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1.
Exp Neurol ; 235(1): 273-81, 2012 May.
Article in English | MEDLINE | ID: mdl-22366325

ABSTRACT

Symptoms of Parkinson's disease typically emerge later in life when loss of nigrostriatal dopamine neuron function exceeds the threshold of compensatory mechanisms in the basal ganglia. Although nigrostriatal dopamine neurons are lost during aging, in Parkinson's disease other detrimental factors must play a role to produce greater than normal loss of these neurons. Early development has been hypothesized to be a potentially vulnerable period when environmental or genetic abnormalities may compromise central dopamine neurons. This study uses a specific parkinsonian neurotoxin, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), to probe the relative vulnerability of nigrostriatal dopamine neurons at different stages of primate development. Measures of dopamine, homovanillic acid, 1-methyl-pyridinium concentrations and tyrosine hydroxylase immunoreactive neurons indicated that at mid-gestation dopamine neurons are relatively vulnerable to MPTP, whereas later in development or in the young primate these neurons are resistant to the neurotoxin. These studies highlight a potentially greater risk to the fetus of exposure during mid-gestation to environmental agents that cause oxidative stress. In addition, the data suggest that uncoupling protein-2 may be a target for retarding the progressive loss of nigrostriatal dopamine neurons that occurs in Parkinson's disease and aging.


Subject(s)
Corpus Striatum/drug effects , Dopamine/metabolism , MPTP Poisoning/metabolism , Substantia Nigra/drug effects , Age Factors , Animals , Chlorocebus aethiops , Corpus Striatum/metabolism , Corpus Striatum/physiopathology , Homovanillic Acid/metabolism , MPTP Poisoning/physiopathology , Neurons/drug effects , Neurons/metabolism , Substantia Nigra/metabolism , Substantia Nigra/physiopathology , Tyrosine 3-Monooxygenase/metabolism
2.
Neuroscience ; 192: 500-6, 2011 Sep 29.
Article in English | MEDLINE | ID: mdl-21699961

ABSTRACT

Ghrelin, an orexigenic hormone produced by the stomach, increases food intake and enhances the locomotor and rewarding effects of cocaine. Consistent with these behavioral effects, ghrelin increases dopamine cell activity in the mesolimbic system resulting in elevated levels of dopamine release and turnover in target regions such as the ventral striatum. In the current study, we examined the psychostimulant effects of acute and daily cocaine in mice with targeted deletion of the ghrelin gene (ghrelin knockout; KO) and that of their wild-type (WT) littermates. We hypothesized that ghrelin-KO mice would be hyporesponsive to the effects of cocaine as reflected in attenuated locomotor activity following both acute and chronic injections, and that this would be correlated with striatal dopamine and dopamine metabolite concentrations. Results show that the locomotor stimulating effect of cocaine (10 mg/kg) was decreased in ghrelin-KO mice as compared with their WT littermates. In addition, repeated daily injection of cocaine resulted in gradual increases in locomotor activity in WT mice, an effect that was attenuated in ghrelin-KO mice. These behavioral effects were correlated with changes in dopamine utilization in the striatum of WT mice that were not seen in ghrelin-KO mice unless these were pretreated with ghrelin. These data suggest that ghrelin is important for normal function of the mesolimbic dopaminergic system, potentially modulating both dopamine release and reuptake.


Subject(s)
Cocaine/pharmacology , Dopamine Uptake Inhibitors/pharmacology , Ghrelin/metabolism , Motor Activity/drug effects , Animals , Dopamine/metabolism , Ghrelin/deficiency , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , Mice, Knockout
3.
Neuroscience ; 189: 277-85, 2011 Aug 25.
Article in English | MEDLINE | ID: mdl-21640165

ABSTRACT

Methamphetamine is a CNS stimulant with limited therapeutic indications, but is widely abused. Short-term exposure to higher doses, or long-term exposure to lower doses, of methamphetamine induces lasting damage to nigrostriatal dopamine neurons in man and animals. Strong evidence indicates that the mechanism for this detrimental effect on dopamine neurons involves oxidative stress exerted by reactive oxygen species. This study investigates the relative susceptibility of dopamine neurons in mid-gestation, young, and adult (not aged) monkeys to four treatments with methamphetamine over 2 days. Primate dopamine neurons undergo natural cell death at mid-gestation, and we hypothesized that during this event they are particularly vulnerable to oxidative stress. The results indicated that at mid-gestation and in adults, dopamine neurons were susceptible to methamphetamine-induced damage, as indicated by loss of striatal tyrosine hydroxylase (TH) immunoreactivity and dopamine concentration. However, dopamine neurons in young animals appeared totally resistant to the treatment, despite this group having higher brain levels of methamphetamine 3 h after administration than the adults. As a possible explanation for the protection, striatal glial-derived neurotrophic factor (GDNF) levels were elevated in young animals 1 week after treatment, but not in adults following methamphetamine treatment. Implications of these primate studies are: (1) the susceptibility of dopamine neurons at mid-gestation to methamphetamine warns against the risk of exposing pregnant women to the drug or oxidative stressors, and supports the hypothesis of Parkinson's disease being associated with oxidative stress during development, (2) elucidation of the mechanism of resistance of dopamine neurons in the young animals to methamphetamine-induced oxidative stress may provide targets for slowing or preventing age- or disease-related loss of adult nigrostriatal dopamine (DA) neurons, and (3) the increased striatal production of GDNF in young animals, but not in adults, in response to methamphetamine, suggests the possibility of an age-related change in the neurotrophic capacity of the striatal dopamine system.


