ABSTRACT
Kingella kingae strain KK247 was isolated from an adult Israeli patient with endocarditis. It belongs to pulsed-field gel electrophoresis clone A, has a 2,113,021-bp genome, a 15,507-bp plasmid that carries genes encoding ß-lactamases, and possesses 45 transposases, compared to the 5 detected in other K. kingae strains.
ABSTRACT
Coxiella burnetii is a pathogen causing Q fever. The aim of our work was to study Z3055, a strain that is genotypically related to the strain causing the Netherlands outbreak. We compared Z3055 to 5 other completed genomes available in GenBank. We calculated the blast score ratio (BSR) to analyze genetic differences among the strains. The ratio core genome/pangenome was 98% likely other bacteria with closed pangenomes. Differences between Z3055 and the reference NMI consisted only of point mutations and insertion/deletion (INDELs). Non-synonymous mutations significantly increased in genes coding for membrane proteins (16/156 vs 103/1757, bilateral Chi(2) test, p<0.05), ankyrin repeat domains containing proteins (2/9 vs 117/1904, bilateral Chi(2) test, p<0.05), transcription factors (7/53 vs 112/1860, bilateral Chi(2) test, p<0.05) and translation proteins (15/144 vs 109/1655, bilateral Chi(2) test, p<0.05). The evolution of this strain may have been driven by mutations in critical genes.
Subject(s)
Bacterial Proteins/genetics , Coxiella burnetii/genetics , Disease Outbreaks , Genetic Drift , Genome, Bacterial , Q Fever/epidemiology , Ankyrin Repeat/genetics , Bacterial Proteins/metabolism , Clone Cells , Coxiella burnetii/classification , Coxiella burnetii/pathogenicity , Genotype , Humans , INDEL Mutation , Membrane Proteins/genetics , Membrane Proteins/metabolism , Netherlands/epidemiology , Phylogeny , Point Mutation , Q Fever/microbiology , Q Fever/pathology , Transcription Factors/genetics , Transcription Factors/metabolismABSTRACT
Coxiella burnetii 109, with a 2.03-Mb genome, is a doxycycline-resistant human isolate that was isolated from the cardiac valve of a German male patient with Q fever endocarditis who died during the course of the treatment due to the bacterium's resistance to doxycycline. This new genome can be useful for future comparative genomic or Q fever studies.
Subject(s)
Coxiella burnetii/genetics , Doxycycline/pharmacology , Endocarditis, Bacterial/microbiology , Genome, Bacterial , Q Fever/microbiology , Anti-Bacterial Agents/therapeutic use , Coxiella burnetii/drug effects , Coxiella burnetii/isolation & purification , DNA, Bacterial/genetics , Drug Resistance, Bacterial/genetics , Endocarditis, Bacterial/drug therapy , Heart Valves/microbiology , Humans , Male , Molecular Sequence Data , Sequence Analysis, DNAABSTRACT
Kingella kingae is a betaproteobacterium from the order Neisseriales, and it is an agent of invasive infections in children. We sequenced the genome from the septic arthritis strain 11220434. It is composed of a 1,990,794-bp chromosome but no plasmid, and it contains 2,042 protein-coding genes and 52 RNA genes, including 3 rRNA genes.
