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1.
J Immunol ; 164(9): 4790-6, 2000 May 01.
Article in English | MEDLINE | ID: mdl-10779786

ABSTRACT

Both the CD4-CD8- (double negative) and CD4-CD8+ T cell lineages have been shown to contain T cells which recognize microbial lipid and glycolipid Ags in the context of human CD1 molecules. To determine whether T cells expressing the CD4 coreceptor could recognize Ag in the context of CD1, we derived CD4+ T cell lines from the lesions of leprosy patients. We identified three CD4+ Mycobacterium leprae-reactive, CD1-restricted T cell lines: two CD1b restricted and one CD1c restricted. These T cell lines recognize mycobacterial Ags, one of which has not been previously described for CD1-restricted T cells. The response of CD4+ CD1-restricted T cells, unlike MHC class II-restricted T cells, was not inhibited by anti-CD4 mAb, suggesting that the CD4 coreceptor does not impact positive or negative selection of CD1-restricted T cells. The CD4+ CD1-restricted T cell lines produced IFN-gamma and GM-CSF, the Th1 pattern of cytokines required for cell-mediated immunity against intracellular pathogens, but no detectable IL-4. The existence of CD4+ CD1-restricted T cells that produce a Th1 cytokine pattern suggests a contributory role in immunity to mycobacterial infection.


Subject(s)
Antigens, CD1/immunology , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/microbiology , Leprosy/immunology , Mycobacterium leprae/immunology , Proteins , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/microbiology , Antigen Presentation , Antigens/biosynthesis , Antigens, Bacterial/immunology , Antigens, Bacterial/metabolism , Antigens, CD1/metabolism , Antigens, Surface , CD4 Antigens/immunology , CD4 Antigens/metabolism , CD4-Positive T-Lymphocytes/metabolism , CD4-Positive T-Lymphocytes/pathology , Cell Membrane/immunology , Cell Membrane/metabolism , Cells, Cultured , Glycolipids/immunology , Glycolipids/metabolism , Humans , Lectins, C-Type , Leprosy/pathology , Lipopolysaccharides/immunology , Lipopolysaccharides/metabolism , Mycolic Acids/immunology , Mycolic Acids/metabolism , NK Cell Lectin-Like Receptor Subfamily B , Peptides/immunology , Peptides/metabolism , Protein Biosynthesis , Receptors, Immunologic/biosynthesis , T-Lymphocyte Subsets/metabolism , T-Lymphocyte Subsets/pathology
2.
Nature ; 404(6780): 884-8, 2000 Apr 20.
Article in English | MEDLINE | ID: mdl-10786796

ABSTRACT

The discovery of the CD1 antigen presentation pathway has expanded the spectrum of T-cell antigens to include lipids, but the range of natural lipid antigens and functions of CD1-restricted T cells in vivo remain poorly understood. Here we show that the T-cell antigen receptor and the CD1c protein mediate recognition of an evolutionarily conserved family of isoprenoid glycolipids whose members include essential components of protein glycosylation and cell-wall synthesis pathways. A CD1c-restricted, mycobacteria-specific T-cell line recognized two previously unknown mycobacterial hexosyl-1-phosphoisoprenoids and structurally related mannosyl-beta1-phosphodolichols. Responses to mannosyl-beta1-phosphodolichols were common among CD1c-restricted T-cell lines and peripheral blood T lymphocytes of human subjects recently infected with M. tuberculosis, but were not seen in naive control subjects. These results define a new class of broadly distributed lipid antigens presented by the CD1 system during infection in vivo and suggest an immune mechanism for recognition of senescent or transformed cells that are known to have altered dolichol lipids.


