ABSTRACT
AIM: Search of association of polymorphisms in DEFB1, IL-10, TNF-α and TLR2 genes with development of chronic generalized periodontitis in representatives of Ural region (Caucasian race). MATERIALS AND METHODS: 142 patients, that were split into 3 groups, took part in the study: a group of patients with periodontitis, a group with frequent inflammatory disease of upper respiratory tract and a comparison group--healthy donors. A study of polymorphic markers was carried out: DEFB1 (-44G/C), DEFB1 (-20A/G), IL-10 (-1082.A/G), TNF-α (-308 G/A), Arg753Gln and Arg677Trp using PCR in real time mode. RESULTS: Association of infectious pathology ofupper respiratory tract and development of periodontitis diseases with markers in DEFB1 (-44G/C) and Arg753Gln and Arg677Trp genes was determined. Significant differences in distribution of genotypes and alleles of genes IL-10 and TNF-α in the group of patients with periodontitis and comparison group were not detected. CONCLUSION: DEFB1 (-44G/C) polymorphism can be examined as a marker of periodontitis development risk.
Subject(s)
Interleukin-10/genetics , Periodontitis/genetics , Respiratory Tract Infections/genetics , Toll-Like Receptor 2/genetics , Tumor Necrosis Factor-alpha/genetics , beta-Defensins/genetics , Adult , Alleles , Biomarkers/metabolism , Case-Control Studies , Chronic Disease , Female , Gene Expression , Gene Frequency , Genetic Predisposition to Disease , Humans , Immunity, Innate , Interleukin-10/immunology , Male , Middle Aged , Periodontitis/complications , Periodontitis/immunology , Periodontitis/pathology , Polymorphism, Single Nucleotide , Promoter Regions, Genetic , Real-Time Polymerase Chain Reaction , Respiratory Tract Infections/complications , Respiratory Tract Infections/immunology , Respiratory Tract Infections/pathology , Toll-Like Receptor 2/immunology , Tumor Necrosis Factor-alpha/immunology , beta-Defensins/immunologyABSTRACT
The most significant of the primary stages of complex therapy of oral lichen planus (OLP), among causal and pathogenetic therapy is a local conservative treatment. The aim of the study was to evaluate the clinical efficacy of the local use of the new compositions TIZOL with triamcinolon in complex therapy of erosive-ulcerous forms OLP oral mucosa. The study was performed with 47 patients with lichen planus in age from 24 to 70 years with erosive-ulcerous form OLP whose diagnosis was confirmed histologically. The first group included 25 patients in the complex treatment of locally applied composition TIZOL with triamcinolon. The second group of 22 people, who in the complex treatment applied locally 0.5% prednisone ointment. The high efficiency of topical TIZOL with a highly topical steroid in the complex therapy of erosive-ulcerous forms OLP, which was confirmed by the positive clinical dynamics in all patients (100%) and high self-esteem of patients (84% positive ratings), reduced life complete epithelialization of erosions.
Subject(s)
Lichen Planus, Oral/drug therapy , Oral Ulcer/drug therapy , Triamcinolone/therapeutic use , Administration, Topical , Adult , Aged , Drug Combinations , Drug Therapy, Combination , Female , Gels , Humans , Lichen Planus, Oral/pathology , Male , Middle Aged , Mouth Mucosa/drug effects , Mouth Mucosa/pathology , Oral Ulcer/pathology , Titanium , Triamcinolone/administration & dosage , Young AdultSubject(s)
Dentists, Women , Dentists, Women/statistics & numerical data , Dentists , Adult , Attitude of Health Personnel , Dentists/psychology , Dentists/statistics & numerical data , Dentists, Women/psychology , Female , Health Care Reform , Humans , Male , Siberia , Sociology , Surveys and QuestionnairesABSTRACT
Lipid peroxidation parameters were assessed from the levels of malonic dialdehyde, antioxidant activity from the blood and salivary total antioxidant activity, biopsy specimens of the minor salivary glands were examined by electron microscopy in 34 patients with Sjogren's disease. Both blood and salivary levels of malonic dialdehyde were found increased whereas total antioxidant activity lowered in these patients; destructive changes were detected in the acinar cells basal membranes. The authors suggest that spontaneous (because of insufficiency of antioxidant enzymic systems) lipid peroxidation reactions are responsible for these changes.
