Nucleus-cytoskeleton communication impacts on OCT4-chromatin interactions in embryonic stem cells.

BACKGROUND: The cytoskeleton is a key component of the system responsible for transmitting mechanical cues from the cellular environment to the nucleus, where they trigger downstream responses. This communication is particularly relevant in embryonic stem (ES) cells since forces can regulate cell fate and guide developmental processes. However, little is known regarding cytoskeleton organization in ES cells, and thus, relevant aspects of nuclear-cytoskeletal interactions remain elusive. RESULTS: We explored the three-dimensional distribution of the cytoskeleton in live ES cells and show that these filaments affect the shape of the nucleus. Next, we evaluated if cytoskeletal components indirectly modulate the binding of the pluripotency transcription factor OCT4 to chromatin targets. We show that actin depolymerization triggers OCT4 binding to chromatin sites whereas vimentin disruption produces the opposite effect. In contrast to actin, vimentin contributes to the preservation of OCT4-chromatin interactions and, consequently, may have a pro-stemness role. CONCLUSIONS: Our results suggest roles of components of the cytoskeleton in shaping the nucleus of ES cells, influencing the interactions of the transcription factor OCT4 with the chromatin and potentially affecting pluripotency and cell fate.

Analysis of ORF5 sequences of Porcine Reproductive and Respiratory Syndrome virus (PRRSV) circulating within swine farms in Costa Rica.

BACKGROUND: Worldwide, Porcine Reproductive and Respiratory Syndrome (PRRS) is among the diseases that cause the highest economic impact in modern pig production. PRRS was first detected in Costa Rica in 1996 and has since then severely affected the local swine industry. Studies of the molecular characterization of circulating strains, correlation with clinical records, and associations with pathogens associated with Porcine Respiratory Disease Complex (PRDC) have not been done in Costa Rica. RESULTS: Sequencing and phylogenetic analysis of ORF5 proved that PRRSV-2 was the only species detected in all locations analyzed. These sequences were grouped into three clusters. When comparing samples from San Jose, Alejuela, and Puntarenas to historical isolates of the previously described lineages (1 to 9), it has been shown that these were closely related to each other and belonged to Lineage 5, along with the samples from Heredia. Intriguingly, samples from Cartago clustered in a separate clade, phylogenetically related to Lineage 1. Epitope analysis conducted on the GP5 sequence of field isolates from Costa Rica revealed seven peptides with at least 80% amino acid sequence identity with previously described and experimentally validated immunogenic regions. Previously described epitopes A, B, and C, were detected in the Santa Barbara-Heredia isolate. CONCLUSIONS: Our data suggest that the virus has three distinct origins or introductions to the country. Future studies will elucidate how recently introduced vaccines will shape the evolutionary change of circulating field strains.

Allium-Based Phytobiotic Enhances Egg Production in Laying Hens through Microbial Composition Changes in Ileum and Cecum.

Phytobiotics (bioactive compounds extracted from plants) are one of the explored alternatives to antibiotics in poultry and livestock due to their antimicrobial activity and its positive effects on gut microbiota and productive properties. In this study, we supplemented a product based on garlic and onion compounds in the diet to laying hens at the beginning of their productive life (from 16 to 20 weeks post-hatching). The experimental group showed a significant increase in the number of eggs laid and in their size, produced in one month compared to the control. This increase in production was accompanied by microbiota changes in the ileum and cecum by means of high throughput sequencing analyses. These bacterial shifts in the ileum were mainly the result of compositional changes in the rare biosphere (unweighted UniFrac), while in the cecum, treatment affected both majority and minority bacterial groups (weighted and unweighted UniFrac). These changes in the microbiota suggest an improvement in food digestibility. The relative abundance of Lactococcus in the ileum and Lactobacillus in the cecum increased significantly in the experimental group. The relative abundance of these bacterial genera are known to have positive effects on the hosts. These results are very promising for the use of these compounds in poultry for short periods.

The Use of Computer Records: A Tool to Increase Productivity in Dairy Herds.