Subject(s)
Aging/physiology , Central Nervous System Stimulants/pharmacology , Dopamine/metabolism , Methamphetamine/pharmacology , Neurons/drug effects , Parkinson Disease/etiology , Animals , Brain/metabolism , Central Nervous System Stimulants/pharmacokinetics , Chlorocebus aethiops , Glial Cell Line-Derived Neurotrophic Factor/metabolism , Methamphetamine/pharmacokinetics , Neurons/metabolism , Tissue Distribution , Tyrosine 3-Monooxygenase/metabolism
4.
Pharmacol Biochem Behav ; 99(3): 324-32, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21620883

ABSTRACT

Parkinson's Disease (PD) and the natural aging process share a number of biochemical mechanisms, including reduced function of dopaminergic systems. The present study aims to determine the extent that motor and behavioral changes in aged monkeys resemble parkinsonism induced by the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. The behavioral and physiological changes in PD are believed to result largely from selective depletion of dopamine in the nigrostriatal system. In the present study, ten aged female monkeys were compared with three groups: 9 untreated young adult female monkeys, 10 young adult male monkeys and 13 older male monkeys that had been exposed to MPTP. Trained observers, blind as to age and drug condition and without knowledge of the hypotheses, scored the monkeys using the Parkinson's factor score (Parkscore), which has been validated by a high correlation with post mortem striatal dopamine (DA) concentrations. The aged animals had higher scores on the Parkscore compared with the young adults, with most of its component behavioral items showing significance (tremor, Eating Problems, Delayed initiation of movement, and Poverty of Movement). L-Dopa and DA-agonists did not clearly reverse the principal measure of parkinsonism. DA concentrations post mortem were 63% lower in 3 aged monkeys in the ventral putamen compared with 4 young adults, with greater reductions in putamen than in caudate (45%). We conclude that aged monkeys, unexposed to MPTP, show a similar profile of parkinsonism to that seen after the neurotoxin exposure to MPTP in young adult monkeys. The pattern of greater DA depletion in putamen than in caudate in aged monkeys is the same as in human Parkinson's disease and contrasts with the greater depletion in caudate seen after MPTP. Aged monkeys of this species reflect many facets of Parkinson's disease, but like older humans do not improve with standard dopamine replacement pharmacotherapies.


Subject(s)
Aging/metabolism , Caudate Nucleus/metabolism , Disease Models, Animal , Dopamine/metabolism , Parkinsonian Disorders/metabolism , Putamen/metabolism , Aging/drug effects , Aging/pathology , Animals , Caudate Nucleus/drug effects , Chlorocebus aethiops , Dopamine Agonists/pharmacology , Dopamine Agonists/therapeutic use , Female , Haplorhini , Male , Parkinson Disease/drug therapy , Parkinson Disease/metabolism , Parkinson Disease/pathology , Parkinsonian Disorders/drug therapy , Parkinsonian Disorders/pathology , Putamen/drug effects
5.
J Comp Neurol ; 515(1): 31-40, 2009 Jul 01.
Article in English | MEDLINE | ID: mdl-19399891

ABSTRACT

In spite of partial success in treating Parkinson's disease by using ectopically placed grafts of dopamine-producing cells, restoration of the original neuroanatomical circuits, if possible, might work better. Previous evidence of normal anatomic projections from ventral mesencephalic (VM) grafts placed in the substantia nigra (SN) has been limited to neonatal rodents and double grafting or bridging procedures. This study attempted to determine whether injection of a potent growth-promoting factor, glial cell line-derived neurotrophic factor (GDNF), into the target regions or placement of fetal striatal co-grafts in the nigrostriatal pathway might elicit neuritic outgrowth to the caudate nucleus. Four adult St. Kitts green monkeys received embryonic VM grafts into the rostral mesencephalon near the host SN, and injections of adeno-associated virus 2 (AAV2)/GDNF or equine infectious anemia virus (EIAV)/GDNF into the caudate. Three adult monkeys were co-grafted with fetal VM tissue near the SN and fetal striatal grafts (STR) 2.5 mm rostral in the nigrostriatal pathway. Before sacrifice, the striatal target regions were injected with the retrograde tracer Fluoro-Gold (FG). FG label was found in tyrosine hydroxylase-labeled neurons in VM grafts in the SN of only those monkeys that received AAV2/GDNF vector injections into the ipsilateral striatum. All monkeys showed FG labeling in the host SN when FG labeling was injected on the same side. These data show that grafted dopaminergic neurons can extend neurites to a distant target releasing an elevated concentration of GDNF, and suggest that grafted neurons can be placed into appropriate loci for potential tract reconstruction.


Subject(s)
Brain Tissue Transplantation/methods , Corpus Striatum/metabolism , Embryonic Stem Cells/transplantation , Glial Cell Line-Derived Neurotrophic Factor/metabolism , Stem Cell Transplantation/methods , Substantia Nigra/transplantation , Animals , Cell Differentiation/physiology , Cells, Cultured , Chlorocebus aethiops , Corpus Striatum/cytology , Corpus Striatum/physiopathology , Disease Models, Animal , Graft Survival/physiology , Growth Cones/metabolism , Growth Cones/ultrastructure , Male , Neural Pathways/cytology , Neural Pathways/metabolism , Neurites/metabolism , Neurites/ultrastructure , Parkinsonian Disorders/metabolism , Parkinsonian Disorders/physiopathology , Parkinsonian Disorders/surgery , Staining and Labeling , Stilbamidines , Substantia Nigra/cytology , Substantia Nigra/metabolism , Treatment Outcome , Up-Regulation/physiology
6.
Cell Transplant ; 17(4): 427-44, 2008.
Article in English | MEDLINE | ID: mdl-18522245

