ABSTRACT
BACKGROUND: The roles of FTO gene and the level of serum 25-OH-vitamin D in obesity are frequently reported. This study aimed to investigate the interactions of serum 25-OH-vitamin D level, FTO and IRX3 genes expression, and FTO genotype in obese and overweight boys. METHODS: This study was carried out on the 120 male adolescents with overweight in Tehran, Iran. Blood samples were collected from the participants in order to evaluate the serum level of 25-OH-vitamin D, the expression level of FTO and IRX3 genes, and FTO genotype for rs9930506 at baseline and after 18 weeks of the study. RESULTS: In general, no significant association was found between serum 25-OH-vitamin D level and IRX3 and FTO genes expression. The results of linear regression on the relationship between 25-OH-vitamin D serum level and FTO and IRX3 genes expression based on FTO genotypes for rs9930506 indicated that in AA/AG genotype carriers, serum 25-OH-vitamin D level was positively associated with FTO gene expression (B = 0.07, p = 0.02) and inversely associated with IRX3 gene expression (B = - 0.07, p = 0.03). In GG carriers, serum 25-OH-vitamin D level was not associated with expression of IRX3 and FTO genes. CONCLUSION: There are significant interactions between 25-OH-vitamin D and the expression of FTO and IRX3 genes in the subset of obese patients with specific genotypes for FTO rs9930506. There was no association between serum 25-OH-vitamin D levels and the expression of FTO and IRX genes in individuals with a homozygous genotype for the risk allele of the FTO gene polymorphism.
Subject(s)
Alpha-Ketoglutarate-Dependent Dioxygenase FTO , Polymorphism, Single Nucleotide , Adolescent , Alpha-Ketoglutarate-Dependent Dioxygenase FTO/genetics , Gene Expression , Genotype , Homeodomain Proteins/genetics , Humans , Iran , Male , Obesity/genetics , Overweight , Polymorphism, Single Nucleotide/genetics , Transcription Factors/genetics , Vitamin DABSTRACT
In this research heavy metals, Cd and Pb, adsorption efficiency was evaluated in aqueous solutions using live and dead biomass of Pseudomonas aeruginosa bacteria. The various important parameters including; pH, temperature, Cd and Pb concentrations, contact time, live and dead cell mass were examined. First, the resistant P. aeruginosa to heavy metals identified and isolated from contaminated soil. Then, the Minimum Inhibitory Concentration (MIC) of Cd and Pb was determined for P. aeruginosa. The highest adsorption efficiency for Cd and Pb were 87% and 98.5%, under dead cell mass of 125â¯mg, pH 7, temperature 35⯰C and contact time 90â¯min, respectively. The results of this study showed that P. aeruginosa have a high ability to adsorption of Cd and Pb in aqueous solutions.
ABSTRACT
Data on this paper show the concentrations of COD, BOD5, TSS, K+, Ca2+, Na+, Cl-, [Formula: see text], [Formula: see text], Mn2+, Fe2+, Mg2+, Zn2+, Ni, Pb, Cu and Cd in the influent and effluent of wastewater, and also the nematode eggs, total and fecal coliform in effluents from wastewater treatment plant of Rasht, Guilan Province, in Iran. Measurements of pollutants in influent and effluent was measured according to standard methods (W.E. Federation and Association, A. P. H., 2005) [1]. Statistical analysis of the data was carried out using Special Package for Social Sciences (SPSS 16).