Subject(s)
Antigens, CD1/immunology , Glycolipids/immunology , Polyisoprenyl Phosphates/immunology , T-Lymphocytes/immunology , Adult , Antibodies, Monoclonal/immunology , Cell Line , Dolichol Monophosphate Mannose/immunology , Female , Glycosylation , Humans , Male , Mycobacterium avium/immunology , Mycobacterium tuberculosis/immunology , Tuberculosis/immunology
4.
J Exp Med ; 189(1): 195-205, 1999 Jan 04.
Article in English | MEDLINE | ID: mdl-9874576

ABSTRACT

The T cell antigen receptor (TCR) mediates recognition of peptide antigens bound in the groove of major histocompatibility complex (MHC) molecules. This dual recognition is mediated by the complementarity-determining residue (CDR) loops of the alpha and beta chains of a single TCR which contact exposed residues of the peptide antigen and amino acids along the MHC alpha helices. The recent description of T cells that recognize hydrophobic microbial lipid antigens has challenged immunologists to explain, in molecular terms, the nature of this interaction. Structural studies on the murine CD1d1 molecule revealed an electrostatically neutral putative antigen-binding groove beneath the CD1 alpha helices. Here, we demonstrate that alpha/beta TCRs, when transferred into TCR-deficient recipient cells, confer specificity for both the foreign lipid antigen and CD1 isoform. Sequence analysis of a panel of CD1-restricted, lipid-specific TCRs reveals the incorporation of template-independent N nucleotides that encode diverse sequences and frequent charged basic residues at the V(D)J junctions. These sequences permit a model for recognition in which the TCR CDR3 loops containing charged residues project between the CD1 alpha helices, contacting the lipid antigen hydrophilic head moieties as well as adjacent CD1 residues in a manner that explains antigen specificity and CD1 restriction.


Subject(s)
Antigens/immunology , Lipids/immunology , Receptors, Antigen, T-Cell/immunology , T-Lymphocytes/immunology , Animals , Antigens, CD1/chemistry , Antigens, CD1/immunology , Clone Cells/immunology , Cloning, Molecular , Humans , Mice , Models, Molecular , Polymerase Chain Reaction , Protein Structure, Secondary , Transfection/genetics
5.
J Immunol ; 162(1): 366-71, 1999 Jan 01.
Article in English | MEDLINE | ID: mdl-9886408

ABSTRACT

It is generally accepted that TCR alphabeta+ CD8+ T cells recognize immunogenic peptides bound to MHC-encoded class I molecules. This recognition is a major component of the cellular response mediating immune protection and recovery from viral infections and from certain intracellular bacterial infections. Here, we report two human CD8+ TCR alphabeta+ T cell lines specific for Mycobacterium tuberculosis Ags presented in the context of CD1a or CD1c Ag-presenting molecules. These T cells recognize lipid Ags and display cytotoxicity as well as strong Th cell type I cytokine responses. By extending presentation by the CD1 system to the major TCR alphabeta+ CD8+ T cell pool, this system gains wider applicability beyond the double negative subset of T cells previously shown to have this reactivity. This implies that previous assumptions about the role of CD8+ T cells in microbial immunity may require revision as the relative proportions of CD1-restricted and MHC class I-restricted CD8+ T cells are further defined.


Subject(s)
Antigens, Bacterial/immunology , Antigens, CD1/physiology , CD8-Positive T-Lymphocytes/metabolism , Epitopes, T-Lymphocyte/immunology , Lipids/immunology , Receptors, Antigen, T-Cell, alpha-beta/immunology , T-Lymphocyte Subsets/metabolism , Antibodies, Blocking/pharmacology , Antibodies, Monoclonal/pharmacology , CD8-Positive T-Lymphocytes/immunology , Cell Line , Chromatography, Gel , Cytokines/biosynthesis , Cytotoxicity Tests, Immunologic , Glycolipids/immunology , Glycolipids/isolation & purification , Humans , Lipids/isolation & purification , Lymphocyte Activation , Mycobacterium tuberculosis/immunology , Phospholipids/immunology , Phospholipids/isolation & purification , T-Lymphocyte Subsets/immunology
6.
Clin Immunol Immunopathol ; 87(1): 8-14, 1998 Apr.
Article in English | MEDLINE | ID: mdl-9576005