ABSTRACT

Transplantation of embryonic dopamine (DA) neurons has been tested as a therapy for Parkinson's disease. Most studies placed DA neurons into the striatum instead of the substantia nigra (SN). Reconstruction of this DA pathway could serve to establish a more favorable environment for control of DA release by grafted neurons. To test this we used cografts of striatum to stimulate growth of DA axons from embryonic SN that was implanted adjacent to the host SN in African green monkeys. Embryonic striatum was implanted at one of three progressive distances rostral to the SN. Immunohistochemical analysis revealed DA neuron survival and neuritic outgrowth from the SN grafts at 12-36 weeks after grafting. Each animal showed survival of substantial numbers of DA neurons. Most fibers that exited SN grafts coursed rostrally. Striatal grafts showed evidence of target-directed outgrowth and contained dense patterns of DA axons that could be traced from their origin in the SN grafts. A polarity existed for DA neurites that exited the grafts; that is, those seen caudal to the grafts did not appear to be organized into a directional outflow while those on the rostral side were arranged in linear profiles coursing toward the striatal grafts. Some TH fibers that reached the striatal grafts appeared to arise from the residual DA neurons of the SN. These findings suggest that grafted DA neurons can extend neurites toward a desired target over several millimeters through the brain stem and caudal diencephalon of the monkey brain, which favors the prospect of circuit reconstruction from grafted neurons placed into appropriate locations in their neural circuitry. Further study will assess the degree to which this approach can be used to restore motor balance in the nonhuman primate following neural transplantation.


Subject(s)
Brain Tissue Transplantation , Corpus Striatum/transplantation , Fetal Tissue Transplantation , Substantia Nigra/transplantation , Animals , Biomarkers/metabolism , Cercopithecidae , Corpus Striatum/cytology , Dopamine/metabolism , Humans , Male , Neurons/cytology , Neurons/metabolism , Substantia Nigra/cytology , Substantia Nigra/embryology
7.
Exp Neurol ; 211(1): 252-8, 2008 May.
Article in English | MEDLINE | ID: mdl-18346734

ABSTRACT

Neural transplantation offers the potential of treating Parkinson's disease by grafting fetal dopamine neurons to depleted regions of the brain. However, clinical studies of neural grafting in Parkinson's disease have produced only modest improvements. One of the main reasons for this is the low survival rate of transplanted neurons. The inadequate supply of critical neurotrophic factors in the adult brain is likely to be a major cause of early cell death and restricted outgrowth of fetal grafts placed into the mature striatum. Glial derived neurotrophic factor (GDNF) is a potent neurotrophic factor that is crucial to the survival, outgrowth and maintenance of dopamine neurons, and so is a candidate for protecting grafted fetal dopamine neurons in the adult brain. We found that implantation of adeno-associated virus type 2 encoding GDNF (AAV2-GDNF) in the normal monkey caudate nucleus induced overexpression of GDNF that persisted for at least 6 months after injection. In a 6-month within-animal controlled study, AAV2-GDNF enhanced the survival of fetal dopamine neurons by 4-fold, and increased the outgrowth of grafted fetal dopamine neurons by almost 3-fold in the caudate nucleus of MPTP-treated monkeys, compared with control grafts in the other caudate nucleus. Thus, the addition of GDNF gene therapy to neural transplantation may be a useful strategy to improve treatment for Parkinson's disease.


Subject(s)
Corpus Striatum/metabolism , Dopamine/metabolism , Fetal Tissue Transplantation/methods , Glial Cell Line-Derived Neurotrophic Factor/physiology , MPTP Poisoning/pathology , MPTP Poisoning/surgery , Animals , Chlorocebus aethiops , Dependovirus/physiology , Disease Models, Animal , Embryo, Mammalian , Gene Transfer Techniques , Glial Cell Line-Derived Neurotrophic Factor/biosynthesis , Male , Time Factors , Tyrosine 3-Monooxygenase/metabolism
8.
Neuroscience ; 147(2): 419-27, 2007 Jun 29.
Article in English | MEDLINE | ID: mdl-17543464

ABSTRACT

Children exposed to cocaine during gestation have a higher incidence of neurobehavioral deficits. The neurochemical bases of these deficits have not been determined, but the pharmacology of cocaine and the nature of the abnormalities suggest that disruptions in catecholaminergic systems may be involved. In the current study, we used a rat model of prenatal cocaine exposure to examine the impact that this exposure has on the locus coeruleus (LC) noradrenergic system in offspring. Pregnant rats received twice-daily i.v. injections of cocaine (3 mg/kg) or saline between gestational days 10 and 20, and progeny were tested as juveniles. Exposure to a mild stressor elevated an index of norepinephrine turnover in the prefrontal cortex and also increased Fos expression in tyrosine hydroxylase-positive LC neurons in rats exposed to prenatal cocaine but not in rats exposed to prenatal saline. No change in the number of tyrosine hydroxylase-positive neurons in the LC was observed between the two prenatal treatment groups. Specific binding of [125I]-para-iodoclonidine, a radioligand with specificity for high affinity alpha2A-adrenergic receptors, was decreased in the LC of rats exposed to prenatal cocaine compared with prenatal saline controls. As alpha2-adrenergic receptors on LC norepinephrine neurons function as autoreceptors, their down-regulation by prenatal cocaine exposure provides a plausible mechanism for the observed heightened reactivity of norepinephrine neurons in these animals. These data indicate that prenatal cocaine exposure results in lasting changes to the regulation and responsivity of rat LC norepinephrine neurons. A similar dysregulation of LC norepinephrine neurons may occur in children exposed to cocaine during gestation, and this may explain, at least partly, the increased incidence of cognitive deficits that have been observed in these subjects.