The level of adoption of herd management information system veterinary automated management and production control program (VAMPP) Bovine and its impact on productivity of 912 specialized dairy systems with at least 5 years of information recorded was studied. Herds were classified as low (n = 389), medium (n = 343), or high (n = 180) adoption level on the basis of extent and consistency of record keeping for variables related to production, reproduction, and health. For each herd, within-year averages were obtained for six performance traits: age at first calving (AFC), days open (DO), daily milk yield (DMY), productive life (PL), incidence of clinical mastitis (MAST), and incidence of lameness (LAM). These variables were investigated with a generalized linear mixed model that included the fixed effects of the adoption level, follow-up year, and their interaction, adjusted by the fixed effects of herd size, agroecological zone, calendar period, breed group, and the random effects of variation between/within herds. A significant effect of the adoption level over all the variables was observed, except DMY and PL. The follow-up year was significant for all the variables except LAM. There were marked reductions for AFC and DO in the first 4 years of follow-up. AFC was higher and DO shorter in the low compared to medium and high level of adoption herds (p < 0.001). DMY showed a significant increasing trend (p < 0.001), regardless of the adoption level. There was higher MAST and LAM incidence in the higher adoption level (p < 0.05). The economic benefit was estimated through a stochastic simulation model using an approach based in partial budget analysis. For a herd with a medium level of adoption, the change in gross margin (GMMIS $USD) and marginal return rate (MRRMIS %) for the first 5 years of use of the system was estimated. Under these conditions, there was a GMMIS of $6890 and MRRMIS of 163%. Variation of ±10% in DMY and DO caused changes in the GMMIS of ±$1000 and ±$110, respectively, and in MRRMIS ±24% and ±4%, respectively. The trends suggest a positive influence of VAMPP in productive and reproductive traits during the first years of implementation, with less benefit for the low adoption levels.

Antibacterial and Antifungal Activity of Propyl-Propane-Thiosulfinate and Propyl-Propane-Thiosulfonate, Two Organosulfur Compounds from Allium cepa: In Vitro Antimicrobial Effect via the Gas Phase.

Propyl-propane thiosulfinate (PTS) and propyl-propane thiosulfonate (PTSO) are two volatile compounds derived from Allium cepa with a widely documented antimicrobial activity. The aim of this study was to evaluate their anti-candidiasis activity and the ability of its gaseous phase to inhibit bacterial and yeast growth in vitro. The minimum inhibitory concentration of various antifungal products (including PTS and PTSO) was determined versus 203 clinical isolates of Candida spp. through broth microdilution assay. Additionally, the antimicrobial activity through aerial diffusion of PTS and PTSO was evaluated over the growth of a collection of bacteria and yeasts cultivated in agar plates. All yeasts were susceptible to the antifungals tested, except C. glabrata and C. krusei, that showed azole resistance. PTSO (MIC50 and MIC90 ranged from 4 to 16 mg/L and 8 to 32 mg/L, respectively) was significantly more active against yeasts than PTS (MIC50 and MIC90 ranged from 16 to 64 mg/L and 32 to 64 mg/L). Values were higher than those obtained for antifungal drugs. Gaseous phases of PTS and PTSO generated growth inhibition zones whose diameters were directly related to the substances concentration and inversely related to the microbial inoculum. The quantification of PTS and PTSO levels reached in the growth media through aerial diffusion displayed a concentration gradient from the central zone to the periphery. Only P. aeruginosa ATCC 27853 showed resistance, while yeasts (C. albicans ATCC 200955 and C. krusei ATCC 6258) presented the higher susceptibility to both compounds. These results suggest that PTS and PTSO display antibacterial and anti-candidiasis activity in vitro through aerial diffusion, having potential use in human therapy.

Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells.

Microtubule-dependent motors usually work together to transport organelles through the crowded intracellular milieu. Thus, transport performance depends on how motors organize on the cargo. Unfortunately, the lack of methodologies capable of measuring this organization in cells determines that many aspects of the collective action of motors remain elusive. Here, we combined fluorescence fluctuations and single particle tracking techniques to address how kinesins organize on rod-like mitochondria moving along microtubules in cells. This methodology simultaneously provides mitochondria trajectories and EGFP-tagged kinesin-1 intensity at different mitochondrial positions with millisecond resolution. We show that kinesin exchange at the mitochondrion surface is within ~100 ms and depends on the organelle speed. During anterograde transport, the mitochondrial leading tip presents slower motor exchange in comparison to the rear tip. In contrast, retrograde mitochondria show similar exchange rates of kinesins at both tips. Numerical simulations provide theoretical support to these results and evidence that motors do not share the load equally during intracellular transport.

