ABSTRACT
The structure of the O-antigen chain of the lipopolysaccharide isolated from Rhizobium tropici CIAT899, by the phenol-water procedure, and recovered from the phenol layer, has been investigated by hydrolysis, methylation analysis and 1D and 2D 1H and 13C NMR spectroscopy of the complete polysaccharide and of oligosaccharides obtained by partial hydrolysis. The O-antigen has the repeating unit [formula: see text]
Subject(s)
O Antigens/chemistry , Rhizobium/immunology , Carbohydrate Sequence , Hydrolysis , Magnetic Resonance Spectroscopy , Methylation , Molecular Sequence Data , Monosaccharides/analysis , Oligosaccharides/chemistryABSTRACT
The lipid moieties of two lipid A's isolated from the phenolic and aqueous fractions of lipopolysaccharide from Rhizobium tropici CIAT899 have been studied. Several 3-hydroxy fatty acids and two long-chain hydroxy fatty acids, 27-hydroxyoctacosanoic acid, and 29-hydroxytriacontanoic acid were identified; the ratios of these acids are the same in both lipid A's. These results can be used for chemotaxonomic purposes.
