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1.
Vet Rec ; 176(24): 625, 2015 Jun 13.
Article in English | MEDLINE | ID: mdl-25861824

ABSTRACT

The object of the study was to quantify the prevalence of failure of passive transfer in UK dairy farms and to identify variables that had a significant impact on the rate of immunological transfer. In a six-month study of 444 calvings from seven UK dairy farms, 26 per cent of calves failed to receive adequate immunoglobulin transfer as judged by a plasma total protein (pTP) concentration less than 5.6 g/dl. Colostrum immunoglobulin concentration, indirectly measured using Brix refractometry, showed wide variations with samples ranging from 10.3 to 34.7 Brix units. Thirty-seven per cent of samples were below the suggested cut-off Brix score for colostrum quality of 22 per cent. Potential associations between covariates and plasma protein concentration were investigated using multiple linear regression models. The covariate with the greatest impact on the pTP concentration was the farm on which the calf was born (P<0.05). A significant but small association was demonstrated between colostrum immunoglobulin concentration and calf pTP concentration (P<0.01). Multiple linear regression models suggested that the time of colostrum collection after calving, parity of the dam, and the individual farm were associated with the Brix measurements (P<0.05). This study suggested that veterinary review of colostrum protocols on farm with emphasis on prompt collection and dosing after calving remains a simple and effective measure to improve passive transfer and thus calf health on UK dairy farms.


Subject(s)
Animals, Newborn/immunology , Blood Proteins/analysis , Colostrum/immunology , Immunoglobulin G/analysis , Maternal-Fetal Exchange , Animals , Cattle , Female , Pregnancy , United Kingdom
3.
Vet Rec ; 173(18): 450, 2013 Nov 09.
Article in English | MEDLINE | ID: mdl-24128945

ABSTRACT

Plasma progesterone concentrations were estimated for 381 nulliparous dairy heifers from eight farms on days 4-6 after insemination. Heifers were synchronised using two treatments of prostaglandin F2α (PGF2α) 11 days apart. Alternate heifers were treated with 1500 IU human chorionic gonadotrophin (hCG) at the time of sampling, and the pregnancy status assessed ~35 days after insemination. Heifers with no corpus luteum (CL; n-30) at the second PGF2α had lower progesterone concentrations (P<0.001) and pregnancy rates (PR; P=0.001) compared with heifers with CL (n=351, defined as cyclic). The risk of no CL increased in heifers <15 months of age (OR=7.5, P<0.001) and with body condition score <2.5 (OR=4.5, P=0.001). Those with no CL were removed from further analysis. The 20 per cent cyclic control heifers with progesterone concentrations ≤2 ng/ml on days 4-6 had lower PRs compared with those with higher concentrations (42 per cent vs 64 per cent, OR=0.3, P=0.01). There was no overall effect of hCG on PR compared with controls (51 per cent vs 60 per cent, P=0.10). However, heifers with high progesterone on days 4-6 (>2 ng/ml) had a lower PR after treatment (51 per cent vs 64 per cent, OR=0.6, P=0.02). Heifers inseminated with sexed (n=18) compared with conventional (n=333) semen had a decreased PR (33 per cent vs 57 per cent, OR=0.2, P=0.01). This study demonstrates that higher plasma progesterone concentrations as early as days 4-6 after insemination have a positive effect on first insemination PR, but there was no beneficial effect of hCG treatment on these days in dairy heifers.


Subject(s)
Cattle/physiology , Chorionic Gonadotropin/pharmacology , Chorionic Gonadotropin/therapeutic use , Fertilization/drug effects , Insemination, Artificial/veterinary , Pregnancy Rate , Progesterone/blood , Animals , Cattle/blood , Dinoprost/administration & dosage , Estrus/physiology , Estrus Synchronization/methods , Female , Parity , Pregnancy , Random Allocation , Time Factors , Treatment Outcome
4.
J Dairy Sci ; 93(5): 2239-43, 2010 May.
Article in English | MEDLINE | ID: mdl-20412939

