ABSTRACT
Allelic frequencies and haplotypic composition of 305 male unrelated individuals from the Caribbean Colombian states of Atlántico, Bolívar, Cesar, Córdoba, Guajira, Magdalena and Sucre, were determined using 16 Y-chromosome STR loci. Two hundred and ninety three (293) haplotypes were identified, of which 283 were unique and the other 10 were found twice or thrice in the Caribbean population tested. Haplotypic diversity surpassed the values obtained in other populations, ranging from 99.66% in the population of Sucre to 99.99% in the population of Córdoba. We also calculated the overall haplotypic diversity (99.97%) and the discrimination power of these haplotypes (96.1%) in these groups. Analysis of molecular variance (AMOVA) for 10 Colombian and Spanish populations (3139 haplotypes) reveals low differentiation between the Colombian populations of mainly European descent and large distance to Afroamerican populations living in Colombia.
Subject(s)
Chromosomes, Human, Y , Genetics, Population , Microsatellite Repeats/genetics , Alleles , Black People/genetics , Colombia , DNA/genetics , DNA/isolation & purification , DNA Fingerprinting/methods , Gene Frequency , Geography , Haplotypes , Humans , Male , Polymerase Chain Reaction , Quality Control , White People/geneticsABSTRACT
Genotype polymorphism studies at the 13 loci STRs included in the combined DNA index system [CODIS and PCR-based short tandem repeat loci, in: Proceedings of the Second European Symposium on Human Identification, Promega Corporation, Madison, WI, 1998, pp. 73-88; J. Forensic Sci. 46 (2001) 453] (CODIS: D3S1358, HUMvWA31, HUMFGA, D8S1179 D21S11, D18S51, D5S818, D13S317, D7S820, HUMTH01, HUMTPOX, HUMCSF1PO and D16S539) were carried out in a sample of 1429 unrelated Colombian individuals belonging to 25 different departments. As many other countries in Latino-America, Colombia shows an important admixture component, basically integrated by Amerindians, European-descendants and African-descendants. Due to the fact that only partial population analyses have been carried out in the country, the main aim of the present analysis is to establish a database of forensic interest based on the widely used CODIS systems covering the main Colombian regions.
Subject(s)
Gene Frequency , Genetics, Population , Polymorphism, Genetic , Tandem Repeat Sequences , Colombia , DNA Fingerprinting/methods , Ethnicity/genetics , Genotype , Humans , Polymerase Chain ReactionABSTRACT
In this study, the use of Mtp-40 and alpha antigen polymerase chain reaction (PCR) amplification fragments for the precise tuberculosis (TB) diagnosis was evaluated. One hundred and ninety two different samples were obtained from 113 patients with suspected TB. Mtp-40 and alpha antigen protein genes were amplified by the PCR technique and compared to both the "gold standard" (culture) test, as well as the clinical parameters (including a clinical record and X-ray film exam in 113 patients). Thirty-eight of the 113 patients had a presumptive clinical diagnosis of TB; 74 percent being detected by PCR technique, 58 percent by culture and 44 percent by direct microscopic visualization. Weconclude that it is possible to use PCR as a suitable technique for the detection of any mycobacteria by means of the alpha antigen product, or the specific infection of Mycobacterium tuberculosis by means of the mtp-40 gene. This might be a good supporting tool in difficult clinical TB diagnosis and pauci-bacillary cases
Subject(s)
Humans , Antigens, Bacterial , Bacterial Proteins , Mycobacterium tuberculosis , Tuberculosis , Antigens, Bacterial , Bacterial Proteins , Colombia , Evaluation Study , Gene Amplification , Mycobacterium tuberculosis , Polymerase Chain Reaction , Sensitivity and SpecificityABSTRACT
In this study, the use of Mtp-40 and alpha antigen polymerase chain reaction (PCR) amplification fragments for the precise tuberculosis (TB) diagnosis was evaluated. One hundred and ninety two different samples were obtained from 113 patients with suspected TB. Mtp-40 and alpha antigen protein genes were amplified by the PCR technique and compared to both the "gold standard" (culture) test, as well as the clinical parameters (including a clinical record and X-ray film exam in 113 patients). Thirty-eight of the 113 patients had a presumptive clinical diagnosis of TB; 74% being detected by PCR technique, 58% by culture and 44% by direct microscopic visualization. Weconclude that it is possible to use PCR as a suitable technique for the detection of any mycobacteria by means of the alpha antigen product, or the specific infection of Mycobacterium tuberculosis by means of the mtp-40 gene. This might be a good supporting tool in difficult clinical TB diagnosis and pauci-bacillary cases.