Egg Production in Poultry Farming Is Improved by Probiotic Bacteria.

Antimicrobial resistance (AMR) is one of the most serious threats for human health in the near future. Livestock has played an important role in the appearance of antibiotic-resistant bacteria, intestinal dysbiosis in farming animals, or the spread of AMR among pathogenic bacteria of human concern. The development of alternatives like probiotics is focused on maintaining or improving production levels while diminishing these negative effects of antibiotics. To this end, we supplied the potential probiotic Enterococcus faecalis UGRA10 in the diet of laying hens at a final concentration of 108 Colony Forming Units per gram (CFU/g) of fodder. Its effects have been analyzed by: (i) investigating the response of the ileum and caecum microbiome; and (ii) analyzing the outcome on eggs production. During the second half of the experimental period (40 to 76 days), hens fed E. faecalis UGRA10 maintained egg production, while control animals dropped egg production. Supplementation diet with E. faecalis UGRA10 significantly increased ileum and caecum bacterial diversity (higher bacterial operational taxonomic unit richness and Faith's diversity index) of laying hens, with animals fed the same diet showing a higher similarity in microbial composition. These results point out to the beneficial effects of E. faecalis UGRA10 in egg production. Future experiments are necessary to unveil the underlying mechanisms that mediate the positive response of animals to this treatment.

In Vitro Antibacterial Activity of Propyl-Propane-Thiosulfinate and Propyl-Propane-Thiosulfonate Derived from Allium spp. against Gram-Negative and Gram-Positive Multidrug-Resistant Bacteria Isolated from Human Samples.

BACKGROUND: The aim of this study was to compare the in vitro antibacterial activity of two compounds derived from Alliaceae, PTS (propyl-propane-thiosulfinate), and PTSO (propyl-propane-thiosulfonate), with that of other antibiotics commonly used against bacteria isolated from humans. MATERIALS AND METHODS: A total of 212 gram-negative bacilli and 267 gram-positive cocci isolated from human clinical samples and resistant to at least one group of antibiotics were selected. In order to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) to various antibiotics as well as PTS and PTSO, all isolates underwent broth microdilution assay. RESULTS: PTS showed moderate activity against Enterobacteriaceae with MIC50 (and MBC50) and MIC90 (and MBC90) values of 256-512 mg/L, while PTSO showed greater activity with MIC50 and MIC90 values of 64-128 mg/L and MBC50 and MBC90 values of 128-512 mg/L. These data show the bactericidal activity of both compounds and indicate that PTSO was more active than PTS against this group of bacteria. Both compounds showed lower activity against P. aeruginosa (MIC50 = 1024 mg/L, MIC90 = 2048 mg/L, MBC50 = 2048 mg/L, and MBC90 = 2048 mg/L, for PTS; MIC50 = 512 mg/L, MIC90 = 1024 mg/L, MBC50 = 512 mg/L, and MBC90 = 2048 mg/L, for PTSO) compared to those obtained in others nonfermenting gram-negative bacilli (MIC50 = 128 mg/L, MIC90 = 512 mg/L, MBC50 = 128 mg/L, and MBC90 = 512 mg/L, for PTS; MIC50 = 64 mg/L, MIC90 = 256 mg/L, MBC50 = 64 mg/L, and MBC90 = 256 mg/L, for PTSO) and also indicate the bactericidal activity of both compounds against these groups of bacteria. Finally, the activity against S. aureus, E. faecalis, and S. agalactiae was higher than that observed against enterobacteria, especially in the case of PTSO (MIC50 = 8 mg/L, MIC90 = 8 mg/L, MBC50 = 32 mg/L, and MBC90 = 64 mg/L, in S. aureus; MIC50 = 4 mg/L, MIC90 = 8 mg/L, MBC50 = 8 mg/L, and MBC90 = 16 mg/L, in E. faecalis and S. agalactiae). CONCLUSION: PTS and PTSO have a significant broad spectrum antibacterial activity against multiresistant bacteria isolated from human clinical samples. Preliminary results in present work provide basic and useful information for development and potential use of these compounds in the treatment of human infections.

Imaging transcription factors dynamics with advanced fluorescence microscopy methods.

