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Chem Commun (Camb) ; 50(89): 13746-9, 2014 Nov 18.
Article in English | MEDLINE | ID: mdl-25251465

ABSTRACT

Covalent "click" cycloaddition was used to functionalize silica substrates with pH-sensitive nanoparticles, thus producing uniform and highly luminescent analytical devices usable in both commercial fluorimeters and fluorescence microscopes. Quantitative and spatially-resolved extracellular pH measurements were successfully achieved on live cardiac fibroblasts with these novel ion-sensitive surfaces.


Subject(s)
Extracellular Space/chemistry , Nanoparticles/chemistry , Silicon Dioxide/chemistry , Silver/chemistry , Click Chemistry , Fibroblasts , Fluorescein-5-isothiocyanate/chemistry , Fluorescent Dyes/chemistry , Green Fluorescent Proteins/chemistry , Green Fluorescent Proteins/genetics , Humans , Hydrogen-Ion Concentration , Microscopy, Fluorescence , Myocardium/cytology
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