Subject(s)
Autoreceptors/physiology , Cocaine/toxicity , Locus Coeruleus/physiology , Neurons/physiology , Norepinephrine/physiology , Prenatal Exposure Delayed Effects/physiopathology , Animals , Autoradiography , Clonidine/metabolism , Cocaine/administration & dosage , Female , Genes, fos/genetics , Immunohistochemistry , Injections, Intravenous , Locus Coeruleus/cytology , Male , Neurons/metabolism , Norepinephrine/metabolism , Prefrontal Cortex/drug effects , Prefrontal Cortex/physiology , Pregnancy , Radioligand Assay , Rats , Rats, Sprague-Dawley , Stress, Psychological/physiopathology , Tyrosine 3-Monooxygenase/metabolism
9.
Cell Transplant ; 14(4): 183-92, 2005.
Article in English | MEDLINE | ID: mdl-15929553

ABSTRACT

Neural stem cells (NSC) have been shown to migrate towards damaged areas, produce trophic factors, and replace lost cells in ways that might be therapeutic for Parkinson's disease (PD). However, there is very little information on the effects of NSC on endogenous cell populations. In the current study, effects of implanted human NSC (hNSC) on endogenous tyrosine hydroxylase-positive cells (TH+ cells) after treatment with 1-methyl-4-phenyl-1,2,3,6-tetra-hydropyridine (MPTP) were explored in nonhuman primates. After MPTP damage and in PD, the primate brain is characterized by decreased numbers of dopamine neurons in the substantia nigra (SN) and an increase in neurons expressing TH in the caudate nucleus. To determine how implanted NSC might affect these cell populations, 11 St. Kitts African green monkeys were treated with the selective dopaminergic neurotoxin, MPTP. Human NSC were implanted into the left and right caudate nucleus and the right SN of eight of the MPTP-treated monkeys. At either 4 or 7 months after NSC implants, the brains were removed and the size and number of TH+ cells in the target areas were assessed. The results were compared to data obtained from normal untreated control monkeys and to the three unimplanted MPTP-treated monkeys. The majority of hNSC were found bilaterally along the nigrostriatal pathway and in the substantia nigra, while relatively few were found in the caudate. In the presence of NSC, the number and size of caudate TH+ cells returned to non-MPTP-treated control levels. MPTP-induced and hNSC-induced changes in the putamen were less apparent. We conclude that after MPTP treatment in the primate, hNSC prevent the MPTP-induced upregulation of TH+ cells in the caudate and putamen, indicating that hNSC may be beneficial to maintaining a normal striatal environment.


Subject(s)
Brain Tissue Transplantation , MPTP Poisoning/therapy , Neostriatum/cytology , Neurons/cytology , Stem Cell Transplantation , Animals , Cell Size , Chlorocebus aethiops , Humans , Male , Neurons/enzymology , Transplantation, Heterologous , Tyrosine 3-Monooxygenase/metabolism
10.
Synapse ; 42(2): 80-3, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11574943

ABSTRACT

Children whose mothers used cocaine during pregnancy appear to have an increased incidence of certain neurobehavioral deficits. Rodent models of prenatal cocaine exposure have mimicked these deficits in the offspring, yet the biochemical basis of the behavioral abnormalities is unknown. We have been able to reproduce short-term memory deficits in our rat intravenous model of prenatal cocaine exposure, and as short-term memory is dependent on the function of dopamine neurons innervating the medial prefrontal cortex, we hypothesized that prenatal cocaine induces a dysfunction in the regulation of this pathway. Here we report that mild footshock stress, which preferentially activates the mesoprefrontal dopamine system, leads to an enhanced increase in dopamine turnover in the ventromedial prefrontal cortex of adolescent (postnatal day 35-37) rats exposed to cocaine in utero, suggesting that the dopamine neurons innervating this region are hyperresponsive in these rats. Thus, this biochemical alteration may be central to some of the cognitive deficits exhibited by offspring that were exposed to cocaine during fetal development.


Subject(s)
Cocaine/pharmacology , Dopamine/metabolism , Prefrontal Cortex/drug effects , Prenatal Exposure Delayed Effects , Stress, Physiological/metabolism , Ventral Tegmental Area/drug effects , 3,4-Dihydroxyphenylacetic Acid/metabolism , Aging/drug effects , Aging/physiology , Animals , Cocaine-Related Disorders/metabolism , Cocaine-Related Disorders/physiopathology , Electric Stimulation/adverse effects , Female , Gyrus Cinguli/drug effects , Gyrus Cinguli/embryology , Gyrus Cinguli/metabolism , Neural Pathways/drug effects , Neural Pathways/embryology , Neural Pathways/metabolism , Neurons/drug effects , Neurons/metabolism , Prefrontal Cortex/embryology , Prefrontal Cortex/metabolism , Pregnancy , Rats , Stress, Physiological/physiopathology , Ventral Tegmental Area/embryology , Ventral Tegmental Area/metabolism
11.
Synapse ; 41(4): 337-44, 2001 Sep 15.
Article in English | MEDLINE | ID: mdl-11494404