ABSTRACT

The purpose of this study was to estimate and discuss the genetic variation, heritability, and effects of nongenetic factors on the ability of Holstein-Friesian cows to produce an immune response by producing IgG antibodies to Ostertagia ostertagi. Total IgG (IgG(1) and IgG(2)) antibody levels were determined using an ELISA and measured using optical density ratio (ODR=OD(sample) - OD(negative control)/OD(positive control) - OD(negative control)) from milk samples collected from 1,276 Holstein-Friesian cattle in 229 commercial dairy farms from 2002 to 2004 during their first (82%) and other (2 to 12) lactations. A sire (n=461) model was fitted to the ODR data using ASREML software, and variance components were estimated. The ability to produce O. ostertagi antibodies as measured by ODR had a heritability of 0.13+/-0.12, and both season of sample and herd had a significant effect on total IgG levels. To conclude, this study has ascertained that genetic variation is present in the ability of dairy cows to mount an immune response to the parasite O. ostertagi. Inasmuch as evidence exists that IgG is linked to protective immunity against the parasite via a reduction in its reproductive ability, this trait may be of potential interest to genetic selection programs as an aid to reduce the effect of O. ostertagi in dairy herds.


Subject(s)
Antibodies, Helminth/analysis , Cattle Diseases/genetics , Cattle Diseases/immunology , Milk/immunology , Ostertagiasis/veterinary , Animals , Cattle , Cattle Diseases/blood , Genetic Variation , Immunoglobulin G/analysis , Ostertagia/immunology , Ostertagiasis/genetics , Ostertagiasis/immunology , Quantitative Trait, Heritable , Seasons , United Kingdom
5.
J Dairy Sci ; 93(4): 1743-8, 2010 Apr.
Article in English | MEDLINE | ID: mdl-20338453

ABSTRACT

Activity of stearoyl-CoA desaturase (SCD) in the mammary gland is important for determining the relative proportions of saturated and monounsaturated fatty acids in milk and the concentration of the conjugated linoleic acid isomer rumenic acid (RA; cis-9,trans-11 18:2). Previous studies identified a large degree of between-cow variation in SCD activity, which was consistent across diets and suggests a genetic influence. The objectives of this study were to quantify genetic and phenotypic variations in fatty acid concentrations and SCD indices in milk fat and to estimate their heritabilities in a population of United Kingdom dairy cows. Milk samples were collected from 2,408 daughters of 597 Holstein-Friesian sires on 325 commercial farms for determination of fatty acid profiles. Indices of SCD activity were calculated by expressing each SCD product (cis-9 14:1, cis-9 16:1, cis-9 18:1, and RA) as a proportion of the precursor plus product [e.g., SCDI(14)=cis-9 14:1/(14:0+cis-9 14:1)]. For individual fatty acids, phenotypic variance was considerably greater than additive genetic variance, resulting in small and nonsignificant heritability estimates (+/- standard error) for all except 6:0 (h(2)=0.27+/-0.10), 8:0 (h(2)=0.27+/-0.09), 12:0 (h(2)=0.13+/-0.07), cis-9 14:1 (h(2)=0.28+/-0.10), and cis-9 18:1 (h(2)=0.12+/-0.07). Heritability estimates of desaturase indices were significant for SCDI(14) (h(2)=0.38+/-0.11), SCDI(18) (h(2)=0.19+/-0.09), and SCDI(RA) (h(2)=0.21+/-0.09), but not for SCDI(16) (h(2)=0.05+/-0.06). This study provides evidence that additive effects are responsible for a significant proportion of the phenotypic variation in SCD activity in dairy cows. It is concluded that because heritability of desaturase indices is moderate and significant in many cases, these indices could be investigated further for use in future breeding programs to increase concentrations of monounsaturated fatty acids and RA while decreasing concentrations of saturated fatty acids in milk fat.


