ABSTRACT
In order to obtain information about blood and cerebrospinal fluid (CSF) concentrations, and CSF/blood ratio data of fuel related substrates at the end of a prolonged fast in children, we have selected biochemical data from fasting test procedures in 11 control children aged 3-5 yr, fasted 24 h, and 58 control children aged 6-15 yr, fasted 40 h. There was a good correlation between blood and CSF concentrations for glucose, acetoacetate and beta-hydroxybutyrate. The relation with age and sex has been analyzed only in the older children. CSF and blood values for glucose are positively related with age, and both ketones are negatively related with age. Lactate, pyruvate and alanine concentrations in blood and CSF are not related with age, except for CSF pyruvate. With respect to the CSF/blood ratio for the above mentioned components, only the value for acetoacetate is sex and age related. The calculated median caloric values for the sum of glucose, lactate, pyruvate and ketones in CSF are independent of age at the end of a 40-h fast. The diminished glucose contribution on the CSF caloric homeostasis in younger children is fully compensated by the ketone bodies.
Subject(s)
Acetoacetates/metabolism , Blood Glucose/metabolism , Energy Metabolism , Fasting , Hydroxybutyrates/metabolism , Acetoacetates/blood , Acetoacetates/cerebrospinal fluid , Adolescent , Aging/metabolism , Blood Glucose/cerebrospinal fluid , Child , Child, Preschool , Female , Homeostasis , Humans , Hydroxybutyrates/blood , Hydroxybutyrates/cerebrospinal fluid , Male , Sex FactorsABSTRACT
In order to study the relationship between sex, age and glucose, and the concentrations of various fuel related blood substrates in children during prolonged fasting, we have selected data of fasting procedures in 13 control children aged 3-5 yr, fasted 24 h, and 58 control children aged 6-15 yr, fasted 40 h. Compared to the blood results after overnight fast, glucose is decreased, and lactate, pyruvate, ketones and non-esterified fatty acids (NEFA's) are all clearly increased at the end of fast. The concentrations of alanine and triglycerides remain unchanged. The relation with sex, age and glucose has only been analyzed in the older children group. A sex-dependency is indicated for the ketones. Ketones are negatively related with age. NEFA's pyruvate and alanine are not age-related, whereas glucose, lactate and triglycerides are moderately age-dependent. Ketones are negatively related with glucose, whereas pyruvate, NEFA's and triglycerides are not glucose-related. Lactate and alanine are weakly related to glucose. The data demonstrate diminished glucose homeostasis and increased ketogenesis in younger children compared to older ones during prolonged fasting.
Subject(s)
Energy Metabolism , Fasting , Adolescent , Age Factors , Alanine/blood , Blood Chemical Analysis , Blood Glucose/analysis , Child , Child, Preschool , Fatty Acids, Nonesterified/blood , Female , Humans , Ketone Bodies/blood , Lactates/blood , Lactic Acid , Male , Pyruvates/blood , Pyruvic Acid , Sex Factors , Time Factors , Triglycerides/bloodABSTRACT
The interrelation between blood components, involved in fuel metabolism, and age, sex and glucose was studied in 72 control children (26 girls and 46 boys, aged between 3 and 15 yr) after an overnight fast (14 h). Glucose, lactate, pyruvate, triglycerides and cholesterol are age-independent. Alanine is positively correlated, whereas beta-hydroxybutyrate, acetoacetate and NEFA's are negatively correlated with age. With respect to blood sugar, acetoacetate, NEFA's and cholesterol are glucose-independent. Lactate, pyruvate, alanine and triglycerides are positively correlated with glucose, and beta-hydroxybutyrate--and total ketone bodies--are negatively correlated with glucose. Except for triglycerides, no differences in the concentrations of the above mentioned blood substrates are seen between boys and girls. These data demonstrate that after an overnight fast lipolysis and ketogenesis already are active in young children, probably related to inadequate gluconeogenesis and improvement of carbohydrate regulation with age.
