pathways/drug effects , Olfactory Pathways/physiology , Rats , Rats, Wistar , Sorption Detoxification , Time FactorsABSTRACT
The participation of NMDA receptors in the changes caused by evoked EPSPs in the activity of calcium and phophoinositide intracellular regulatory systems (ICRS) bioelectric activity (evoked EPSPs) and in cell production of polypeptides was studied in rat olfactory cortical slices exposed to short-term anoxia (STA) and long-term anoxia (LTA). To determine NMDA receptor involvement in these functional and metabolic reactions, the slices were treated with the antagonists APV and MK-801 during anoxia of different duration (STA and LTA being 2 and 10 min, respectively) and subsequent reoxygenation. EPSPs were recorded extracellularly in response to lateral olfactory tract stimulation. The STA-mediated stimulation of NMDA receptors was found to promote adaptive reactions (moderate activation of IRS, long-term potentiation of synaptic transmission) in the postanoxic period. LTA induced the long-lasting stimulation of NMDA receptors that led to the hyperactivation of calcium and phosphoinositide IRS, to the suppression of generation of EPSPs and of synthesis of polypeptides probably of adaptive action. All these events induced pathological processes in the postanoxic period.
Subject(s)
Hypoxia, Brain/metabolism , Neurons/physiology , Receptors, N-Methyl-D-Aspartate/metabolism , Adaptation, Physiological , Animals , Calcium/metabolism , Excitatory Postsynaptic Potentials/physiology , Hypoxia, Brain/physiopathology , Intracellular Fluid/metabolism , Male , Phosphatidylinositols/metabolism , Rats , Rats, Inbred WKY , Recovery of Function/physiology , Signal Transduction/physiology , Time FactorsABSTRACT
Muscle incubate was shown to contain factors capable to increase the transmitter release in low-effective synapses and decrease it in high-effective those, dueto activation and inhibition, respectively, of the processes governing formation of available store of the transmitter. Activatiory effect of the low-molecular fraction on the low-effective synapses was correlated with the concentration of histidine-containing substances in this fraction.
Subject(s)
Histidine/metabolism , Muscle Proteins/metabolism , Muscle, Skeletal/innervation , Nerve Endings/metabolism , Neurotransmitter Agents/metabolism , Animals , Culture Media , Culture Techniques , Male , Rana temporariaABSTRACT
Histidine-containing compounds (HCC) were found, using diazoreaction and reaction with diethylpyrocarbonate, a specific histidine reagent, in the perfusate of the frog hindlimb vasculature. After gel filtration on sephadex G-25 about 80% of HCC were eluted with a fraction with molecular mass less than 5000 kD. The studies of the perfusate influence on the characteristics of quantum secretion of transmitter in the preparation of frog cutaneopectoral muscle have shown that the perfusate increased the quantum content of end-plate potentials (EPP) due to increasing binominal parameter and decreasing frequency of miniature EPP. The characteristics of presynaptic action of the perfusate were similar to those of exogenous histidine.