ABSTRACT

Prenatal exposure to cocaine has been shown to result in poor cognitive performance in the resulting offspring in humans and laboratory animals. The underlying biochemical changes that contribute to these behavioral effects are not known but have been proposed to involve changes in dopaminergic function. In these studies, we exposed rats to cocaine in utero using the clinically relevant intravenous model and report a mean loss of 24.8% of the tyrosine hydroxylase immunoreactive, presumed dopaminergic, neurons in the A10, but not A9 and A8, cell groups of the young adult offspring. Additionally, in prenatal cocaine-exposed rats dopaminergic neurons in the ventral, midline A10, and lateral A9 regions demonstrated a hyperreactivity to environmental stress, as measured by activation of the immediate-early gene, Fos. Mild, intermittent footshock did not further increase the number of dopamine neurons expressing Fos in prenatal cocaine-exposed rats, as it did in the prenatal saline controls. Because the exposure to cocaine took place during development, other potential changes in dopaminergic and nondopaminergic neuronal systems could result from the cocaine-induced reduction in numbers of A10 dopamine neurons. We hypothesize that a perinatal loss of A10 dopamine neurons, and subsequent developmental changes, contributes to a dysregulation of the adult mesoprefrontal system, resulting in the reported cognitive deficits.


Subject(s)
Cocaine/pharmacology , Dopamine Uptake Inhibitors/pharmacology , Dopamine/physiology , Neurons/drug effects , Prenatal Exposure Delayed Effects , Stress, Physiological/physiopathology , Animals , Cell Count , Cognition Disorders/chemically induced , Cognition Disorders/physiopathology , Electroshock , Environmental Exposure , Female , Immunohistochemistry , Male , Mesencephalon/cytology , Neurons/chemistry , Neurons/metabolism , Pregnancy , Proto-Oncogene Proteins c-fos/analysis , Proto-Oncogene Proteins c-fos/biosynthesis , Rats , Tyrosine 3-Monooxygenase/analysis
12.
Neuropsychopharmacology ; 24(5): 576-89, 2001 May.
Article in English | MEDLINE | ID: mdl-11282258

ABSTRACT

The reinforcing properties of nicotine and psychomotor stimulants are thought to be mediated through the mesolimbic dopamine (DA) system. This study investigates the role of high affinity nicotinic acetylcholine receptors (nAChRs) in cocaine place preference and examines some neurochemical changes in the mesolimbic DA system that might account for the interaction between nicotine and cocaine. 5 mg/kg is the lowest dose of cocaine able to condition a place preference in C57Bl/6 mice. Co-treatment with the nicotinic antagonist mecamylamine (1.0 mg/kg) disrupted place preference to 5 mg/kg cocaine. In addition, mice lacking the high affinity nAChR containing the beta2 subunit showed decreased place preference to 5 mg/kg cocaine, although higher doses of cocaine could condition a place preference in these knock out animals. In contrast, co-administration of a low dose of nicotine (0.2 mg/kg) potentiated place preference to a subthreshold dose of cocaine (3 mg/kg). DA turnover was monitored in several brain regions using tissue levels of DA and its primary metabolite DOPAC as an indication of DA release. Wild type mice showed decreased DA turnover following treatment with 5 mg/kg cocaine; whereas, this response was not seen in mice lacking the beta2 subunit of the nAChR. Induction of chronic fos-related antigens by cocaine was also reduced in mutant mice as compared to their wild type siblings, implying that downstream actions of cocaine were also affected by inactivation of the high affinity nAChR. These data indicate that activation of the high affinity nAChR may contribute to cocaine reinforcement.


Subject(s)
Cocaine-Related Disorders/metabolism , Cocaine/pharmacology , Dopamine/metabolism , Neurons/drug effects , Nucleus Accumbens/drug effects , Receptors, Nicotinic/drug effects , Receptors, Nicotinic/deficiency , 3,4-Dihydroxyphenylacetic Acid/metabolism , Acetylcholine/metabolism , Animals , Cholinergic Fibers/drug effects , Cholinergic Fibers/metabolism , Cocaine-Related Disorders/physiopathology , Conditioning, Psychological/drug effects , Conditioning, Psychological/physiology , Dose-Response Relationship, Drug , Female , Male , Mice , Mice, Knockout , Neurons/metabolism , Nicotine/pharmacology , Nucleus Accumbens/metabolism , Receptors, Nicotinic/genetics
13.
Biol Psychiatry ; 49(3): 300-6, 2001 Feb 01.
Article in English | MEDLINE | ID: mdl-11230881

ABSTRACT

BACKGROUND: We have examined the effects of nicotine pretreatment on dopaminergic and behavioral responses to conditioned fear stress in the rat. METHODS: Rats were pretreated daily with saline or nicotine for 20 days then challenged with nicotine or saline on day 21. Animals were trained in a classical conditioned fear paradigm. Dopamine utilization in the medial prefrontal cortex and nucleus accumbens shell and conditioned fear stress-induced immobility responses were assessed. RESULTS: Saline pretreated animals rapidly acquired the conditioned fear stress response as assessed by preferential activation of mesoprefrontal dopamine metabolism and tone-elicited immobility responses. Repeated, but not acute, nicotine pretreatment significantly reduced conditioned fear stress-induced dopamine metabolism in the medial prefrontal cortex and nucleus accumbens shell. Repeated nicotine pretreatment did not modify the acquisition or expression of conditioned fear stress responses, however. CONCLUSIONS: The dissimilar effects of repeated nicotine exposure on the cortical dopamine and behavioral responses to conditioned fear stress suggest that nicotine differs from other agents with anxiolytic activity that produce coordinated changes in conditioned fear stress-induced cortical dopaminergic and behavioral responses. Furthermore, compared with results of acute footshock stress, repeated nicotine pretreatment appears to have differential effects on physical versus psychological stressors. Results are discussed within the clinical context of stress-related psychopathology syndromes and comorbid nicotine dependence.


