ABSTRACT
Objective@#To investigate the expression of long non-coding RNA (lncRNA)H19 in gallbladder cancer and its relationship with clinicopathological parameters and prognosis.@*Methods@#74 cases of allbladder cancer resected in our hospital were collected and studied. At the same time, 66 cases of benign gallbladder lesions were selected as benign lesion group. The expression of lncRNA H19 in tissues was detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). The correlation between lncRNA H19 expression and clinicopathological parameters of gallbladder cancer patients was analyzed.The patients were followed up for 3 years and COX was used to analyze the prognostic factors affecting the survival rate.The survival was analyzed by Kaplan-Meier curve. The survival differences of lncRNA H19 expression groups were detected by Log-Rank method.@*Results@#The expression level of lncRNA H19 in gallbladder cancer was significantly higher than that in benign lesions (P<0.05). The expression of lncRNA H19 was correlated with stage, differentiation and lymph node metastasis (P<0.05). Age, sex, histological grade, stage and depth of invasion of gallbladder cancer had no significant correlation with 3-year survival rate (P>0.05). The survival rate of patients with differentiated tumors and non-metastatic lymph nodes were higher (P<0.05). The survival rate of patients with low expression of lncRNA H19 was significantly higher than those with high expression of lncRNA H19 (P<0.05). Tumor differentiation, lymph node metastasis and lncRNA H19 expression were independent risk factors affecting the prognosis of patients with gallbladder cancer.@*Conclusions@#LncRNA H19 is highly expressed in gallbladder cancer, which is closely related to the degree of differentiation, lymph node metastasis and survival rate of patients.
ABSTRACT
Objective To investigate the expression of long non-coding RNA (lncRNA)H19 in gallbladder cancer and its relationship with clinicopathological parameters and prognosis.Methods 74 cases of allbladder cancer resected in our hospital were collected and studied.At the same time,66 cases of benign gallbladder lesions were selected as benign lesion group.The expression of lncRNA H19 in tissues was detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR).The correlation between lncRNA H19 expression and clinicopathological parameters of gallbladder cancer patients was analyzed.The patients were followed up for 3 years and COX was used to analyze the prognostic factors affecting the survival rate.The survival was analyzed by Kaplan-Meier curve.The survival differences of lncRNA H19 expression groups were detected by Log-Rank method.Results The expression level of lncRNA H19 in gallbladder cancer was significantly higher than that in benign lesions (P ＜ 0.05).The expression of lncRNA H19 was correlated with stage,differentiation and lymph node metastasis (P ＜ 0.05).Age,sex,histological grade,stage and depth of invasion of gallbladder cancer had no significant correlation with 3-year survival rate (P ＞ 0.05).The survival rate of patients with differentiated tumors and non-metastatic lymph nodes were higher (P ＜ 0.05).The survival rate of patients with low expression of lncRNA H19 was significantly higher than those with high expression of lncRNA H19 (P ＜ 0.05).Tumor differentiation,lymph node metastasis and lncRNA H19 expression were independent risk factors affecting the prognosis of patients with gallbladder cancer.Conclusions LncRNA H19 is highly expressed in gallbladder cancer,which is closely related to the degree of differentiation,lymph node metastasis and survival rate of patients.
ABSTRACT
Purpose To investigate the presence of perineural invasion (PNI) and the expression of human epidermal growth factor receptor 2 (HER-2) in patients with advanced gastric cancer.To discuss the correlation between PNI and the expression of HER-2 and their prognostic significance.Methods Paraffin sections of surgical specimens from 100 patients who underwent gastric resection were selected,and then PNI were detected by HE staining and the expression of HER-2 were detected by immunohistochemical (IHC).The correlation of perineural invasion with the clinicopathological features and HER-2 expression were performed.Results PNI was positive in 42 cases of 100 patients (PNI positive rate was 42％),the occurrence of PNI has significantly related with lymph node metastases,infiltrating depth,tumor emboli,TNM stage and expression of HER2.The difference was statistically significant (P ＜ 0.05).Univariate analysis showed that total survival rate of advanced gastric cancer was associated with T stage,tumor emboli,N stage,TNM stage,PNI and HER-2 expression (P ＜ 0.05).The overall survival of the PNI-negative patients was 45.3 months on average,much better than the PNI positive patients who was 15.4months in average (P ＜ 0.05).The overall survival of the HER2-negative patients was 43.2 months on average,much better than the HER-2-positive patients who was 22.7 months in average (P ＜ 0.05).By muhivariable Cox proportional hazards model of overall smvival,the positivity of PNI and HER-2 appeared to be new independent prognostic factors (P ＜ 0.05).Conclusion The detection of PNI and HER-2 expression maybe helpful for the prognosis of patients with advanced gastric cancer.In addition,HER-2 may be responsible for the presence of perineural invasion.
