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1.
Environ Pollut ; 235: 938-947, 2018 Apr.
Article in English | MEDLINE | ID: mdl-29751398

ABSTRACT

Previous studies reported that some species of the family Ceratophryidae are able to produce sounds during premetamorphic tadpole stages. We have now determined the effects of the cholinesterase-inhibiting insecticide chlorpyrifos (CPF) on sounds emitted by tadpoles of Ceratophrys ornata. Tadpoles were exposed individually in order to evaluate the progression of effects. Effects on sound production were complemented with common ecotoxicological endpoints (mortality, behavior, abnormalities and growth inhibition). C. ornata was found to be more sensitive than other native (= 67%, 50%) and non-native species (= 75%, 100%) considering lethal and sublethal endpoints, respectively. Effects on sounds appear along with alterations in swimming, followed by the presence of mild, then severe abnormalities and finally death. Therefore, sound production may be a good biomarker since it anticipates other endpoints that are also affected by CPF. Ceratophrys ornata is a promising new model species in ecotoxicology.


Subject(s)
Anura/physiology , Chlorpyrifos/toxicity , Insecticides/toxicity , Animals , Cholinesterases/metabolism , Ecotoxicology , Larva/drug effects , Swimming
2.
Ecotoxicol Environ Saf ; 147: 471-479, 2018 Jan.
Article in English | MEDLINE | ID: mdl-28903092

ABSTRACT

Acute lethal and sublethal toxicity of the pirimicarb-based commercial formulation Aficida® were evaluated on Boana pulchella tadpoles. Whereas mortality was used as end point for lethality, frequency of micronuclei and other nuclear abnormalities as well as alterations in the frequency of erythroblasts in circulating blood as biomarkers for genotoxicity and cytotoxicity, respectively. Swimming, growth, developmental and morphological abnormalities were also employed as sublethal end points. Results show that the species is within the 13th percentile of the distribution of acute sensitivity of species to pirimicarb for aquatic vertebrates. Results revealed values of 23.78 and 101.45mg/L pirimicarb as LC5096h for GS25 and GS36 tadpoles, respectively. The most evident effects were related with the swimming activity with NOEC and LOEC values within the 0.005-0.39mg/L pirimicarb concentration range. Aficida® induced DNA damage at the chromosomal level by increasing micronuclei frequency and other nuclear abnormalities, i.e., lobbed and notched nuclei and binucleated cells. Cellular cytotoxicity was found after Aficida® treatment. The presence of abdominal oedemas in exposed organisms and thus flotation response of organisms could be proposed as a new sensitive exposure parameter. The multiple end point assessment approach used allowed a complete understanding the multi level of effects occurring by exposure to pirimicarb, at least in B. pulchella.


Subject(s)
Carbamates/toxicity , DNA Damage , Insecticides/toxicity , Larva/drug effects , Pyrimidines/toxicity , Water Pollutants, Chemical/toxicity , Animals , Anura , Dose-Response Relationship, Drug , Larva/genetics , Lethal Dose 50 , Swimming , Toxicity Tests, Acute , Toxicity Tests, Subacute
3.
Environ Monit Assess ; 189(2): 63, 2017 Feb.
Article in English | MEDLINE | ID: mdl-28105564

ABSTRACT

The Argentine stretch of the del Plata basin crosses regions devoted to extensive and intensive agriculture mostly with chemical pest control. The utilization of pesticides in the region has increased 900% in the last two decades associated with the introduction of biotech crops and direct-seeding techniques. Our objective was to study the occurrence, concentration, and fate of pesticides in surface water and bottom sediments of the principal tributaries and main watercourse of the Paraguay-Paraná River. We sampled 22 sites in the distal positions of the main affluents and main watercourse of the Paraná and report here results from two monitoring campaigns (2010-2012). Surface water and bottom sediments were analyzed according to standardized methods by matrix-solid-phase dispersion and liquid-liquid extraction, respectively. Twenty-three pesticide compounds were analyzed by gas chromatography. The results from both campaigns indicated a generalized but variable distribution in the concentrations detected throughout the basin. The ranges of total measured pesticide concentrations in water and sediments were, respectively, 0.004-6.62 µg/l and 0.16-221.3 µg/kg dry weight. Endosulfans, cypermethrin, and chlorpyrifos were ubiquitous compounds in both environmental compartments and quantitatively the most relevant. All concentrations detected in water were over the recommended guidelines for the protection of aquatic biota. The partitioning indicated a higher affinity for the sediments. Agricultural activity is the source of pesticide-pollution loads, transported by tributaries that reach the main watercourse and alter the quality of the aquatic ecosystem.


