ABSTRACT
BACKGROUND: Vulvar dermatofibrosarcoma is a rare fibrous tumor of intermediate grade malignancy, with a tendency for local recurrence, and rarely metastasizes. Management should be multidisciplinary. This is a report of an apparently benign vulvar mass with delayed diagnosis of vulvar dermatofibrosarcoma. CASE REPORT: A 42-year-old woman was referred to our hospital because of a vulvar tumor lasting 16 years, although several gynecological procedures and a total laparoscopic hysterectomy had been performed two years before. During this long period the lesion did not change morphological features and remained asymptomatic. Only a benign vulvar mass was diagnosed. Then, the swelling became evident showing erythematous skin with an aspect of "peau d'orange", leading the patient to consult a specialist. A firm vulvar swelling was observed in the anterior third of right labia majora continuing with about 3 cm of cord on top, quite movable above the underlying tissue but not on the overlying tissue. A wide excision was performed. The pathological examination showed positive margins. One month later an extensive deeper excision was performed. Histology confirmed a diagnosis of dermatofibrosarcoma. Immunohistochemistry was strongly positive for CD34. CONCLUSION: Vulvar lesions always require complete pathologic examination even in case of features of benign tumor to exclude a dermatofibrosarcoma. The role of the pathologist is essential to ensure negative microscopic margins and to avoid local recurrence.
Subject(s)
Dermatofibrosarcoma/pathology , Vulvar Neoplasms/pathology , Adult , Female , HumansABSTRACT
Mucous membrane pemphigoid (MMP) (also known as cicatricial pemphigoid) is a rare autoimmune mucocutaneous blistering disease that affects mucous membranes derived from stratified squamous epithelium and the skin. A subset of MMP affects only the oral cavity and is referred to as the oral pemphigoid (OP). MMP and OP are characterized by subepithelial vesicles on histology and in vivo deposition of immunoglobulins and complement at the basement membrane zone (BMZ) on immunopathology. Previous studies have shown that sera of patients with MMP bind to human integrin beta4, while sera of patients with oral pemphigoid bind to the integrin alpha6 component of the heterodimer. The prognosis in MMP is grave but excellent in OP. In this study we compare the binding of sera from patients with OP from Boston, MA, USA to Naples, Italy, and attempt to identify an epitope to which the anti-integrin alpha6 human autoantibody binds. Our results indicate that the sera from Boston and Naples are identical in their reactivity. They recognize a fragment I (AA 23-462) and its subfragment IB (AA 217-462) only, in the human integrin alpha6 molecule. Blocking studies, immunoprecipitation and immunoabsorbtion studies confirm the presence of this single 245 AA region. Antibodies to subfragment IB cause BMZ separation in organ culture using normal human oral mucosa as substrate. This preliminary study indicates that patients on both continents may have similar reactivity and suggests that an intercontinental study group could be established to advance our understanding of the pathogenesis of OP and the biology of anti-alpha6 integrin autoantibodies.
Subject(s)
Autoantibodies/immunology , Autoantigens/blood , Mouth Diseases/immunology , Mouth Mucosa/immunology , Pemphigoid, Bullous/immunology , Animals , Blotting, Western/methods , Case-Control Studies , Epitopes/analysis , Humans , Immunoglobulin Fragments/analysis , Immunoglobulin Fragments/immunology , Integrin alpha6/immunology , Italy , Organ Culture Techniques , Rabbits , United StatesABSTRACT
Brucellosis is endemic in the Mediterranean area. In spite of the false negative results, the standard agglutination test remains the routine test for the diagnosis of brucellosis in southern Italy. We present a case of a patient with undulant fever and erythema nodosum-like skin lesions, with negative serum agglutination test, but isolated positivity of the ELISA test for anti-Brucella IgM. A diagnosis of brucellosis for this patient was supported by the anamnestic and clinical data, and by the response to therapy. This case and a review of the literature urge us to consider the ELISA test indispensable for the serological diagnosis of brucellosis.
Subject(s)
Brucellosis/diagnosis , Enzyme-Linked Immunosorbent Assay , Erythema Nodosum/diagnosis , Immunoglobulin M/analysis , Adult , Animals , Brucellosis/immunology , Cattle , Erythema Nodosum/immunology , Female , Humans , Immunoglobulin M/immunology , Predictive Value of TestsABSTRACT
Kaposi's sarcoma (KS) of the tongue is extremely rare in immunocompetent patients. We report a case of KS of the tongue associated with a median rhomboid glossitis. The main clinical, pathological and immunohistochemical features allowed the differential diagnosis.
