ABSTRACT
A rapid, sensitive and specific high-performance liquid chromatographic method for the quantification of acrolein (1), one of the toxic metabolites of oxazaphosphorine alkylating agents (cyclophosphamide and ifosfamide) was developed. Condensation of acrolein with Luminarin 3 afforded a fluorescent derivative that could be specifically detected and quantified. Chromatographic conditions involved a C18 RP column Uptisphere and a gradient elution system to optimize resolution and time analysis. The method showed high sensitivity with a limit of detection of 100 pmol/ml and a limit of quantification of 300 pmol/ml. This technique is particularly suitable for pharmacokinetic studies on plasma of oxazaphosphorine-receiving patients.
Subject(s)
Acrolein/blood , Chromatography, High Pressure Liquid/methods , Fluorescent Dyes/chemistry , Quinolizines/chemistry , Humans , Magnetic Resonance Spectroscopy , Mass Spectrometry , Reproducibility of Results , Sensitivity and Specificity , Spectrometry, FluorescenceABSTRACT
During batch cultivation of Streptomyces lactamdurans a diauxic growth has been shown taking into consideration dry cell mass, protein, and DNA determination. This phenomenon was connected with substantial changes in environmental conditions such as concentrations of ammonia and amino nitrogen, soluble inorganic phosphate and pH. The antibiotic production was preceded by the preparatory phase occurring at the beginning of the second growth phase. A hypothetical pattern of cell physiology in the curse of cephamycin fermentation is discussed. An essential part in the biphasic cell growth and the induction of antibiotic synthesis in this model is played by phosphate depletion in the medium.