Subject(s)
Pesticides/analysis , Water Pollutants, Chemical/analysis , Agriculture , Argentina , Chlorpyrifos , Ecosystem , Environmental Monitoring , Geologic Sediments/chemistry , Paraguay , Rivers/chemistry
4.
Environ Monit Assess ; 188(8): 458, 2016 Aug.
Article in English | MEDLINE | ID: mdl-27395359

ABSTRACT

The Paraná River, the sixth largest in the world, is the receptor of pollution loads from tributaries traversing urban and industrialized areas plus agricultural expanses, particularly so in the river's middle and lower reaches along the Argentine sector. In the present study, we analyzed and discussed the main water quality parameters, sediment compositions, and content of the herbicide glyphosate plus its metabolite aminomethylphosphonic acid (AMPA) in water and sediments. Samples were obtained from distal positions in the principal tributaries of the Paraná and the main watercourse during surveys conducted in 2011 and 2012 to monitor the basin. Only 15 % of the water samples contained detectable concentrations of glyphosate at an average concentration of 0.60 µg/L, while no detectable levels of AMPA were observed. The herbicide and metabolite were primarily present in sediments of the middle and lower stretch's tributaries, there occurring at a respective average of 37 and 17 % in samples. The mean detectable concentrations measured were 742 and 521 µg/kg at mean, maximum, and minimum glyphosate/AMPA ratios of 2.76, 7.80, and 0.06, respectively. The detection of both compounds was correlated with the presence of sulfides and copper in the sediment matrix.


Subject(s)
Geologic Sediments/analysis , Glycine/analogs & derivatives , Herbicides/analysis , Organophosphonates/analysis , Water Pollutants, Chemical/analysis , Agriculture , Argentina , Environmental Monitoring , Glycine/analysis , Isoxazoles , Rivers , Tetrazoles , Water Quality , Glyphosate
5.
Bull Environ Contam Toxicol ; 96(5): 591-5, 2016 May.
Article in English | MEDLINE | ID: mdl-27003804

ABSTRACT

Genotoxic effects of Cd(+2), Cr(+6), and Cu(+2) on the gill and liver of the Argentinean Silverside (Odontesthes bonariensis) were studied using the comet assay and in relation with the metal tissue accumulation. Fish were exposed to three waterborne concentrations of each metal for 2 and 16 days. Genotoxicity was assessed by the single cell gel electrophoresis (comet assay). After 2 days, significant increase of the genetic damage index (GDI) was only observed in the gill of fish exposed to Cr(+6) and Cu(+2), and the LOECs were 2160 nM and 921.1 nM, respectively. The gill LOEC for Cd(+2) by 16 days was 9.4 nM. In the liver, LOECs were obtained only for Cd(+2) and Cr(+6) and were 9.4 and 2160 nM, respectively. The three metals were able to induce genotoxic effects at environmentally relevant concentrations and the gill was the most sensitive organ.