Pluripotent stem cells (PSCs) are capable of self-renewing and producing all cell types derived from the three germ layers in response to developmental cues, constituting an important promise for regenerative medicine. Pluripotency depends on specific transcription factors (TFs) that induce genes required to preserve the undifferentiated state and repress other genes related to differentiation. The transcription machinery and regulatory components such as TFs are recruited dynamically on their target genes making it essential exploring their dynamics in living cells to understand the transcriptional output. Non-invasive and very sensitive fluorescence microscopy methods are making it possible visualizing the dynamics of TFs in living specimens, complementing the information extracted from studies in fixed specimens and bulk assays. In this work, we briefly describe the basis of these microscopy methods and review how they contributed to our knowledge of the function of TFs relevant to embryo development and cell differentiation in a variety of systems ranging from single cells to whole organisms.

Selection of talents in handball: anthropometric and performance analysis / Seleção de talentos em handebol: análise antropométrica e de desempenho / Selección de talentos en el balonmano: análisis antropométrico y de rendimiento

ABSTRACT Introduction: Anthropometric and physical performance parameters that determine competitive levels in handball need to be carefully studied to identify which of them can bring the optimal contribution when the talents for this sport are selected. Objective: To identify which anthropometric and physical performance variables evaluated in the basic categories (infantile, under-15 and cadet, under-17) have the greatest influence on professional levels attained by male and female handball players throughout their sports careers. Method: A total of 145 handball players (75 males and 70 females) participated in the study. Participants were initially evaluated during the season 1998-99 and their performance levels were supervised until March 2013. The resulting data were classified according to the performance level (regional n = 109; national n = 36), gender, and category (infantile, cadet). Results: The MANCOVA analysis indicated that the player's maturation stage is one of the main parameters to be accounted for when selecting the variables that should configure a talent detection model for handball, which is also conditioned by sex. Anthropometric variables are highly influential in the case of male players, while physical performance variables (squat jump, counter movement jump, counter movement jump with arm, 10x5m shuttle run, 20 m shuttle run, and VO2 max) have a similar effect in males and females. Conclusion: The study of changes occurring in anthropometric and physical performance variables may yield useful information to detect talents in handball, and maturation is a key factor to choose the most appropriate variables.

RESUMO Introdução: Os parâmetros antropométricos e de desempenho físico que determinam o nível competitivo no handebol devem ser submetidos a um estudo mais aprofundado para identificar quais deles podem contribuir de forma otimizada ao serem selecionados os talentos para esse esporte. Objetivo: Identificar quais variáveis antropométricas e de desempenho físico avaliadas nas categorias de base (infantil, sub-15 e cadete, sub-17) têm a maior influência sobre os níveis profissionais atingidos por jogadores de handebol do sexo masculino e feminino ao longo de sua carreira esportiva. Método: Um total de 145 jogadores de handebol (75 homens e 70 mulheres) participou do estudo. Os participantes foram inicialmente avaliados durante a temporada de 1998-99 e os níveis de desempenho foram supervisionados até março de 2013. Os dados resultantes foram classificados pelo nível de desempenho (regional n = 109; nacional n = 36), por sexo e por categoria (infantil, cadete). Resultados: A análise MANCOVA indicou que o estágio de maturação do jogador é um dos principais parâmetros a serem considerados ao escolher quais variáveis devem configurar o modelo de detecção de talento para o handebol, que também é condicionado por sexo. As variáveis antropométricas influenciam muito os atletas do sexo masculino, enquanto as variáveis de desempenho físico (salto agachado (squat jump), salto com contramovimento (counter movement jump), salto com contramovimento com braço (counter movement jump with arm), corrida vaivém (shuttle run) 10 x 5 m, corrida vaivém 20 m e VO2 máx) têm efeito semelhante em ambos os sexos. Conclusão: O estudo das mudanças que ocorrem em variáveis de desempenho antropométricas e físicas podem gerar informações úteis para identificar talentos para o handebol, e a maturação é um fator chave na escolha das variáveis mais adequadas.