ABSTRACT

Despite identification of the CD1 family of molecules in the late 1970s, the function of CD1 was undetermined for more than a decade. Recent evidence has established that CD1 molecules comprise a novel lineage of antigen-presenting molecules, distinct from major histocompatibility complex (MHC) class I and class II molecules. Unlike the MHC molecules, which bind short peptides in their antigen-binding groove for presentation to either CD4+ or CD8+ T cells bearing alpha beta T cell receptors, the CD1 molecules appear to accommodate lipid and glycolipid antigens in their hydrophobic cavity for presentation to a wide variety of T cells, including double-negative alpha beta and gamma delta T cells and CD8+ alpha beta T cells. By using a unique cytoplasmic signal, some CD1 molecules traffic to endosomal compartments for sampling mycobacteria-derived lipid antigens, and subsequently lipid antigen-loaded CD1 molecules are expressed on the cell surface to activate specific T cells. These CD1-restricted T cells kill mycobacteria-infected cells and secrete interferon-gamma, indicating a potential role of CD1-mediated T cell responses in clearing mycobacterial infection. The identification of an MHC-independent antigen presentation pathway for nonpeptide antigens provides new insights into immunoregulation and host defense.


Subject(s)
Antigen Presentation , Antigen-Presenting Cells/immunology , Antigens, CD1/physiology , Lymphocyte Activation , T-Lymphocytes/immunology , Animals , Antigens, Bacterial/immunology , Endocytosis , Histocompatibility Antigens Class II/immunology , Humans , Lipids/immunology , Mycobacterium/immunology
7.
Eur J Immunol ; 28(1): 104-13, 1998 Jan.
Article in English | MEDLINE | ID: mdl-9485190

ABSTRACT

Two subpopulations of human T lymphocytes expressing different antigen receptors, alpha/beta and gamma/delta, emigrate into inflamed tissues in distinctive patterns. We compared the transmigration of alpha/beta and gamma/delta T cells to C-C and C-X-C chemokines using an in vitro transendothelial chemotaxis assay. The C-C chemokines monocyte chemoattractant protein (MCP)-1, RANTES, macrophage inflammatory protein (MIP)-1alpha and MIP-1beta stimulated similar, dose-dependent chemotaxis of purified gamma/delta T cells, whereas MCP-1, RANTES, and MIP-1alpha produced greater chemotaxis of purified alpha/beta T cells than MIP-1beta. In contrast, the C-X-C chemokines interleukin (IL)-8 and interferon-gamma inducible protein-10 (IP-10) did not promote chemotaxis of either alpha/beta or gamma/delta T cells. Three gamma/delta T cell clones with differing CD4 and CD8 phenotypes also migrated exclusively to C-C chemokines. Phenotypic analysis of mononuclear cells that transmigrated from an input population of unfractionated peripheral blood mononuclear cells confirmed the results with purified gamma/delta T cells. Our data demonstrate that human peripheral blood alpha/beta and gamma/delta T cells can transmigrate to MCP-1, RANTES, MIP-1alpha, and MIP-1beta, and suggest that both T lymphocyte subpopulations share the capacity to emigrate in response to C-C chemokines during inflammation.


Subject(s)
Chemokines, CXC , Chemotactic Factors/pharmacology , Chemotaxis, Leukocyte/drug effects , Macrophage Inflammatory Proteins/pharmacology , Receptors, Antigen, T-Cell, alpha-beta/analysis , Receptors, Antigen, T-Cell, gamma-delta/analysis , T-Lymphocyte Subsets/drug effects , Chemokine CCL2/pharmacology , Chemokine CCL3 , Chemokine CCL4 , Chemokine CCL5/pharmacology , Chemokine CXCL10 , Chemokines/pharmacology , Dose-Response Relationship, Immunologic , Endothelium, Vascular , Humans , Inflammation , Recombinant Proteins/pharmacology , T-Lymphocyte Subsets/cytology
8.
Science ; 276(5319): 1684-7, 1997 Jun 13.
Article in English | MEDLINE | ID: mdl-9180075