Subject(s)
Fishes/physiology , Gills/drug effects , Liver/drug effects , Metals, Heavy/toxicity , Water Pollutants, Chemical/toxicity , Animals , Cadmium/toxicity , Chromium/toxicity , Comet Assay , Copper/toxicity , DNA Damage , Environmental Exposure , Metals , Mutagenicity Tests
6.
Dis Aquat Organ ; 104(2): 163-71, 2013 May 27.
Article in English | MEDLINE | ID: mdl-23709469

ABSTRACT

The occurrence of abnormalities in amphibians has been reported in many populations, and its increase could be related to environmental pollution and habitat degradation. We evaluated the type and prevalence of abnormalities in 5 amphibian populations from agroecosystems with different degrees of agricultural disturbance (cultivated and reference areas). We detected 9 types of abnormalities, of which the most frequent were those occurring in limbs. The observed prevalence of abnormality in assessed populations from cultivated and reference areas was as follows: Rhinella fernandezae (37.1 and 10.2%, respectively), Leptodactylus latrans adults (28.1 and 9.2%) and juveniles (32.9 and 15.3%), and Hypsiboas pulchellus (11.6 and 2.8%). Scinax granulatus populations did not show abnormalities. Pseudis minuta, which was only detected in the reference area, exhibited a prevalence of 13.3%. For R. fernandezae, L. latrans, and H. pulchellus, generalized linear mixed models showed that prevalence of abnormalities was significantly higher (p < 0.05) in cultivated than in reference areas. L. latrans juveniles were more vulnerable to abnormalities than adults (p < 0.05). The presence of abnormalities in some species inhabiting different agroecosystems suggests that environmental stress factors might be responsible for their occurrence. While we detected pesticides (endosulfan, cypermethrin, and chlorpyrifos) and lower dissolved oxygen levels in ponds of the cultivated area, no data are currently available on how other factors, such as injuries from predators and parasite infections, vary by land use. Further research will be necessary to evaluate possible causes of abnormalities detected in the present study mainly in the context of factor interactions.


Subject(s)
Agriculture , Anura , Ecosystem , Extremities/pathology , Animals , Argentina , Environmental Pollutants/chemistry , Environmental Pollutants/toxicity , Pesticides/chemistry
7.
Bull Environ Contam Toxicol ; 79(5): 537-43, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17940715

ABSTRACT

The use of formulations of the herbicide glyphosate in transgenic crops of the Pampa's plains of Argentina has extensively increased, though there is scarce information of its impact on non-target vascular plants from agro-ecosystem related surface waters. The sensitivity of a local clone of the macrophyte Lemna gibba L. to glyphosate active principle and Roundup Max formulation was studied in standardized laboratory conditions. Phytotoxic effects, considering the aquatic route, at a concentration range of glyphosate between 0.5 and 80 mg L(-1) as active ingredient during 10 days of exposure were assessed on plant population growth, frond growth, shape and number, total chlorophyll content and colony architecture. Exposure to 1 mg L(-1) of glyphosate (an expected environmental concentration) affects all the studied assessment endpoints, except for population growth and chlorophyll content. Equivalent concentrations of this herbicide as the active ingredient or RoundupMax indicate higher phytotocity of the formulation. Exposed plants at concentrations of herbicide between 1 and 7.5 mg L(-1) exhibit after two days a recovery of the multiplication rate. Frond aggregation and longer stipe was detected between 1 and 15 mg L(-1) of glyphosate, determining more open colony architecture. At higher concentrations of the herbicide fronds break-up. Comparisons with literature data indicate a higher sensitivity of the L. gibba local clone with respect to L. minor and algal species, and also a similar response to the herbicide in field experiments with the same species.


Subject(s)
Araceae/drug effects , Glycine/analogs & derivatives , Herbicides/pharmacology , Dose-Response Relationship, Drug , Glycine/pharmacology , Water Pollutants, Chemical/pharmacology , Glyphosate
9.
Dis Aquat Organ ; 72(3): 261-7, 2006 Oct 27.
Article in English | MEDLINE | ID: mdl-17190204

