Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 5 de 5
Filter
1.
S Afr Med J ; 106(9): 883-5, 2016 Aug 01.
Article in English | MEDLINE | ID: mdl-27601111

ABSTRACT

Human brucellosis in South Africa (SA) is under-diagnosed and under-reported. This is because many clinicians have little or no experience in managing affected patients, and in part because of the nonspecific and insidious nature of the disease. A case of human brucellosis caused by Brucella melitensis in a patient from the Western Cape Province of SA is described, and the resulting exposure of staff members at two medical microbiology laboratories, as well as the public health investigation that was conducted, are discussed. This article aims to highlight the need for strengthening integration between public health, medical and veterinary services and exposing deficiencies in public health, veterinary and laboratory practices.


Subject(s)
Brucella melitensis/isolation & purification , Brucellosis , Communicable Disease Control , Diagnostic Errors/prevention & control , Disease Notification , Adult , Animals , Brucellosis/diagnosis , Brucellosis/epidemiology , Brucellosis/prevention & control , Brucellosis/veterinary , Communicable Disease Control/methods , Communicable Disease Control/organization & administration , Communicable Disease Control/standards , Disease Notification/methods , Disease Notification/standards , Female , Humans , Interdisciplinary Communication , Intersectoral Collaboration , Male , South Africa/epidemiology , Veterinary Medicine/methods
2.
Bull World Health Organ ; 90(3): 191-199A, 2012 Mar 01.
Article in English | MEDLINE | ID: mdl-22461714

ABSTRACT

OBJECTIVE: To describe findings from an external quality assessment programme involving laboratories in Africa that routinely investigate epidemic-prone diseases. METHODS: Beginning in 2002, the Regional Office for Africa of the World Health Organization (WHO) invited national public health laboratories and related facilities in Africa to participate in the programme. Three surveys comprising specimens and questionnaires associated with bacterial enteric diseases, bacterial meningitis, plague, tuberculosis and malaria were sent annually to test participants' diagnostic proficiency. Identical surveys were sent to referee laboratories for quality control. Materials were prepared, packaged and shipped in accordance with standard protocols. Findings and reports were due within 30 days. Key methodological decisions and test results were categorized as acceptable or unacceptable on the basis of consensus feedback from referees, using established grading schemes. FINDINGS: Between 2002 and 2009, participation increased from 30 to 48 Member States of the WHO and from 39 to 78 laboratories. Each survey was returned by 64-93% of participants. Mean turnaround time was 25.9 days. For bacterial enteric diseases and meningitis components, bacterial identification was acceptable in 65% and 69% of challenges, respectively, but serotyping and antibiotic susceptibility testing and reporting were frequently unacceptable. Microscopy was acceptable for 73% of plague challenges. Tuberculosis microscopy was satisfactorily performed, with 87% of responses receiving acceptable scores. In the malaria component, 82% of responses received acceptable scores for species identification but only 51% of parasite quantitation scores were acceptable. CONCLUSION: The external quality assessment programme consistently identified certain functional deficiencies requiring strengthening that were present in African public health microbiology laboratories.


Subject(s)
Disease Outbreaks/prevention & control , Laboratories/standards , Population Surveillance/methods , Public Health/standards , Quality Assurance, Health Care/standards , Africa , Health Care Surveys , Health Services , Humans , Laboratories/statistics & numerical data , Malaria/diagnosis , Meningitis, Bacterial/diagnosis , Plague/diagnosis , Public Health/statistics & numerical data , Quality Assurance, Health Care/statistics & numerical data , Quality Control , Tuberculosis, Pulmonary/diagnosis
4.
PLoS One ; 6(5): e19688, 2011 May 11.
Article in English | MEDLINE | ID: mdl-21589919

ABSTRACT

Most of the species of fungi that cause disease in mammals, including Cryptococcus neoformans var. grubii (serotype A), are exogenous and non-contagious. Cryptococcus neoformans var. grubii is associated worldwide with avian and arboreal habitats. This airborne, opportunistic pathogen is profoundly neurotropic and the leading cause of fungal meningitis. Patients with HIV/AIDS have been ravaged by cryptococcosis--an estimated one million new cases occur each year, and mortality approaches 50%. Using phylogenetic and population genetic analyses, we present evidence that C. neoformans var. grubii may have evolved from a diverse population in southern Africa. Our ecological studies support the hypothesis that a few of these strains acquired a new environmental reservoir, the excreta of feral pigeons (Columba livia), and were globally dispersed by the migration of birds and humans. This investigation also discovered a novel arboreal reservoir for highly diverse strains of C. neoformans var. grubii that are restricted to southern Africa, the mopane tree (Colophospermum mopane). This finding may have significant public health implications because these primal strains have optimal potential for evolution and because mopane trees contribute to the local economy as a source of timber, folkloric remedies and the edible mopane worm.


Subject(s)
Cryptococcosis/microbiology , Cryptococcus neoformans/genetics , Evolution, Molecular , Cryptococcus neoformans/classification , Cryptococcus neoformans/pathogenicity , DNA, Fungal/genetics , Haplotypes , Humans , Molecular Sequence Data , Phylogeny , Recombination, Genetic
5.
J Clin Microbiol ; 49(1): 307-14, 2011 Jan.
Article in English | MEDLINE | ID: mdl-20980574

ABSTRACT

Compared to the incidence in adults, cryptococcosis is inexplicably rare among children, even in sub-Saharan Africa, which has the highest prevalence of coinfection with HIV and Cryptococcus neoformans. To explore any mycological basis for this age-related difference in the incidence of cryptococcosis, we investigated isolates of C. neoformans recovered from pediatric and adult patients during a 2-year period in South Africa. From reports to the Group for Enteric, Respiratory, and Meningeal Disease Surveillance in South Africa (GERMS-SA), we reviewed all cases of cryptococcosis in 2005 and 2006. We analyzed one isolate of C. neoformans from each of 82 pediatric patients (<15 years of age) and determined the multilocus sequence type (ST), mating type, ploidy, and allelic profile. This sample included isolates of all three molecular types of serotype A or C. neoformans var. grubii (molecular types VNI, VNII, and VNB) and one AD hybrid. Seventy-seven (94%) of the strains possessed the MATα mating type allele, and five were MATa. Seventy-five (91%) were haploid, and seven were diploid. A total of 24 different STs were identified. The ratios of each mating type and the proportion of haploids were comparable to those for the isolates that were obtained from 86 adult patients during the same period. Notably, the most prevalent pediatric ST was significantly associated with male patients. Overall, these pediatric isolates exhibited high genotypic diversity. They included a relatively large percentage of diploids and the rarely reported MATa mating type.


Subject(s)
Cryptococcosis/microbiology , Cryptococcus neoformans/classification , Cryptococcus neoformans/isolation & purification , Genetic Variation , Adolescent , Adult , Alleles , Child , Child, Preschool , Cryptococcus neoformans/genetics , DNA, Fungal/chemistry , DNA, Fungal/genetics , Female , Genes, Mating Type, Fungal , Genotype , Humans , Infant , Infant, Newborn , Male , Molecular Sequence Data , Multilocus Sequence Typing , Mycological Typing Techniques , Ploidies , Sequence Analysis, DNA , South Africa
SELECTION OF CITATIONS
SEARCH DETAIL
...