ABSTRACT

In analyzing mechanisms of protection against intracellular infections, a series of human CD1-restricted T cell lines of two distinct phenotypes were derived. Both CD4(-)CD8(-) (double-negative) T cells and CD8(+) T cells efficiently lysed macrophages infected with Mycobacterium tuberculosis. The cytotoxicity of CD4(-)CD8(-) T cells was mediated by Fas-FasL interaction and had no effect on the viability of the mycobacteria. The CD8(+) T cells lysed infected macrophages by a Fas-independent, granule-dependent mechanism that resulted in killing of bacteria. These data indicate that two phenotypically distinct subsets of human cytolytic T lymphocytes use different mechanisms to kill infected cells and contribute in different ways to host defense against intracellular infection.


Subject(s)
Antigens, CD1/immunology , Cytotoxicity, Immunologic , Macrophages/immunology , Mycobacterium tuberculosis/immunology , T-Lymphocyte Subsets/immunology , T-Lymphocytes, Cytotoxic/immunology , Cell Line , Coculture Techniques , Colony Count, Microbial , Cytoplasmic Granules/immunology , Fas Ligand Protein , Granzymes , Humans , Lymphocyte Activation , Macrophages/microbiology , Membrane Glycoproteins/genetics , Membrane Glycoproteins/immunology , Membrane Glycoproteins/metabolism , Mycobacterium tuberculosis/growth & development , Perforin , Phenotype , Pore Forming Cytotoxic Proteins , Serine Endopeptidases/metabolism , Strontium/pharmacology , fas Receptor/immunology , fas Receptor/metabolism
9.
J Immunol ; 157(7): 2795-803, 1996 Oct 01.
Article in English | MEDLINE | ID: mdl-8816382

ABSTRACT

Previous studies suggest that CD1 is a family of Ag-presenting molecules distantly related to those encoded by the MHC. However, of the four known human CD1 proteins, only CD1b has been shown to restrict Ag-specific T cell responses. In this study, we have shown that a second member of the human CD1 family, CD1c, could also mediate Ag presentation to T cells. Three T cell lines recognizing mycobacterial Ags in a CD1c-restricted manner were isolated from normal donor blood. These T cells were MHC unrestricted, and their recognition of Ag was independent of the products of the transporter associated with Ag presentation-1/2 and DMA/B genes that are generally required for Ag presentation by MHC-encoded Ag-presenting molecules. Furthermore, unlike MHC-restricted responses to peptides, the CD1c-restricted T cell lines recognized protease-resistant mycobacterial lipid Ags. These T cell lines also showed significant cytotoxicity toward CD1c-expressing target cells even in the absence of mycobacterial Ags, which was shown by clonal analysis to be mediated by a subpopulation of T cells directly reactive to CD1c molecules. Our findings establish the ability of a second member of the CD1 family to restrict responses of Ag-specific T cells, and thus support the general hypothesis that the CD1 family comprises a third lineage of Ag-presenting molecules that presents a novel class of foreign and self Ags to MHC-unrestricted T cells.


Subject(s)
Antigen Presentation , Antigens, CD1/immunology , Mycobacterium tuberculosis/immunology , T-Lymphocyte Subsets/immunology , Antigens, Bacterial/immunology , Antigens, CD1/classification , Cell Line , Humans , Lipopolysaccharides/immunology , Mannosides/immunology , Membrane Glycoproteins/immunology , Phosphatidylinositols/immunology
10.
J Exp Med ; 183(3): 1193-203, 1996 Mar 01.
Article in English | MEDLINE | ID: mdl-8642261