ABSTRACT

In the last few years there has been great concern about declines in the abundance of several species of amphibians around the world. Among amphibians, anurans have a biphasic life cycle, with aquatic tadpoles and generally terrestrial adults, and they have an extremely permeable skin, making them excellent indicators of the health of the environment. A number of different causes have been suggested for the global decline of anurans, the pollution of their habitat by chemical stressors being considered one of the major factors. Among chemical stressors, heavy metals are known for their high toxicity at very low concentrations. This study assessed short- (96 h, 'acute') and long-term (1272 h, 'chronic') exposure to Cr(VI) at lethal (3 to 90 mg 1(-1)) and sublethal concentrations (0.001 to 12 mg 1(-1)) on Hypsiboaspulchellus (previously called Hyla pulchella; see Faivovich et al. 2005) tadpoles (Fam. Hylidae) from central eastern Argentina. Fertilized eggs collected from a clean pond near La Plata (Buenos Aires Province) were used for acute and chronic toxicity testing. Assays were done under controlled laboratory conditions. Results of chronic exposure were used to assess the effect of factors such as toxicant concentration and age of organisms at the beginning of exposure on the response variables (growth, development and survival until metamorphosis). Results indicated a higher sensitivity to Cr(VI) of individuals first exposed as tadpoles than those first exposed as embryos during acute and chronic exposure. Exposure to the highest sublethal concentrations (6 to 12 mg 1(-1)) of the toxicant showed early inhibitory effects on growth of all treated organisms compensated at longer exposure periods with an increase in the growth rate compared to the control groups.


Subject(s)
Anura/embryology , Anura/growth & development , Chromium/toxicity , Water Pollutants, Chemical/toxicity , Animals , Embryo, Nonmammalian/drug effects , Larva/drug effects , Survival Analysis , Time Factors
14.
Proc Natl Acad Sci U S A ; 90(3): 1078-82, 1993 Feb 01.
Article in English | MEDLINE | ID: mdl-8430077

ABSTRACT

Nitrogenase (EC 1.18.6.1) catalyzes the conversion of dinitrogen to ammonia, the central reaction of biological nitrogen fixation. X-ray anomalous diffraction data were analyzed to probe the structures of the metal clusters bound by nitrogenase MoFe protein. In addition to one FeMo cofactor, each half-molecule of MoFe protein binds one large FeS cluster of a type not previously observed in a protein. The FeS cluster contains roughly eight Fe atoms, comprises two subclusters, and is separated from the FeMo cofactor by an edge-to-edge distance of 14 A. The inorganic framework of the FeMo cofactor is not resolved into subclusters, but the Mo atom is located at its periphery. FeMo cofactors in different half-molecules are 70 A apart and cannot promote binuclear activation of dinitrogen by two Mo atoms.


Subject(s)
Clostridium/enzymology , Molybdoferredoxin/chemistry , Iron/chemistry , Molybdenum/chemistry , Protein Conformation , Scattering, Radiation , Sulfur/chemistry , X-Ray Diffraction/methods
15.
Environ Pollut ; 82(3): 263-7, 1993.
Article in English | MEDLINE | ID: mdl-15091775

ABSTRACT

Natural fulvic acids (FA) from the Rio Santiago, an affluent of the Rio de la Plata estuary, were isolated by adsorption on XAD-2 at pH 2, and were then characterized by IR and fluorescence spectra, acidity and complexing capacity to Cu(II). The effect of the interaction between FA and Cu(II) on the acute toxicity of this heavy metal was assayed with the rotifer Brachionus calyciflorus in standardized in-vitro tests. A CuSO(4).5H(2)O concentration of 0.080 ppm produced 89.2% mortality in the absence of FA. At FA/Cu ratios >/= 1 no mortality was observed. Based on the values of the FA-Cu binding parameters and acute toxicity inhibition, it was possible to predict the effect of dissolved organic matter on the toxicity of copper in this surface freshwater body.

16.
World J Microbiol Biotechnol ; 8(3): 316-8, 1992 May.
Article in English | MEDLINE | ID: mdl-24425487

ABSTRACT

Lyophilized cells ofPhotobacterium phosphoreum, rehydrated in 2% (w/v) NaCl in 0.022M KH2PO4 at pH 7.0, were used for developing an assay to test the acute toxicity of organic and inorganic compounds. The standardized assay gave good reproducibility of results with 11 organic and four inorganic compounds. Results were compared with reported data obtained with other test organisms and are within their sensitivity ranges. Environmental screening of wastes from oil and petrochemical industries is discussed.

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