Subject(s)
Conditioning, Classical/drug effects , Fear/drug effects , Nicotine/pharmacology , Premedication , Receptors, Dopamine/drug effects , Animals , Arousal/drug effects , Dopamine/metabolism , Male , Motor Activity/drug effects , Nucleus Accumbens/drug effects , Prefrontal Cortex/drug effects , Rats , Rats, Sprague-Dawley , Stress, Psychological/complications
14.
J Neurosci ; 20(23): 8604-9, 2000 Dec 01.
Article in English | MEDLINE | ID: mdl-11102464

ABSTRACT

There are sexual differences in several parameters of the nigrostriatal dopamine neurons, as well as in the progression of diseases associated with this system, e.g., Parkinson's disease and dementia. These differences, as well as direct experimental data in rodents, suggest that gonadal hormones play a role in modulating this system. To determine whether circulating estrogen might have long-term effects by altering the number of dopamine neurons, the density of dopamine neurons was calculated in the compact zone of the substantia nigra of male and intact female short- (10 d) and longer-term (30 d) ovariectomized and short- and longer-term ovariectomized but estrogen-replaced nonhuman primates (African green monkeys). Furthermore, the number of tyrosine hydroxylase-expressing neurons, the total number of all types of neurons, and the volume of the compact zone of the substantia nigra were calculated in 30 d ovariectomized and in 30 d ovariectomized and estrogen-replaced monkeys. Unbiased stereological analyses demonstrated that a 30 d estrogen deprivation results in an apparently permanent loss of >30% of the total number of substantia nigra dopamine cells. Furthermore, the density calculations showed that brief estrogen replacement restores the density of tyrosine hydroxylase-immunoreactive cells after a 10 d, but not after a 30 d, ovariectomy. Moreover, the density of dopamine cells is higher in females than in males. These observations show the essential role of estrogen in maintaining the integrity of the nigral dopamine system, suggest a new treatment strategy for patients with Parkinson's disease and with certain forms of memory-impairing disorders, and provide another rationale for estrogen replacement therapy for postmenopausal women.


Subject(s)
Estrogens/administration & dosage , Memory , Neurons/drug effects , Parkinson Disease/metabolism , Substantia Nigra/drug effects , Animals , Cell Count , Cell Survival/drug effects , Chlorocebus aethiops , Dopamine/metabolism , Drug Implants , Estrogens/blood , Female , Male , Memory/physiology , Neurons/cytology , Neurons/metabolism , Ovariectomy , Parkinson Disease/etiology , Substantia Nigra/cytology , Substantia Nigra/metabolism , Tyrosine 3-Monooxygenase/metabolism
15.
Prog Brain Res ; 126: 433-53, 2000.
Article in English | MEDLINE | ID: mdl-11105661

ABSTRACT

We have discussed the role of dopamine in modulating the interactions between cortical and striatal regions that are involved in behavioral regulation. The evidence reviewed seems to suggest that dopamine acts, overall, to promote stimulus-induced responding for conditioned or reward-related stimuli by integrative actions at multiple forebrain sites. It is thus not surprising that dopaminergic dysfunction has been implicated in a number of neuropsychiatric disorders that involve abnormal cognitive and affective function. Future studies aimed at pinpointing the precise anatomical sites of action and molecular mechanisms involved in dopaminergic transmission within the corticolimbic circuit are critical for trying to disentangle the cellular mechanisms by which dopamine exerts its actions. Moreover, the afferent control of dopamine neurons from brainstem and forebrain sites need to be fully explored in order to begin to understand what mechanisms are involved in regulating the dopaminergic response to stimuli with incentive value. Finally, the post-synaptic consequences of prolonged and supranormal dopaminergic activation need to be investigated in order to understand what persistent neuroadaptations result from chronic activation of this neuromodulatory system (e.g. in drug addiction). Answers to these sorts of questions will undoubtedly provide important insights into the nature of dopaminergic function in the animal and human brain.


Subject(s)
Behavior/physiology , Corpus Striatum/physiology , Dopamine/physiology , Mental Disorders/physiopathology , Prefrontal Cortex/physiology , Psychotropic Drugs/pharmacology , Amphetamines/pharmacology , Amygdala/drug effects , Amygdala/physiology , Animals , Behavior/drug effects , Brain Mapping , Central Nervous System Stimulants/pharmacology , Cognition/drug effects , Cognition/physiology , Corpus Striatum/drug effects , Corpus Striatum/physiopathology , Depression/physiopathology , Dopamine Agonists/pharmacology , Emotions/drug effects , Emotions/physiology , Haplorhini , Humans , Learning/drug effects , Learning/physiology , Limbic System/drug effects , Limbic System/physiology , Limbic System/physiopathology , Mammals/physiology , Mammals/psychology , Memory/drug effects , Memory/physiology , Mental Disorders/drug therapy , Neural Pathways/drug effects , Neural Pathways/physiology , Neurotransmitter Agents/physiology , Nucleus Accumbens/drug effects , Nucleus Accumbens/physiology , Phencyclidine/pharmacology , Phencyclidine/toxicity , Prefrontal Cortex/drug effects , Prefrontal Cortex/physiopathology , Psychotropic Drugs/therapeutic use , Receptors, Neurotransmitter/drug effects , Receptors, Neurotransmitter/physiology , Reward , Schizophrenia/physiopathology , Substance-Related Disorders/physiopathology
16.
J Pharmacol Exp Ther ; 295(1): 58-66, 2000 Oct.
Article in English | MEDLINE | ID: mdl-10991961