Subject(s)
Cattle/genetics , Fatty Acids/analysis , Lactation/genetics , Milk/chemistry , Stearoyl-CoA Desaturase/metabolism , Animals , Breeding , Cattle/metabolism , Female , Genetic Variation , Mammary Glands, Animal/enzymology , Stearoyl-CoA Desaturase/genetics
6.
Anim Genet ; 41(3): 329-31, 2010 Jun.
Article in English | MEDLINE | ID: mdl-19968639

ABSTRACT

Seven sequence variants (SVs) have been identified in exon 1 and in the promoter region upstream of the bovine gonadotrophin releasing hormone (GnRH) receptor gene, at nucleotides g.-331A>G, g.-108T>C, g.+206G>A, g.+260C>T, g.+341C>T, g.+383C>T and g.+410C>T relative to the translation start site. The SVs at nucleotides g.-108, g.260, g.341 and g.410 and those at g.206 and g.383 formed two groups with complete linkage disequilibrium within groups, but incomplete linkage disequilibrium between groups, and none of the SVs altered receptor amino acid sequence. The g.-108T>C allelic variants were associated with an approximately 0.4 day reduction in predicted transmitting ability for days to first service. None of the allelic variants affected the pattern of circulating LH following administration of GnRH. The g.260C>T alteration introduced a new transcription factor binding site in a region of DNA with relatively low nucleosome formation potential. The data suggest that selection for animals carrying the g.-108T>C group of alterations will improve fertility in the dairy cow.


Subject(s)
Cattle/genetics , Fertility , Receptors, LHRH/genetics , Animals , Female , Luteinizing Hormone/genetics , Male
7.
Vet Rec ; 165(4): 102-6, 2009 Jul 25.
Article in English | MEDLINE | ID: mdl-19633322

ABSTRACT

A total of 220 cows and heifers were treated with 3 mg/kg ketoprofen immediately after calving and 24 hours later, and 227 were left untreated as controls. The treated animals tended to have a lower incidence of retained fetal membranes and were 1.7 times less likely to develop the condition than the untreated animals. The treatment did not affect the incidence of milk fever, the endometritis score or the presence of a corpus luteum by 20 to 25 days after calving, and did not affect the cows' early lactation milk yield. There was no particular level of dystocia or periparturient conditions other than retained fetal membranes for which there might be a reproductive or productive advantage following the use of ketoprofen. The intervals from calving to first insemination or to pregnancy, the numbers of inseminations per pregnancy and the pregnancy rate to first insemination were also unaffected by ketoprofen treatment.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Cattle Diseases/chemically induced , Fertility/drug effects , Ketoprofen/pharmacology , Lactation/drug effects , Animals , Cattle , Cattle Diseases/drug therapy , Cattle Diseases/epidemiology , Dystocia/drug therapy , Dystocia/veterinary , Endometriosis/chemically induced , Endometriosis/epidemiology , Endometriosis/veterinary , Female , Milk/drug effects , Milk/metabolism , Placenta, Retained/chemically induced , Placenta, Retained/epidemiology , Placenta, Retained/veterinary , Postpartum Period , Pregnancy , Survival Analysis , United Kingdom/epidemiology
8.
J Dairy Sci ; 92(8): 4001-7, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19620683

ABSTRACT

The decline of dairy cattle fertility worldwide remains a major concern, with conception rates to first service commonly below 40%. The length and severity of negative energy balance postpartum are unfavorably correlated with fertility, suggesting that the length and severity of negative energy balance and fertility are linked via several hormones or metabolites. These compounds therefore have the potential to predict fertility at a genetic level. The addition of a predictor trait for fertility into present fertility indices would accelerate genetic gain, particularly if it was expressed before adulthood. The objective of this work was to estimate the genetic variation in several metabolites and hormones in calves, and to determine their genetic relationships with fertility and production through sire predicted transmitting abilities (PTA; sires of calves sampled). Circulating concentrations of free fatty acids (FFA), glucose, growth hormone (GH), insulin, and insulin-like growth factor 1 (IGF-1) in male and female UK Holstein-Friesian dairy calves (average age +/- SD; 126 +/- 12.7 d) were analyzed during 2 studies: data set 1 (n = 496 females; 1996-2001; 7 commercial dairy herds) and data set 2 (n = 326 females, n = 256 males; 2002-2006; multiple ovulation and embryo transfer breeding scheme). Univariate mixed models were fitted to the data using ASREML. Basal concentrations of FFA, glucose, GH, insulin and total IGF-1 were all moderately heritable in both sexes (heritability range +/- SE; 0.09 +/- 0.05 to 0.66 +/- 0.14). The sire PTA for protein percentage had significant regression coefficients and approximate genetic correlations with FFA and insulin, and the sire PTA for calving interval had significant regression coefficients and approximate genetic correlations with GH. Additive genetic variance seems responsible for a moderate proportion of the phenotypic variation in important metabolites and regulatory hormones in male and female UK Holstein-Friesian dairy calves, therefore supporting further investigation into their use as juvenile predictors for fertility in the mature female.