Subject(s)
Glossitis/pathology , Sarcoma, Kaposi/pathology , Tongue Neoplasms/pathology , Aged , Diagnosis, Differential , Glossitis/complications , Glossitis/diagnosis , Humans , Immunohistochemistry , Male , Sarcoma, Kaposi/complications , Sarcoma, Kaposi/diagnosis , Tongue Neoplasms/complications , Tongue Neoplasms/diagnosisABSTRACT
Canalicular adenoma is an uncommon benign neoplasm that occurs almost exclusively in the upper lip and, very rarely, in other sites. We describe a case arising in the left parotid gland as a firm, painful mass, in order to underline morphological and immunohistochemical findings, particularly in relation to differential diagnosis with low-grade carcinomas of the salivary glands.
Subject(s)
Adenoma/pathology , Parotid Neoplasms/pathology , Female , Humans , Immunohistochemistry , Middle AgedABSTRACT
Two cases of Merkel cell carcinoma (MCC) of the head and neck region are reported in order to stress their diversity in morphological, immunohistochemical and clinical findings. The remarkable variability of MCC has been analyzed, particularly in relation to the differential diagnosis and prognostic implications.
Subject(s)
Carcinoma, Merkel Cell/pathology , Head and Neck Neoplasms/pathology , Aged , Carcinoma, Merkel Cell/diagnosis , Carcinoma, Merkel Cell/metabolism , Diagnosis, Differential , Female , Head and Neck Neoplasms/diagnosis , Head and Neck Neoplasms/metabolism , Humans , Immunohistochemistry , MaleABSTRACT
The expression of cyclin T1 in an autoptic case of AIDS-related cachexia was investigated by immunohistochemistry. When contrasted with normal human tissues, a very similar pattern of expression was found. However, a peculiar distribution of cyclin T1 was noticed in the brown fat and in lymph nodes affected by AIDS-associated lymphadenopathy.
Subject(s)
Acquired Immunodeficiency Syndrome/metabolism , Cachexia/metabolism , Cyclins/biosynthesis , Acquired Immunodeficiency Syndrome/complications , Acquired Immunodeficiency Syndrome/pathology , Adipose Tissue, Brown/metabolism , Adipose Tissue, Brown/pathology , Adult , Cachexia/etiology , Cachexia/pathology , Cell Cycle , Cyclin T , Germinal Center/metabolism , Germinal Center/pathology , Humans , Immunoenzyme Techniques , Kidney/metabolism , Kidney/pathology , Lymph Nodes/metabolism , Lymph Nodes/pathology , Male , Organ Specificity , Thymus Gland/metabolism , Thymus Gland/pathologyABSTRACT
In this report we have investigated the effects of BAX in enhancing apoptosis in two primary non-small cell lung cancer cell lines. A count of the apoptotic cells by TUNEL staining revealed that almost 70% of BAX over-expressing cells died, while very few apoptotic cells were detectable in the wildtype cells or in the cells transfected with an empty vector. These findings suggest that de-regulated expression of BAX may provide a novel mechanism for initiating cell death in non-small cell lung cancer cells. Further studies are needed to better define the involvement of this protein in the complex mechanism of lung carcinogenesis and to definitely demonstrate the therapeutic utility of targeting this pathway.
Subject(s)
Apoptosis/genetics , Carcinoma, Non-Small-Cell Lung/genetics , Lung Neoplasms/genetics , Proto-Oncogene Proteins c-bcl-2 , Proto-Oncogene Proteins/genetics , Carcinoma, Non-Small-Cell Lung/metabolism , Carcinoma, Non-Small-Cell Lung/pathology , Genetic Therapy , Humans , Lung Neoplasms/metabolism , Lung Neoplasms/pathology , Proto-Oncogene Proteins/biosynthesis , Transfection , Tumor Cells, Cultured , bcl-2-Associated X ProteinABSTRACT
The authors describe a rare case of peritoneal mesothelioma. Cytological, histological and immunohistochemical characterization of the tumor allowed the differential diagnosis from papillary carcinoma and suggested the diagnosis of leiomyoid mesothelioma.