ABSTRACT

We have compared the ability of human alpha/beta and gamma/delta T lymphocytes to adhere to selectin-bearing substrates, an interaction thought to be essential for homing and localization at sites of inflammation. Both T cell populations form rolling adhesions on E- and P-selectin substrates under physiologic flow conditions. Although equivalent to alpha/beta T cells in binding to E-selectin, gamma/delta T cells demonstrated greater ability to adhere to P-selectin that was purified or expressed on the surface of activated, adherent platelets. Under static conditions, 80% of gamma/delta T cells and 53% of alpha/beta T cells formed shear-resistant adhesions to P-selectin, whereas only 30% of gamma/delta and alpha/beta T cells adhered to E-selectin. The enhance ability of gamma/delta T cells to adhere to P-selectin cannot be attributed to differences in expression of the P-selectin glycoprotein ligand (PSGL-1), as all alpha/beta T cells versus approximately 75% of gamma/delta T cells expressed PSGL-1. Both cell populations expressed a similar percentage of the carbohydrate antigens sialyl LewisX and cutaneous lymphocyte-associated antigen. Depletion of lymphocyte populations or T cell clones bearing these oligosaccharides with the monoclonal antibody CSLEX-1 and HECA-452, respectively, resulted in a substantial reduction in adhesion to E-selectin and slight reduction in adhesion to P-selectin under flow conditions. Treatment of cells with an endopeptidase that selectively degrades O-sialomucins such as PSGL-1, abolished P-selectin but not E-selectin adhesion. Removal of terminal sialic acids with neuraminidase or protease treatment of cells abrogated cell adhesion to both selectin substrates. These results provide direct evidence for the presence of distinct E- and P-selectin ligands on T lymphocytes and suggest that gamma/delta T cells may be preferentially recruited to inflammatory sites during the early stages of an immune response when P-selectin is upregulated.


Subject(s)
Blood Platelets/physiology , Cell Adhesion , E-Selectin , P-Selectin , Receptors, Antigen, T-Cell, alpha-beta/physiology , Receptors, Antigen, T-Cell, gamma-delta/physiology , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/physiology , Antigens, CD/immunology , CD3 Complex/immunology , Cell Separation , Cells, Cultured , E-Selectin/physiology , Flow Cytometry , HL-60 Cells , Humans , Lipid Bilayers , Lymphocyte Depletion , Neutrophils/cytology , Neutrophils/physiology , P-Selectin/physiology , Platelet Adhesiveness , Receptor-CD3 Complex, Antigen, T-Cell/immunology , Stress, Mechanical , T-Lymphocyte Subsets/cytology
11.
Infect Immun ; 63(8): 3000-4, 1995 Aug.
Article in English | MEDLINE | ID: mdl-7622222

ABSTRACT

T cells belong to either the alpha beta+ or gamma delta+ lineage as defined by their antigen receptor. Although both T-cell subsets have been shown to be involved in the immune response to the parasite Leishmania major, very little is known about possible interactions between these two populations. In this study, using a mouse model of infection with L. major, we showed that expansion of a subset of gamma delta+ T cells in vivo is dependent upon the presence of alpha beta+ CD4+ T cells. Moreover, this effect appears to be mediated via the secretion of lymphokines by CD4+ cells with a T-helper 2 (Th2) functional phenotype. Results showing that activation of Th2-type cells in mice treated with anti-immunoglobulin D antibodies or infected with Nippostrongylus brasiliensis also results in gamma delta+ T-cell expansion suggest that this effect of the Th2-type CD4+ cells is a general phenomenon not restricted to infection with L. major.


