ABSTRACT
It is shown that the dynamics of lipid peroxidation (LPO) in patients with purulent meningitis is unspecified in character and is encountered both in meningococcal and pneumococcal infection. The level of secondary LPO products and the degree of hyperfermentemia, which are determined according to the dynamics of changes in the activity of aspartate transaminase, are objective characteristics of the severity of the patient's condition and reach maximum on the 5th day of the disease. The correlation between the dynamics of the primary LPO products and the ceruloplasmin/transferin coefficient allows the severity of the disease to be prognosticated.
Subject(s)
Lipid Peroxidation , Lipid Peroxides/blood , Meningitis, Meningococcal/blood , Meningitis, Pneumococcal/blood , Adult , Aged , Humans , Malondialdehyde/blood , Middle Aged , Prognosis , Time FactorsABSTRACT
Ceruloplasmin and transferrin blood serum levels were measured during bacterial infection of the CNS. A decrease in ceruloplasmin level and a gradual increase in transferrin level were observed on the 5th day of the disease. The minimum ceruloplasmin level was observed simultaneously with the maximum MDA and GOT levels. There was a close correlation between the time course of ceruloplasmin, transferrin, MDA and GOT changes and the clinical condition of the patient.
Subject(s)
Central Nervous System Diseases/blood , Ceruloplasmin/analysis , Lipid Peroxides/blood , Meningococcal Infections/blood , Pneumococcal Infections/blood , Transferrin/blood , Adult , Aspartate Aminotransferases/blood , Central Nervous System Diseases/diagnosis , Clinical Enzyme Tests , Humans , Malondialdehyde/blood , Meningococcal Infections/diagnosis , Middle Aged , Pneumococcal Infections/diagnosis , Prognosis , Time FactorsABSTRACT
It was found that the increase in the serum level of lipid peroxidation products in patients with meningococcal meningitis correlated with the severity of the disease. The dependence between the level of lipid peroxidation products and the activity of cell enzymes in the serum has been investigated. During the first day of the disease GOT activity was strongly increased, while the level of lipid peroxidation products was 1560 +/- 237 mumol/l, which may be of a diagnostic value.
Subject(s)
Aspartate Aminotransferases/blood , Creatine Kinase/blood , Lipid Peroxides/blood , Meningitis, Meningococcal/blood , Adult , Brain Edema/blood , Brain Edema/etiology , Cytoplasm/enzymology , Humans , Malondialdehyde/blood , Meningitis, Meningococcal/complications , Middle Aged , Oxidation-Reduction , Shock, Septic/blood , Shock, Septic/etiology , Time FactorsABSTRACT
To assess the efficacy of laser therapy in the management of unresponding wounds and ulcers the method based on the estimation of chemiluminescence of tissue fluid in the presence of H2O2 was developed. It is suggested that laser radiation-induced decrease in chemiluminescence can be attributed to the activation of catalase in tissue fluid, which has an obvious positive therapeutic effect on reparative regeneration of wounds and ulcers.
Subject(s)
Exudates and Transudates/radiation effects , Hydrogen Peroxide/radiation effects , Laser Therapy , Lipid Peroxides/radiation effects , Wounds and Injuries/radiotherapy , Animals , Catalase/metabolism , Catalase/radiation effects , Exudates and Transudates/metabolism , Hydrogen Peroxide/metabolism , Lipid Peroxides/metabolism , Luminescent Measurements , Wound Healing/radiation effects , Wounds and Injuries/metabolismABSTRACT
The authors have used the method of induced chemoluminescence of the wound release for an objective assessment of the course of the wound process and the efficiency of the chosen treatment. The investigations were performed in 20 children with postoperative and posttraumatic wounds of soft tissues.
Subject(s)
Hydrogen Peroxide , Wound Healing , Wounds, Penetrating/therapy , Adolescent , Child , Child, Preschool , Humans , Infant , Infant, Newborn , Laser Therapy , Luminescent Measurements , Surgical Wound Infection/therapy , Wounds, Penetrating/physiopathology , Wounds, Penetrating/surgeryABSTRACT
Activity of potassium ions was examined in 3 liquid media of 54 rabbit cadavers by potentiometry with the use of a potassium-selective valinomycin electrode. The reported and obtained data were used for the development of the diagnostic regression equations and the curves to define the time of death from the activity of a complex of 3 ions (potassium, sodium and hydrogen) in the blood serum, CSF and vitreous body fluid. The method is offered for appropriate trials in human cadavers.
Subject(s)
Death , Forensic Medicine , Potassium/analysis , Animals , Ions , Potassium/blood , Potassium/cerebrospinal fluid , Potentiometry , Rabbits , Vitreous Body/analysisABSTRACT
The paper is concerned with a procedure for determination of the death onset. The time course of changes in the activity of sodium and hydrogen ions in the blood, CSF and vitreous body was examined in 42 rabbit cadavers. The animals were killed by air embolism. Before material collection the rabbit cadavers were stored at a temperature of 17-20 degrees C and a relative humidity of 40-60%. The material was collected promptly after the killing (0 h--control) and then 2, 4, 6, 12, 18, 24, 36 and 48 hours after the death onset. The activity of sodium ions was determined potentiometrically with the use of an ion-selective sodium glass electrode, whereas that of hydrogen ions with the aid of a set up manufactured by the Radiometer Company. The results indicate the advisability of using the procedure described for studies on section material to devise criteria of expert evaluation of the time of the death onset.
