Expression level of Th22 cells and its cytokines in patients with acute lymphoblastic leukemia and its significance / 中国实验血液学杂志

This study was purposed to analyze the expression level of Th22 cells and their cytokines in patients with acute lymphoblastic leukemia (ALL) and evaluate its significance. Forty-eight patients with ALL were selected. According to the treatment, all patients were divided into the newly diagnosed group (n = 26) and complete remission (CR) group (n = 22). The proportion of Th22 cells in peripheral blood was detected by flow cytometry (FCM). The expression levels of cytokines IL-22, IL-6, TNF-α and TGF-β in peripheral blood were measured by ELISA. The expression level of IL-22 mRNA in peripheral blood mononuclear cells was examined by semi-quantitative-reverse transcription PCR (RT-PCR). Meanwhile, 30 healthy individuals were selected as a control group. The parameters of the 3 groups were compared. The results showed that the percentage of Th22 cells and the expression levels of IL-22, IL-6, TNF-α and IL-22 mRNA in newly diagnosed group and the CR group were significantly lower than that in control group, the expression level of TGF-β in above mentioned two group was obviously higher than that in control group (P < 0.05). The percentage of Th22 cells and the expression levels of IL-22, IL-6, TNF-α and IL-22 mRNA in newly diagnosed group were evidently lower than that in CR group (P < 0.05), but the expression level of TGF-β in newly diagnosed group obviously higher than that in CR group. The expression level of IL-22 in newly diagnosed group was positively related with expression level of IL-6 and TNF-α, but it was negatively related with expression level of TGF-β. It is concluded that the decreasing of Th22 cells and down-regulation of IL-22 expression level may be related with pathogenesis of ALL, the decreasing of Th22 cells is risk factor for ALL.

Endovascular management strategy for patients with acute occlusive internal carotid artery dissection combined with tandem middle cerebral artery occlusion / 中华神经医学杂志

Objective To investigate the endovascular management strategy for patients with acute occlusive internal carotid artery (ICA) dissection combined with tandem middle cerebral artery (MCA) occlusion,and assess its safety and outcomes.Methods Six ICA dissection patients with tandem middle cerebral artery occlusion,admitted to our hospital from March 2011 to December 2012,were treated with endovascular treatment.Recanalization of ICA dissection and Solitaire AB stent thrombectomy for MCA occlusion were achieved in acute stage.Endovascular stent therapy for ICA dissection was performed in stable phase.The clinical data and the results of treatment of these patients were retrospectively analyzed.Results Successful recanalization of MCA was obtained in all 6 patients; one ICA dissection was recanalized by Solitaire AB stent (6 mm×20 mm) and four ICA dissections were recanalized by 6F guiding catheter introduced by microcatheter; in the other one patient with tortuosity of ICA,recanalization of dissection was failed but recanalizztion was achieved one month after anticoagulation and antiplatelet; five patients accepted successful endovascular stent therapy for ICA dissection in the second phase.The mean National Institutes of Health Stroke Scales (NIHSS) scores were four points two weeks after procedure,which showed obvious increase as compared with those before procedure (16 points).Angiography showed MCA was patent after six months in two patients and dissection recovered completely by stent.All patients got follow-up in the Out-patient Department and no patient suffered a new ischemic stroke.Five patients had good mRS scores (≤ 2) with only one reaching 3 points.Conclusion Solitaire stent thrombectomy for MCA occlusion in acute stage,followed by endovascular stent therapy for ICA dissection in stable phase,may be an effective,safe and attractive treatment for tandem MCA occlusion combined with ICA dissection.