Subject(s)
Leishmania major/immunology , Leishmaniasis, Cutaneous/immunology , Receptors, Antigen, T-Cell, gamma-delta/immunology , T-Lymphocyte Subsets/immunology , Th2 Cells/immunology , Animals , CD8-Positive T-Lymphocytes/immunology , Lymphocyte Activation , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Inbred CBA , Mice, Nude , Nippostrongylus/immunology , Strongylida Infections/immunology , Th1 Cells/immunology
12.
Eur J Immunol ; 24(2): 496-9, 1994 Feb.
Article in English | MEDLINE | ID: mdl-8299701

ABSTRACT

Previous work from this laboratory has revealed that infection of mice with Leishmania major leads to an expansion of gamma delta+ T cells in the spleen. Further examination of the gamma delta+ T cells expanding in infected mice has shown that the majority of these cells in the spleen, lymph nodes, blood and liver expressed the V delta 4 gene segment. Cell cycle analysis, using propidium iodide incorporation, demonstrated that while only 1% of alpha beta+ T cells in the spleen were in either S + G2/M phase, up to 10% of the gamma delta+ T cells were in cycling phase 8 weeks after infection. Comparison of the state of activation of the two populations in different organs after infection, confirmed that gamma delta+ T cells are actively dividing in lymph nodes, liver and blood, but not in the thymus or among intraepithelial lymphocytes. Examination of the expression of different activation markers on the surface of gamma delta+ T cells in the spleen of both normal and chronically infected BALB/c mice by FACS analysis, revealed increased expression of LFA-1, CD25, CD44, 4F2, CD28 and the heat-stable antigen, whereas Thy-1 and CD5 decreased. Collectively, these results suggest an oligoclonal expansion and activation of gamma delta+ T cells in response to L. major infection.


Subject(s)
Leishmaniasis, Cutaneous/immunology , Receptors, Antigen, T-Cell, gamma-delta/immunology , T-Lymphocyte Subsets/immunology , Animals , Cell Cycle , Gene Rearrangement, delta-Chain T-Cell Antigen Receptor , Immunophenotyping , Leishmania major/immunology , Lymphocyte Activation , Lymphoid Tissue/cytology , Mice , Mice, Inbred C57BL , Mice, Inbred CBA
13.
Biochem J ; 295 ( Pt 2): 555-64, 1993 Oct 15.
Article in English | MEDLINE | ID: mdl-8240257

ABSTRACT

The major macromolecules on the surface of the parasitic protozoan Leishmania major appear to be down-regulated during transformation of the parasite from an insect-dwelling promastigote stage to an intracellular amastigote stage that invades mammalian macrophages. In contrast, the major parasite glycolipids, the glycoinositol phospholipids (GIPLs), are shown here to be expressed at near-constant levels in both developmental stages. The structures of the GIPLs from tissue-derived amastigotes have been determined by h.p.l.c. analysis of the deaminated and reduced glycan head groups, and by chemical and enzymic sequencing. The deduced structures appear to form a complete biosynthetic series, ranging from Man alpha 1-4GlcN-phosphatidylinositol (PI) to Gal alpha 1-3Galf beta 1-3Man alpha 1-3Man alpha 1-4GlcN-PI (GIPL-2). A small proportion of GIPL-2 was further extended by addition of a Gal residue in either alpha 1-6 or beta 1-3 linkage. From g.c.-m.s. analysis and mild base treatment, all the GIPLs were shown to contain either alkylacylglycerol or lyso-alkylglycerol lipid moieties, where the alkyl chains were predominantly C18:0, with lower levels of C20:0, C22:0 and C24:0. L. major amastigotes also contained at least two PI-specific phospholipase C-resistant glycolipids which are absent from promastigotes. These neutral glycolipids were resistant to both mild acid and mild base hydrolysis, contained terminal beta-Gal residues and were not lost during extensive purification of amastigotes from host cell membranes. It is likely that these glycolipids are glycosphingolipids acquired from the mammalian host. The GIPL profile of L. major amastigotes is compared with the profiles found in L. major promastigotes and L. donovani amastigotes.