RESUMEN Introducción: Los parámetros antropométricos y de rendimiento físico que determinan el nivel competitivo en el balonmano deben ser sometidos a un estudio más profundo con el fin de identificar cuáles de ellos pueden contribuir de forma óptima para la selección de talentos para ese deporte. Objetivo: Identificar qué variables antropométricas y de rendimiento físico evaluadas en las categorías base (infantil, sub-15 y cadete, sub-17) tienen mayor influencia sobre los niveles profesionales alcanzados por los jugadores y las jugadoras de balonmano a lo largo de su carrera deportiva. Método: Un total de 145 jugadores de balonmano (75 hombres y 70 mujeres) participaron en el estudio. Los participantes fueron evaluados inicialmente durante la temporada 1998-99 y los niveles de rendimiento fueron supervisados hasta marzo de 2013. Los datos fueron clasificados según el nivel de rendimiento (regional n = 109; nacional n = 36), el sexo y la categoría (infantil, cadete). Resultados: El análisis MANCOVA indicó que la etapa de maduración del jugador es uno de los principales parámetros a tener en cuenta al seleccionar qué variables deben componer el modelo identificador del talento en balonmano, que también está condicionada por el sexo. Las variables antropométricas son las que mayor influencia presentan en los deportistas de sexo masculino, mientras que las variables de rendimiento físico (sentadilla con salto (squat jump), salto en contramovimiento (counter movement jump), salto en contramovimiento con brazos (counter movement jump with arm), 10 x 5 m shuttle run, 20 m shuttle run y VO2 máx) tienen una influencia similar en ambos sexos. Conclusión: El estudio de los cambios que se producen en las variables antropométricas y de rendimiento físico puede generar informaciones útiles para la identificación de talentos en el balonmano, y la maduración es un factor clave a la hora de escoger las variables más adecuadas.

Updates in Mirizzi syndrome.

Mirizzi syndrome, known as extrinsic bile compression syndrome, is a rare complication of cholecystitis and chronic cholelithiasis, secondary to the obliteration of the infundibulum of the gallbladder or cystic duct caused by the impact of one or more calculations in these anatomical structures, which leads to compression of the adjacent bile duct, resulting in partial or complete obstruction of the common hepatic duct, triggering liver dysfunction. Our aim is to identify and describe the current epidemiology, diagnostic methods, and treatment of Mirizzi syndrome. A literature search was performed using different databases, including Medline, Cochrane, Embase, Medscape, PubMed, using keywords: Mirizzi syndrome, epidemiology, markers, pathophysiology, clinical presentation, diagnosis, and treatment. Selected original articles, review articles or case reports from 1997 to 2015 were collected, written in English or Spanish. The endoscopic retrograde cholangiopancreatography (ERCP) is the most accurate diagnostic method. The traditional treatment has been surgery and involves an incision at the bottom of the gallbladder and calculus removal. If fistulas are observed, it is performed a partial cholecystectomy; otherwise, a cholecystocholedochoduodenostomy is an alternative. Endoscopic treatment includes biliary drainage and stone extraction. Many surgeons claim that laparoscopic cholecystectomy is contraindicated in Mirizzi syndrome because of the presence of inflammatory tissue and adhesions in the Calot's triangle. If dissection is attempt, it can cause unnecessary injury to the bile duct. However, other surgeons consider the laparoscopic approach is feasible, although technically challenging. Currently, laparoscopic cholecystectomy for this condition is considered controversial and technically challenging; however, it has shown that with the right skills and equipment, it is a safe and feasible way to treat some cases of Mirizzi syndrome type I and II.

Análisis de las conductas de los entrenadores después del tiro en baloncesto / Análise do comportamento dos treinadores no basquete após o disparo / Analysis of the behavior of coaches in basketball after shot

La presente investigación analiza las conductas de los entrenadores de baloncesto después del lanzamiento a canasta (tanto de los jugadores de su equipo como del equipo contrario) en situaciones de competición en categorías de formación. Intervinieron en el presente estudio tres entrenadores, todos poseen la titulación española oficial necesaria para entrenar en las diferentes categorías. Se han analizado 9 partidos oficiales de la Liga Gallega (3 por cada uno de los 3 entrenadores participantes) y se registraron 1196 conductas tras lanzamiento. El instrumento utilizado para la recogida de datos procede de una adaptación del Coach Behavioral Assessment System (Smith, Smoll y Hunt, 1977; Cruz, 1989) y del Sistema de Observación de las Conductas del Entrenador (Montero, 2004). Tras los resultados obtenidos, se puede afirmar que las conductas de inhibición ('ignorar el error' y 'no reforzar') son las más frecuentes y que el resultado en el marcador está asociado a cambios en el comportamiento de los entrenadores (AU)