ABSTRACT

Schizophrenics have cortical dysfunction that may involve mesoprefrontal dopamine (DA) systems. Rates of nicotine dependence approach 90% in schizophrenia, and nicotine administration through cigarette smoking may ameliorate cognitive dysfunction, which may be related to cortical DA dysregulation. We have shown that repeated, but not acute, nicotine pretreatment (0.15 mg/kg daily s.c.) reduces footshock stress-induced mesoprefrontal DA metabolism and immobility responses. This effect of repeated nicotine is dependent on mecamylamine (MEC)-sensitive nicotinic acetylcholine receptor (nAChR) stimulation and endogenous opioid peptides. In the present study, we have further characterized these effects of repeated nicotine on the stress reactivity of mesoprefrontal DA neurons by using the following: 1) local infusion of MEC into cell bodies (ventral tegmental area) and terminal fields (medial prefrontal cortex) to determine the site of action of nicotine; and 2) systemic administration of selective nAChR antagonists. Results of bilateral local infusions of MEC (0.1-1.0 microgram/side) into ventral tegmental area or medial prefrontal cortex in saline- and nicotine-pretreated rats suggests a modulatory role for somatodendritic versus terminal field nAChRs on mesoprefrontal DA neurons under stress-induced states. Experiments with dihydro-beta-erythroidine (a beta2-subunit-selective blocker; 0.0-3.0 mg/kg) and methylycaconitine (an alpha7-subunit-selective blocker; 0.0-8.4 mg/kg) suggest that both alpha4beta2- and alpha7-containing nAChRs modulate mesoprefrontal DA neurons. Thus, complex regulation of mesoprefrontal DA neurons by nAChRs is suggested, which may have relevance to prefrontal cortical DA dysfunction and the high comorbid rates of nicotine dependence in schizophrenia.


Subject(s)
Dopamine/metabolism , Nicotinic Antagonists/pharmacology , Prefrontal Cortex/drug effects , Animals , Dihydro-beta-Erythroidine/pharmacology , Dose-Response Relationship, Drug , Male , Mecamylamine/pharmacology , Prefrontal Cortex/metabolism , Rats , Rats, Sprague-Dawley , Ventral Tegmental Area/drug effects
17.
Brain Res Bull ; 52(6): 519-23, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10974491

ABSTRACT

Working memory has been proposed to require the proper functioning of the medial prefrontal cortex and its dopaminergic innervation. The dopaminergic input to the medial prefrontal cortex has been demonstrated to be sensitive to physical and psychological stress. In this report, we demonstrate that a brief exposure to 2, 5-dihydro-2,4,5-trimethylthiazoline (TMT), an odor derived from a predator of the rat, the fox, resulted in elevated dopamine metabolism in the medial prefrontal cortex and elevated serum corticosterone. We tested the effects of this olfactory stress on working memory using a spontaneous, delayed, non-matching-to-sample task using object recognition methods. Rats were exposed to one set of objects and, after a delay of 1, 15 or 60 min, later demonstrated a robust working memory of the familiar object compared to a novel object. When rats were exposed to TMT during the 15-min delay, working memory was disrupted without altering exploratory behavior. We conclude from these studies that (1) TMT selectively activates mesoprefrontal dopamine neurons, (2) TMT exposure can disrupt working memory and (3) this disruption in working memory is not due to an overall suppression of exploratory behavior but may involve altered mesoprefrontal dopaminergic activity.


Subject(s)
Dopamine/metabolism , Memory, Short-Term/drug effects , Neural Pathways/drug effects , Prefrontal Cortex/drug effects , Stress, Psychological/physiopathology , Thiazoles/pharmacology , Ventral Tegmental Area/drug effects , Animals , Corticosterone/blood , Corticosterone/metabolism , Fear/drug effects , Fear/physiology , Male , Memory, Short-Term/physiology , Neural Pathways/cytology , Neural Pathways/metabolism , Odorants , Olfactory Pathways/cytology , Olfactory Pathways/drug effects , Olfactory Pathways/metabolism , Prefrontal Cortex/cytology , Prefrontal Cortex/metabolism , Psychomotor Performance/drug effects , Psychomotor Performance/physiology , Rats , Rats, Sprague-Dawley , Reaction Time/drug effects , Reaction Time/physiology , Stress, Psychological/chemically induced , Ventral Tegmental Area/cytology , Ventral Tegmental Area/metabolism
18.
Biol Psychiatry ; 48(5): 415-24, 2000 Sep 01.
Article in English | MEDLINE | ID: mdl-10978725

