ABSTRACT
Human strongyloidiasis research requires a large supply of Strongyloides stercoralis. This can be achieved through in vivo maintenance of Strongyloides stercoralis in Meriones unguiculatus, but isolating a large quantity of Strongyloides stercoralis to establish the colony from an infected patient is too difficult to achieve. Hence, Strongyloides ratti have been used as a model in human strongyloidiasis research. This study describes a successful establishment and maintenance of Strongyloides ratti infection in experimentally immunosuppressed Wistar rats. Large quantities of filariform (iL3) larvae of Strongyloides ratti for research related to human strongyloidiasis have been harvested following this protocol. Molecular detection method based on PCR using species specific primers was used to confirm the species of the harvested infective larvae (iL3). Additionally, the identification of histopathological lesions confirmed the presence of infective larvae (iL3) in the liver and lungs as a result of an increased parasite burden due to hyperinfection and disseminated disease. This pathological presentation was found to be similar to that reported in Strongyloides stercoralis-infected immunocompromised human subjects.
ABSTRACT
We investigated the immunogenicity of recombinant rMSP1 (rPbMSP1) that was generated from Plasmodium berghei. The rPbMSP1 formulated in alum was found to be immunogenic which induced high levels of specific anti-rPbMSP1 antibody. The IgG2a response predominated over IgG1 during the challenge infection in the vaccinated mice. Mice vaccinated with rPbMSP1 in alum mounted significant protective immunity against challenge infection (P < 0.01). On day 121 after the booster, three out of ten mice immunized with rPbMSP1 in PBS survived parasite infection (P < 0.05) and eight out of ten mice vaccinated with r MSP1 in alum did (P < 0.01). Hence, immunization with MSP1 in alum obviously has conferred protective effects, which prevented death from P. berghei lethal infection in mice (P < 0.01). These observations provide an excellent model for clinical assessment of this formulation in human subjects.