O presente trabalho analisa o comportamento dos treinadores de basquete após arremesso à cesta (dos jogadores de sua equipe e da equipe adversária) em situações competitivas nas categorias de formação do basquetebol. São participantes deste estudo três treinadores, todos com as certificações necessárias para ser treinador em diferentes categorias na Espanha. Foram analisados nove jogos oficiais da Liga Gallega (três de cada um dos três treinadores participantes) e 1.196 observações foram feitas após arremessos. O instrumento utilizado para a coleta de dados foi adaptado do Coach Behavioral Assessment System (Smith, Smoll e Hunt, 1977; Cruz, 1989) e Sistema de Observación de las Conductas del Entrenador (Montero, 2004). Como resultados, observou-se que a conduta de inibição ('ignorar erro' e 'não reforçar') são os mais frequentes, e resultado no placar influencia o comportamento dos treinadores (AU)

The study presented below is an analysis of the behavior of basketball coaches after the shot (players of both teams) during the competition in youth categories. In this study, three coaches participed, everybody have the official Spanish qualifications needed to train in different categories. It was analyzed 9 official games of the Galician league (3 each of the 3 coaches participants) and 1196 behaviors were registered after shot. The instrument used for the data collection was an adaptation of the Coach Behavioral Assessment System (Smith, Smoll & Hunt, 1977) and of the Sistema de Observación de las Conductas del Entrenador (Montero, 2004). After the results obtained, we can say that the inhibition behaviors (ignore mistake and not reinforcement) are the most frequently and that the score is associated with changes in the behaviors of coaches (AU)

Choline sulfatase from Ensifer (Sinorhizobium) meliloti: Characterization of the unmodified enzyme.

Ensifer (Sinorhizobium) meliloti is a nitrogen-fixing α-proteobacterium able to biosynthesize the osmoprotectant glycine betaine from choline sulfate through a metabolic pathway that starts with the enzyme choline-O-sulfatase. This protein seems to be widely distributed in microorganisms and thought to play an important role in their sulfur metabolism. However, only crude extracts with choline sulfatase activity have been studied. In this work, Ensifer (Sinorhizobium) meliloti choline-O-sulfatase was obtained in a high degree of purity after expression in Escherichia coli. Gel filtration and dynamic light scattering experiments showed that the recombinant enzyme exists as a dimer in solution. Using calorimetry, its catalytic activity against its natural substrate, choline-O-sulfate, gave a k cat=2.7×10-1 s-1 and a K M=11.1 mM. For the synthetic substrates p-nitrophenyl sulfate and methylumbelliferyl sulfate, the k cat values were 3.5×10-2 s-1 and 4.3×10-2 s-1, with K M values of 75.8 and 11.8 mM respectively. The low catalytic activity of the recombinant sulfatase was due to the absence of the formylglycine post-translational modification in its active-site cysteine 54. Nevertheless, unmodified Ensifer (Sinorhizobium) meliloti choline-O-sulfatase is a multiple-turnover enzyme with remarkable catalytic efficiency.

Oxigenoterapia domiciliaria en las enfermedades pulmonares obstructivas autores / No disponible

No disponible

Microwave-assisted synthesis, microstructure, and physical properties of rare-earth chromites.

The full rare-earth (RE) chromites series (RE)CrO(3) with an orthorhombic distorted (Pnma) perovskite structure and the isostructural compound YCrO(3) can be synthesized through a simple microwave-assisted technique, yielding high-quality materials. Magnetization measurements evidence that the Néel temperature for antiferromagnetic Cr(3+)-Cr(3+) ordering strongly depends on the RE(3+) ionic radius (IOR), and a rich variety of different magnetic spin interactions exists. Dielectric spectroscopy on sintered pellets indicates electronic inhomogeneity in all samples as manifested by the presence of at least two dielectric relaxation processes associated with grain boundary and grain interior bulk contributions. X-ray diffraction, Raman spectroscopy, and temperature-dependent dielectric permittivity data do not indicate potential noncentrosymmetry in the crystal or concomitant ferroelectricity. Strong correlations between the magnetic and dielectric properties were not encountered, and microwave-synthesized (RE)CrO(3) may not be classified as magnetoelectric or multiferroic materials.

Measuring home advantage in Spanish handball.

