ABSTRACT
The genetic polymorphism of the goat Kappa casein was investigated in Tunisian goats. Blood samples were collected from local goat breeds. Samples of genomic DNA were obtained from leukocytes of 175 dairy goats and regions of interest in the gene were amplified by Polymerase Chain Reaction (PCR) and then evaluated in agarose gel. For a better characterization of the single nucleotide polymorphism, a PCR-Restriction Fragment Length Polymorphism was performed employing the endonuclease DNA amplification using 459 bp primers. The PCR products of primers (459 bp) digested by restriction enzyme Alw44I produced two fragments of 459 and 381 bp. The Kappa casein allelic variants in tested animals revealed different genotypes, two of them were homozygous: AA or BB, AC or BC and CC. Genotypic frequencies were 12.5, 60.5 and 27% for AA or BB, CC and AC or BC, respectively. Identification of different variants of the Kappa casein can be used for the improvement and conservation of Tunisian local goats.
Subject(s)
Caseins/genetics , Goats/genetics , Polymorphism, Single Nucleotide , Animals , Electrophoresis, Agar Gel , Gene Frequency , Genotype , Polymerase Chain Reaction , TunisiaABSTRACT
The objective of this study was to determine the effects of grain compared to spineless cactus feeding on goat kids growth, carcass characteristics and FA profile. For this purpose, 21 kids were used and allocated to 3 groups receiving a low quantity (200g) of oat hay. The control group received ad libitum a concentrate containing 130g crude protein (CP) per kg of dry matter (C130). The second group received half of that contained consumed by the control one but its CP content was 260g/kg DM and spineless cactus ad libitum (C260-Cac). In the third group, concentrate intake was limited to soya bean at a quantity that provided the same CP quantity as the two other groups and also cached spineless cactus was distributed ad libitum (Soya-Cac). Animals of all groups had free access to water. At the end of the growth trial which lasted for 74 days, all kids were slaughtered. Samples of longissimus dorsi muscle were used for meat quality and FA composition study. Animals in the control group and those in the C260-Cac had higher growth rate than Soya-Cac diet animals. Muscle and adipose tissue mean weights were higher in the first groups while the bone weight was similar in all treatments. Animals given Soya-Cac diet had relatively less fat (10.5%) than those fed other diets (p<0.001). Carcass fat content tended to be lower (p=0.07) in C260-Cac goats (13.5%) than in those of the C130 group (15.8%). The ultimate pH ranged between 6.18 and 6.48; it was higher in meat from control goats (C130) than in animals receiving cactus. Dietary treatment had no significant effect (p>0.05) on meat moisture, ash, crude fat and protein contents. The intra muscular lipid composition in fatty acids showed differences between the control group and those receiving cactus. Cactus in the diet was associated with more C18:2 and conjugated linoleic acid (CLA) as well as a higher proportion of PUFA and PUFA:SFA ratio than control ones. In conclusion, this study showed that cactus feeding of goat kids maximises the proportion of CLA, PUFA and PUFA:SFA ratio.
ABSTRACT
Post-mortem spermatozoa recovery is an important technique for obtaining germplasm reserves from genetically valuable animals or endangered species. However, there are many factors that influence the outcome of this technique. We have studied the effect of the interval between animal's death and sperm recovery (0, 24 or 48 h) on the quality and freezability of ram spermatozoa from cauda epididymidis. Storage temperature of epididymis (room temperature or 5 degrees C) was also analysed. Spermatozoa were diluted with Tes-Tris-Fructose solution supplemented with egg yolk (10%) and glycerol (4%), and frozen using a programmable biofreezer (-20 degrees C/min). Pre-freeze and post-thaw sperm samples showed viable spermatozoa up to 48 h after the animal's death, although their quality declined significantly as post-mortem storage time increased. Epididymis sperm stored at 5 degrees C showed better motility and a lower percentage of abnormal forms than epididymis stored at room temperature after 24 and 48 h. The fertilizing ability of cauda epididymis ram spermatozoa obtained at 0 and 24h after the animal's death is similar to that of ejaculated spermatozoa. Therefore, a good protocol for post-mortem semen collection in rams when epididymal spermatozoa cannot be collected immediately, is to preserve the epididymis at 5 degrees C and process the samples in the first 24h after the animal's death.
