Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Plant Biotechnol J ; 16(2): 649-659, 2018 02.
Article in English | MEDLINE | ID: mdl-28796437

ABSTRACT

The coleopteran insect western corn rootworm (WCR, Diabrotica virgifera virgifera) is an economically important pest in North America and Europe. Transgenic corn plants producing Bacillus thuringiensis (Bt) insecticidal proteins have been useful against this devastating pest, but evolution of resistance has reduced their efficacy. Here, we report the discovery of a novel insecticidal protein, PIP-47Aa, from an isolate of Pseudomonas mosselii. PIP-47Aa sequence shows no shared motifs, domains or signatures with other known proteins. Recombinant PIP-47Aa kills WCR, two other corn rootworm pests (Diabrotica barberi and Diabrotica undecimpunctata howardi) and two other beetle species (Diabrotica speciosa and Phyllotreta cruciferae), but it was not toxic to the spotted lady beetle (Coleomegilla maculata) or seven species of Lepidoptera and Hemiptera. Transgenic corn plants expressing PIP-47Aa show significant protection from root damage by WCR. PIP-47Aa kills a WCR strain resistant to mCry3A and does not share rootworm midgut binding sites with mCry3A or AfIP-1A/1B from Alcaligenes that acts like Cry34Ab1/Cry35Ab1. Our results indicate that PIP-47Aa is a novel insecticidal protein for controlling the corn rootworm pests.


Subject(s)
Bacillus thuringiensis/metabolism , Zea mays/metabolism , Zea mays/microbiology , Animals , Pest Control, Biological , Plants, Genetically Modified/metabolism , Plants, Genetically Modified/microbiology
2.
Science ; 354(6312): 634-637, 2016 Nov 04.
Article in English | MEDLINE | ID: mdl-27708055

ABSTRACT

The coleopteran insect western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte) is a devastating crop pest in North America and Europe. Although crop plants that produce Bacillus thuringiensis (Bt) proteins can limit insect infestation, some insect populations have evolved resistance to Bt proteins. Here we describe an insecticidal protein, designated IPD072Aa, that is isolated from Pseudomonas chlororaphis. Transgenic corn plants expressing IPD072Aa show protection from WCR insect injury under field conditions. IPD072Aa leaves several lepidopteran and hemipteran insect species unaffected but is effective in killing WCR larvae that are resistant to Bt proteins produced by currently available transgenic corn. IPD072Aa can be used to protect corn crops against WCRs.


Subject(s)
Bacterial Proteins/metabolism , Coleoptera/metabolism , Insecticide Resistance , Insecticides/metabolism , Plant Diseases/parasitology , Plant Roots/parasitology , Plants, Genetically Modified/parasitology , Pseudomonas chlororaphis/metabolism , Zea mays/parasitology , Animals , Bacillus thuringiensis Toxins , Bacterial Proteins/classification , Bacterial Proteins/genetics , Coleoptera/genetics , Crops, Agricultural/genetics , Crops, Agricultural/parasitology , Endotoxins/genetics , Endotoxins/metabolism , Hemolysin Proteins/genetics , Hemolysin Proteins/metabolism , Phylogeny , Plant Roots/genetics , Plants, Genetically Modified/genetics , Zea mays/genetics
SELECTION OF CITATIONS
SEARCH DETAIL
...