ABSTRACT
Molecular genetics appears to be the most promising approach to understanding the biology and pathology of Chlamydia. This report focuses on the cloning and the protein expression of a DNA fragment from Chlamydia trachomatis DK20 chromosome. Results of hybridization experiments suggest that this sequence is specifically present within chlamydial DNA. The coding capacity of this DNA fragment is supported by the expression of a 26,000 m.w. peptide, in an Escherichia coli maxicell system.
Subject(s)
Bacterial Proteins/genetics , Chlamydia trachomatis/genetics , Genes, Bacterial/genetics , Base Sequence , Cloning, Molecular , Escherichia coli/genetics , Genomic Library , Molecular Sequence DataABSTRACT
The Authors have tested the new Tb-Elisa method to detect specific antibodies, raised against A60 major mycobacterial antigen complex, in patients of groups II, III and IV of CDC classification suffering from acquired immunodeficiency and in HIV-1 negative control subjects. The test results support laboratory diagnosis of acute phase and reactivation of mycobacterial infection.
