Determination of paprazine in Polygonum orientale of different commodities by HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a method for the content determination of paprazine in Polygonum orientale, and provide a new evaluation index for the quality control for P. orientale.</p><p><b>METHOD</b>HPLC was carried out on Agilent C18 (4.6 mm x 150 mm, 5 microm) with methanol-water (40:60) as mobile phase. The flow rate was 1.0 mL x min(-1) and the wavelength of UV detector was set at 290 nm.</p><p><b>RESULT</b>The calibration curve was linear in the range of 0. 038-0.190 microg (r = 0.9999). The average recovery was 98.7% with the RSD of 2.36%.</p><p><b>CONCLUSION</b>The method appears to be simple, accurate and reproducible, it can be used to determine the content of paprazine in P. orientale.</p>

Determination of eight kinds of ginsenosides in Shizhu ginseng / 中国中药杂志

<p><b>OBJECTIVE</b>To establish the method for determining simultaneously eight kinds of ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rc, Rb3 and Rd and their contents in Shizhu ginseng.</p><p><b>METHOD</b>HPLC was carried out on Agilent TC-C18 (4.6 mm x 150 mm, 5 microm) with acetonitrile -0.05% phosphoric acid, gradient elution, the volume of flow 1 mL x min(-1), column temperature 30 degrees C and detection wavelength 203 nm.</p><p><b>RESULT</b>The contents of eight kinds of ginsenosides in Shizhu ginseng were 1.32, 2.08, 0.72, 0. 24, 2.12, 1.06, 0.35, 0.55 mg x g(-1), respectively.</p><p><b>CONCLUSION</b>The contents of ginsenoside ingredients in Shizhu ginseng were determined in the first time. The established method can be used in determining the contents of multiple gensenosides in herb ginseng simultaneously.</p>