Plasma glutamine concentrations in the horse following feeding and oral glutamine supplementation.

REASONS FOR PERFORMING STUDY: Pharmacological benefits of glutamine supplementation have been shown in athletically and clinically stressed human subjects. In the horse, infection and intense exercise have also been shown to significantly decrease plasma glutamine concentrations, but little is known on how best to supplement. OBJECTIVE: To evaluate whether ingestion of different foodstuffs, with or without L-glutamine (G) or a peptide (Pep) containing 31.5% w/w G in a water-stable form, could affect plasma glutamine concentrations (P-GC). MATERIALS AND METHODS: Nine feeds (molassed sugar beet-pulp (mSB); naked oats (nO); commercial mix (CM); mSB with 30 or 60 mg/kg bwt G or the G-molar equivalent of Pep; and CM with 60 mg/kg bwt G or equivalent Pep) were offered to 6 healthy mature horses on different days following overnight food restriction. The changes in P-GC were monitored for 8 h post feeding. RESULTS: After 1.5 h mean +/- s.d. AP-GC were -0.9 +/- 10.2% (mSB), +12.5 +/- 7.1% (nO) and +44.7 +/- 15.9% (CM; P<0.05). deltaP-GC with mSB supplemented with G was +60.9 +/- 30.0% (30 mg; P<0.05) and +156.8 +/- 34.6% (60 mg; P<0.05) at 1 h; deltaP-GC with Pep was 51.0 +/- 31.0% (30 mg equivalent, P<0.05) and +91.1 +/- 9.5% (60 mg equivalent, P<0.05) at 1 h. After 10 days of supplementation with 60 mg/kg bwt G, AP-GC following a further 60 mg/kg bwt G challenge showed a similar increase at 1 h of +154.3 +/- 37.9%; prevalues were unchanged. G and Pep added to CM, increased P-GC by 246.3 +/- 55.3 (+99.2%) and 252.3 +/- 94.2 micromol/l (96.7%) at 1.5 h with concentrations still above prevalues at 8 h (P<0.05). Apart from the CM (with or without supplement), pre P-GC was always regained by 4 h. Plasma NH3 and plasma protein concentrations were unaffected by supplementation with G or Pep. CONCLUSION: P-GC may be modified by appropriate supplementation with no apparent adverse effects. POTENTIAL RELEVANCE: Increasing P-GC through appropriate supplementation may be of benefit in the athletically or clinically stressed horse with lowered plasma glutamine concentrations.

Plasma glutamine status in the equine at rest, during exercise and following viral challenge.

The variation over 24 h of plasma glutamine concentration in nonexercising horses was studied in 3 Thoroughbreds (TB) fed at 1600 h and 0700 h. This indicated a small but regular change associated with feeding. Starting at a mean of 482 mumol/l at 1600 h the concentration increased to 522 mumol/l at 2000 h, falling to 476 mumol/l at 1600 h and increasing again to 525 mumol/l at 2000 h. 'Normal' values were established in 19 part-bred TB horses, lacking clinical signs or remarkable pathology and in light training, by sampling weekly at 1000 h over a 10 week period. The mean concentration was 491 mumol/l. Values were normally distributed with 95% confidence range between horses of 469-512 mumol/l. The s.d. of values within-horse was 28 mumol/l. The acute effect of exercise was investigated in 5 TB horses during i) an exercise simulating the road and tracks phase of a 3-day-event and ii) a sustained high-intensity exercise test (115% VO2max until the pace was no longer maintained). In both tests a transient increase was produced as a direct result of exercise, followed by a decline to a nadir at approximately 3 h post exercise. Pre-exercise resting concentrations, following either test, were approaching pre-values by 24 h recovery. The effect of viral challenge was studied in 6 TB horses exposed to an aerosol of equine influenza virus, subtype H3N8 (A/equine-2 subtype) which caused mild clinical signs. A significant fall in the 1000 h plasma glutamine concentration from a mean of 463 to 342 mumol/l by the 6th day was seen in 5 horses. A more gradual decline was observed in the 6th horse. These findings indicate a similar response to viral challenge in the horse to that in man.