Since Pollard established the system for analysing home advantage in 1986, it has been demonstrated and quantified in various sports, including many team sports. This study aims to assess whether home advantage exists in handball, using a sample of more than 19,000 Spanish handball league games. Results of the games played at home and away, the sex of the players, and the levels of the competition were included as variables. In Spanish handball, there was a home advantage of 61%, which means, on average, the team playing at home wins 61% of points available. This value varies according to sex and according to competition level, increasing as competition level decreases and season rank improves.

Sindrome de Waardenburg: a propósito de un caso / Waardenburg syndrome: on the subject of a case

El sindrome de Waardenburg es un desorden pigmentario asociado a hipoacusia neurosensorial bilateral. Reportamos el caso de una niña de 4 años de edad que ingresa al servicio de pediatria por un cuadro urinario al examen fisico llama la atención el azuel brillante del iris en ambos ojos, también presenta hipoacusia bilateral. Analizando los rasgos de la paciente se concluye en un sindrome de Waardenburg, poco común en nuestro medio: por lo que se realiza una revisión bibliográfica acerca del caso.

Correlation between the structure and the piezoelectric properties of lead-free (K,Na,Li)(Nb,Ta,Sb)O3 ceramics studied by XRD and Raman spectroscopy.

This article reviews on the use of Raman spectroscopy for the study of (K,Na,Li)(Nb,Ta,Sb)O(3) lead-free piezoceramics. Currently, this material appears to be one of the most interesting and promising alternatives to the well-known PZT piezoelectric materials. In this work, we prepare piezoceramics with different stoichiometries and study their structural, ferroelectric, and piezoelectric properties. By using both Raman spectroscopy and X-ray diffraction, we establish a direct correlation between the structure and the properties. The results demonstrate that the wavenumber of the A(1g) vibration is proportional to the tetragonality, the remnant polarization, and the piezoelectric coefficients of these materials. Thus, Raman spectroscopy appears as a very useful technique for a fast evaluation of the crystalline structure and the ferroelectric/ piezoelectric properties.

Serological detection of viral infections in captive wild cats from costa rica.

Serum samples from a total of 44 wildcats, 28 margays (Leopardus wiedii), 10 ocelots (Leopardus pardalis), four jaguaroundis (Herpailurus yaguaroundi), one oncilla (Leopardus tigrina), and one jaguar (Panthera onca) were obtained between January 2001 and August 2002 from the Profelis Centre for rehabilitation of wild felids, located in the northwestern region of Costa Rica. Forty three samples were tested for antibodies against feline immunodeficiency virus (FIV) and p27 antigen of feline leukemia virus (FeLV), 42 samples for antibodies against feline parvovirus (FPV), and 30 for antibodies against feline calicivirus (FCV). None of the samples contained detectable antibodies against FIV or p27 antigen of FeLV, all samples contained antibodies against FPV, and one sample contained antibodies against FCV.

Porcentaje en ictericia neonatal en el Hospital Arco Iris durante la gestión 2008 a 2009 / Percentage of neonatal jaundice at the Arco Iris Hospital from 2008 to 2009

La ictericia en el recién nacido (RN) , es un hecho fisiológico de forma general, causada por una hiperbilirrubinea de predminio indirecto secundario a madurez hepática e hiperproducción de bilirrubinea. La ictericia no fisiologica, se producen en las primeras 24 horas de vida, en presencia de un incremento superior a los 0,5 mg por ciento por hora o los 5 mg porciento diarios, o bien, en caso de que supere los 15 mg por ciento o 10 mg por ciento en neonatos a término y pretérmiino respectivamente. El presente estudio se realizó en el Serivicio de Neonatologia del Hospital Arco iris, mediante la recolección de datos del cuaderno de registro de ingresos del servicio de los años 2008 y 2009. De los 330 neonatos tomados para el estudio 180 pertenecen al año 2008 y 170 al año 2009, evidenciandose un incremento de casos en un periodo de un año. En forma independietne representan un 27 por ciento y un 28 por ciento respectivamente. De ello, el flujo de pacientes que ingresan al Servicio de Neonatologoia con esta patología es de alrededor de 27.5 por ciento de los ingresados al Servicio indicando una gran prevalencia en nuestro medio el cual carece de educación en cuanto a cuidados del recién nacido.