ABSTRACT

BACKGROUND: Impulsivity associated with frontal cortical dysfunction appears to be a direct consequence of chronic consumption of drugs of abuse, though few investigations in animals have attempted to directly address this issue. In this study the effects of repeated, intermittent administration of a psychotomimetic drug of abuse, phencyclidine, on the acquisition and performance of a task sensitive to corticostriatal function was examined in nonhuman primates. METHODS: Monkeys were repeatedly exposed to phencyclidine (0.3 mg/kg) twice daily for 14 days. Acquisition and performance on an object-retrieval detour task was subsequently examined for up to 28 days after drug withdrawal. RESULTS: Animals treated with phencyclidine exhibited impaired acquisition of the task. The performance of trials requiring inhibitory control (as opposed to solely sensory-guided responding) was specifically impaired by prior phencyclidine administration. Impairments were found to be due to increased perseveration and barrier reaching. As is the case after frontal cortex ablation, the behavioral deficits were particularly evident during acquisition and appeared to be alleviated by prolonged training. CONCLUSIONS: The current data demonstrate that subchronic administration of phencyclidine can produce deficits in inhibitory response control that are manifest as impulsivity (increased control of behavior by unconditioned, appetitive stimuli). These data suggest that long-term phencyclidine exposure induces frontostriatal-like cognitive impairments and may represent a potential (drug induced) model for the study of prefrontal cortical cognitive and dopaminergic dysfunction.


Subject(s)
Cognition/drug effects , Hallucinogens/pharmacology , Impulsive Behavior/psychology , Phencyclidine/pharmacology , Animals , Chlorocebus aethiops , Conditioning, Operant/drug effects , Female , Male , Mental Recall/drug effects
19.
Neuropsychopharmacology ; 23(1): 79-88, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10869888

ABSTRACT

We have examined the effects of nicotine pre-treatment on mesoprefrontal dopamine (DA) function in the presence and absence of acute stress, and the involvement of endogenous opiate peptide systems (EOPS). Acute electrical footshock stress preferentially increases DA utilization in medial prefrontal cortex (mPFC) compared to nucleus accumbens (NAS) and striatal terminal fields, and this is correlated with profound locomotor immobility. Our recent studies have demonstrated that repeated, but not acute, nicotine pre-treatment significantly reduced mPFC DA utilization and footshock stress-induced immobility responses. There is increasing evidence that the biochemical and behavioral effects of nicotine are mediated by EOPS, and we hypothesized that the stress-reducing effects of repeated nicotine administration in these studies were mediated by EOPS. Accordingly, rats pre-treated subcutaneously with repeated nicotine were given a single dose of the opiate receptor antagonist naloxone (0.1-10.0 mg/kg, i.p.) or saline as a co-treatment with nicotine or saline 10 min prior to acute footshock stress. Naloxone had no effects on non-stressed or acute footshock stress-induced mPFC DA utilization, but dose-dependently antagonized repeated nicotine's attenuation of stress-induced mesoprefrontal DA utilization and immobility responses. Furthermore, naloxone dose-dependently blocked repeated nicotine's augmentation of accumbal DA utilization. These results suggest that EOPS may be involved in mediating repeated nicotine administration effects on mesoprefrontal dopaminergic and immobility responses to acute footshock stress.


Subject(s)
Dopamine/metabolism , Nicotine/pharmacology , Nicotinic Agonists/pharmacology , Prefrontal Cortex/drug effects , Stress, Physiological/metabolism , Animals , Immobilization/physiology , Male , Naloxone/pharmacology , Narcotic Antagonists/pharmacology , Opioid Peptides/drug effects , Opioid Peptides/metabolism , Prefrontal Cortex/metabolism , Rats
20.
Brain Res ; 864(1): 146-51, 2000 May 02.
Article in English | MEDLINE | ID: mdl-10793199

ABSTRACT

Predator odors may provide a species relevant aversive stimuli to study the central effects of stress in rats and may have several benefits over currently applied models. Here, we examined one such odor, TMT, isolated from the fox, a predator of the rat, on fear-induced behaviors, serum corticosterone, and central dopamine metabolism. Habituated rats were exposed to TMT, or a control odor, butyric acid, in an open field. For comparison, other rats were subjected to a model of conditioned fear - a traditional fear model. Several similarities between the two stresses were observed including increased serum corticosterone and increased dopamine metabolism in the medial prefrontal cortex. Differences were also observed. TMT, but not conditioned fear, activated dopamine metabolism in the amygdala, but not the nucleus accumbens core and shell. Rats exposed to conditioned fear, but not TMT odor, demonstrated altered behaviors associated with fear, including locomotion, grooming and immobility. Finally, rats reexposed to TMT after a 24-h delay did not demonstrate any of the changes observed with acute exposure to TMT. These data indicate acute exposure to a predator odor, TMT, can result in a unique pattern of biochemical activation that is similar, but not identical, to conditioned fear. The differences may indicate unique features of a central 'fear arousal' pathway that responds to innate, unlearned stressful stimuli, such as predator odors.


Subject(s)
Brain/drug effects , Brain/metabolism , Dopamine/metabolism , Neural Pathways/drug effects , Neural Pathways/metabolism , Neurons/drug effects , Neurons/physiology , Odorants , Predatory Behavior/drug effects , Predatory Behavior/physiology , Stress, Physiological/physiopathology , Thiazoles/pharmacology , Animals , Brain/cytology , Drug Administration Schedule , Fear/drug effects , Fear/physiology , Male , Mesencephalon/cytology , Mesencephalon/drug effects , Mesencephalon/metabolism , Neural Pathways/cytology , Neurons/chemistry , Nucleus Accumbens/cytology , Nucleus Accumbens/drug effects , Nucleus Accumbens/metabolism , Olfactory Pathways/cytology , Olfactory Pathways/drug effects , Olfactory Pathways/physiology , Prefrontal Cortex/cytology , Prefrontal Cortex/drug effects , Prefrontal Cortex/metabolism , Rats
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