Chromium bioavailability in aquatic systems impacted by tannery wastewaters. Part 2: New insights from laboratory and in situ testing with Chironomus riparius Meigen (Diptera, Chironomidae).

Chromium is widely used as a tanning agent and can become a contaminant of concern in aquatic ecosystems receiving discharges from industrial or artisanal tanning activities. In a companion study, we showed that Cr discharged by tanneries was bioavailable to indigenous chironomids with accumulation via sediment ingestion likely to represent the predominant exposure route. However, Cr accumulation by chironomids did not directly reflect the degree of sediment contamination and the potential adverse effects of Cr accumulation on chironomids were not evaluated. In the present study, chironomids were exposed to homogenised, field-collected sediments in the laboratory and to intact sediments in situ using a customized caging system. Chromium concentrations were assessed in sediments, exposed larvae of laboratory-reared Chironomus riparius and overlying waters of in situ cages. Experimental results of Cr bioaccumulation were compared with expected Cr body burden in chironomids calculated using biodynamic modelling. Our data provided strong support to the hypothesis that Cr bioaccumulation in the field is specifically controlled by the deposition of contaminated suspended particulate matter (SPM) containing a pool of Cr readily bioavailable to surface deposit feeders. Considering freshly deposited SPM as an additional route of exposure for surface deposit feeders leads to a good agreement between the modelling and experimental results. Additionally, a Cr body burden of about 77 µg g-1 d.w. was identified as a tentative threshold above which effects on the growth of C. riparius may appear. While both laboratory and in situ experiments provide evidence for the availability of Cr in aquatic system impacted by tannery wastewaters, standard laboratory exposure conditions may miss additional exposure routes in the field and underestimate possible adverse effects on benthic organisms.

Chromium bioavailability in aquatic systems impacted by tannery wastewaters. Part 1: Understanding chromium accumulation by indigenous chironomids.

The tanning industry uses large quantities of Cr whose contribution to the contaminant burden of aquatic organisms is not yet fully understood. The present study investigated Cr bioaccumulation by indigenous chironomids in a freshwater ecosystem impacted by tannery effluents. Total Cr content in sediments and in chironomids was determined on several occasions. Chromium distribution among sediments and pore waters, and Cr speciation in overlying and pore waters were studied in detail to understand possible factors controlling Cr bioavailability to chironomids. Total chromium concentration ranged from 69 to over 3000 µg g-1 dry weight in sediments and from negligible to over 300 µg g-1 dry weight in chironomids (values corrected for sediment gut content). Filterable (<0.45 µm) Cr concentration in overlying waters and pore waters from the surface sediment layers (upper 2 cm) ranged from 3 to 120 µg L-1, with Cr(VI) representing 0.5-28% of the total filterable Cr. Chromium profiles in pore waters as determined by diffusive equilibration in thin films (DET) and diffusive gradient in thin films (DGT) were comparable. DGT-labile Cr accounted for <2% of the total Cr measured by DET. Although Cr concentrations in sedimentary and aqueous matrices were not directly proportional to Cr levels measured in chironomids, the available findings suggested that Cr inputs from tanneries were bioavailable to resident chironomids. These observations are of particular importance considering that Cr(III), putatively of limited bioavailability and ecotoxicological concern, is the predominant redox form of Cr in bed sediments impacted by tannery discharges. The companion paper provides further insight into Cr bioavailability and effects in tannery impacted ecosystems using a combination of in situ and laboratory approaches.

Measurement of dynamic mobilization of trace metals in sediments using DGT and comparison with bioaccumulation in Chironomus riparius: first results of an experimental study.

Sediments in aquatic ecosystems are often contaminated as a result of anthropogenic activities. Sediments and benthic organisms have been used to monitor trace metals contamination. However, due to the high variability of contaminant bioavailability, the attempt to link metal concentration in sediments and contamination of the organisms or ecotoxicological effect often lead to disappointing results. The technique of diffusive gradients in thin films (DGT) has been proposed as a relevant tool to study metal bioavailability, for example for accumulation in plants. In the present study, laboratory microcosm experiments were conducted with six contaminated sediments to compare metal accumulation in DGT and bioaccumulation in a chironomid (Chironomus riparius) for Cu, Cd and Pb . Metal accumulation in DGT was measured over time then modelled to determine two parameters of the dynamic response of the metals to DGT deployment: the size of the particulate labile pool and the kinetic of the solid-dissolved phase exchange. The mobility of metals was found metal and sediment dependent. A significant relationship between metal accumulated in DGT and bioaccumulated in chironomids was found for Cu and Pb. However, total metals in sediments were the best predictors of bioaccumulation. Nevertheless, the knowledge of the metals dynamic enhanced our ability to explain the different biological uptake observed in sediments of similar total metal concentrations.