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1.
Ned Tijdschr Geneeskd ; 1642020 10 15.
Article in Dutch | MEDLINE | ID: mdl-33201639

ABSTRACT

A 45-year-old man presented with acute intestinal obstruction, due to congenital peritoneal encapsulation. This rare malformation leads to an accessory peritoneal sac around all or part of the small bowel. Torsion of this sac can lead to acute bowel obstruction and therefore ischaemia. In this case, detorsion and adhesiolysis resulted in a positive outcome.


Subject(s)
Abdomen/pathology , Abdominal Pain/etiology , Congenital Abnormalities/pathology , Intestinal Obstruction/etiology , Intestine, Small/abnormalities , Nausea/etiology , Peritoneum/pathology , Acute Disease , Humans , Intestinal Obstruction/pathology , Intestinal Obstruction/therapy , Intestine, Small/pathology , Male , Middle Aged
2.
Biotechnol Prog ; 17(2): 336-47, 2001.
Article in English | MEDLINE | ID: mdl-11312712

ABSTRACT

Streptomyces clavuligerus is a commercially important actinomycete that is used to produce clavulanic acid, a beta-lactamase inhibitor. Observations during 10 batch cultivations with S. clavuligerus on defined media have led to the finding that the organism is very sensitive to shear when grown in batch cultures with increasing stirrer speed. The stirrer speed was increased to keep the dissolved oxygen level above 50% air saturation. A quantitative approach based on the calculation of elemental balances and a simple mathematical model is proposed to characterize the biomass lysis. Finally, a linear relation between biomass yield and observed specific growth rate is determined. Results show that cell lysis occurs at a high degradation rate, e.g., mu(max) = 0.16 h(-1) and k(d) = 0.07 h(-1), when the gassed power input increases above 1.1, 1.7, or 2.0 kW/m(3), respectively, depending on the medium composition. The overall biomass yield on substrate is dramatically reduced in all experiments (>30%).


Subject(s)
Streptomyces/cytology , Biomass , Culture Media , Models, Theoretical
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