Subject(s)
Glycolipids/metabolism , Leishmania major/metabolism , Phosphatidylinositols/metabolism , Animals , Carbohydrate Sequence , Chromatography, Thin Layer , Glycolipids/chemistry , Leishmania major/growth & development , Molecular Sequence Data , Phosphatidylinositol Diacylglycerol-Lyase , Phosphatidylinositols/chemistry , Phosphoric Diester Hydrolases/metabolism
14.
J Immunol ; 150(2): 550-5, 1993 Jan 15.
Article in English | MEDLINE | ID: mdl-8419488

ABSTRACT

To assess the importance of gamma delta + T lymphocyte responses in experimental murine cutaneous leishmaniasis, the expression of gamma delta + TCR on the surface of lymphocytes in spleen and draining lymph nodes of mice infected with Leishmania major was examined. In both susceptible BALB/c and resistant CBA/J mice, an increase in gamma delta + T cells was observed after s.c. infection. Whereas in chronically infected BALB/c mice, these cells can represent up to 35% of the CD3+ cells in the spleen, in CBA/J mice the percentage of gamma delta + T cells returns to a lower level after resolution of lesions. Further experiments, in which the disease outcome was modified in susceptible BALB/c and in resistant CBA/J mice by chemotherapy or immunointervention, have confirmed that a correlation existed between the multiplication of the parasites and the expansion of gamma delta + T cells in the spleen. Interestingly, in BALB/c mice infected for 4 mo, these gamma delta + T cells represent 80% of the activated blast population and a large proportion of them express the alpha-chain of the CD8 molecule. In an attempt to assess a potential role for the gamma delta + T cells in the control of infection with L. major, the effect of injection of mAb against gamma delta TCR on the course of infection was studied. In both BALB/c and CBA/J mice, blocking of the gamma delta TCR resulted in the development of larger lesions that contained increased numbers of parasites. This treatment significantly delayed the healing of cutaneous lesions in otherwise resistant CBA/J mice. Taken together these results indicate that gamma delta + T cells are expanded during experimental infection of mice with L. major and could be involved in host defense against this parasite.


Subject(s)
Leishmania tropica , Leishmaniasis, Cutaneous/immunology , Receptors, Antigen, T-Cell, gamma-delta/analysis , T-Lymphocytes/immunology , Animals , Antibodies, Monoclonal/immunology , CD4 Antigens/physiology , Leukocyte Count , Mice , Mice, Inbred BALB C , Mice, Inbred CBA
15.
Exp Parasitol ; 75(2): 196-206, 1992 Sep.
Article in English | MEDLINE | ID: mdl-1516667

ABSTRACT

During its life cycle, the protozoan parasite Leishmania major alternates from an intracellular amastigote form in the mammalian host to a flagellated promastigote form in the insect vector. The expression of the surface metalloprotease (PSP) during differentiation in vitro was investigated by Western and Northern blots, by immunoprecipitation of cells metabolically labeled with [35S]methionine or labeled at the surface with radioactive iodine, and by quantification of the proteolytic activity in substrate-containing polyacrylamide gels. We report that the surface metalloprotease is down-regulated at both the mRNA and the protein level in amastigotes, where it represents less than 1% of the equivalent proteolytic activity detected in promastigotes. A significant amount of mRNA is detected 4 hr after the onset of differentiation. The expression of the protease begins at that time and reaches steady state 8 hr later. The synthesis of PSP precedes the complete morphological differentiation to the promastigote stage and the appearance of the lipophosphoglycan, another major promastigote surface component. In contrast to PSP, a family of mercaptoethanol-activated proteases present in the amastigote exists only at a reduced level in the promastigote. The confinement of the surface metalloprotease to the insect stage of the parasite suggests that it has no physiological function in the parasitism maintenance of mammalian host macrophages.


Subject(s)
Leishmania tropica/enzymology , Metalloendopeptidases/biosynthesis , Animals , Down-Regulation , Endopeptidases/biosynthesis , Gene Expression Regulation, Enzymologic , Glycosphingolipids/biosynthesis , Leishmania tropica/cytology , Leishmania tropica/growth & development , Metalloendopeptidases/genetics , Metalloendopeptidases/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Protozoan/genetics , RNA, Protozoan/metabolism
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