Subject(s)
Blood Glucose/analysis , Cattle , Fatty Acids, Nonesterified/blood , Genetic Variation , Growth Hormone/blood , Insulin-Like Growth Factor I/analysis , Insulin/blood , Animals , Cattle/blood , Cattle/genetics , Dairying/economics , Female , Male
9.
Prev Vet Med ; 86(1-2): 153-63, 2008 Aug 15.
Article in English | MEDLINE | ID: mdl-18495277

ABSTRACT

We examined whether infrequent milk sampling for progesterone analysis could be used as a management or diagnostic tool by the dairy farmer to predict delayed ovarian cyclicity in dairy cows. The data included 1040 lactations from 324 Swedish Red cows and 183 Swedish Holstein cows and were randomly divided into two datasets. A logistic regression model was fit to the first dataset and the model was then validated on the other dataset. The model was also validated using a British dataset comprising 1212 lactations from 1080 British Holstein-Friesian cows. The dependent variable was whether delayed ovarian cyclicity occurred or not, delayed ovarian cyclicity defined as progesterone levels below threshold value for the first 56 or 45 days postpartum in the Swedish or British dataset, respectively. The basic model included the effects of breed, parity, season and housing type. To the basic model various progesterone-based measurements were added. These were the interval from calving to commencement of luteal activity and the percentage of samples with luteal activity within 60 days after calving, using all samples in the databases or one sample per month. The accuracy of the conditional probability of delayed ovarian cyclicity calculated with the different models was obtained using receiver operating characteristic (ROC) curves and calculating the area under curve. Sensitivity and specificity were calculated for cut-off probabilities using the ROC analyses. The accuracy was highest (0.94-0.99) when including the progesterone measurements based on milk sampling for progesterone analysis 2-3 times per week. The accuracy was between 0.85 and 0.88 when the progesterone measurements with monthly milk sampling were added to the model and 0.76 or 0.67 with the basic model. This study clearly shows that infrequent milk sampling for progesterone analysis, such as once a month as in the regular milk recording system, could be used to predict delayed ovarian cyclicity in dairy cows. This increases the opportunity for earlier treatment of anovulatory dairy cows and may therefore decrease involuntarily extended calving intervals in the herd.


Subject(s)
Cattle/physiology , Estrous Cycle/physiology , Milk/chemistry , Progesterone/analysis , Animals , Female , Fertility/physiology , Logistic Models , Ovulation/physiology , Parity , Predictive Value of Tests , Pregnancy , ROC Curve , Random Allocation , Sensitivity and Specificity , Time Factors
10.
Animal ; 2(8): 1101-3, 2008 Aug.
Article in English | MEDLINE | ID: mdl-22443721
11.
J Dairy Sci ; 90(8): 3909-16, 2007 Aug.
Article in English | MEDLINE | ID: mdl-17639002

ABSTRACT

The objective of this work was to estimate the genetic variation of free fatty acids (FFA), glucose, growth hormone (GH), and insulin in juvenile male dairy calves and to assess the relationships, if present, with the fertility of their female offspring. This study used data from 1,498 (269.5 d of age +/- 11.1) male calves from a multiple ovulation and embryo transfer breeding scheme (data collected from 1997 to 2002). Calves were Danish Holstein (n = 1,047), Danish Jersey (n = 200), and Red Dane (n = 251), and were sampled following an overnight fast at approximately 9 mo of age. Plasma samples were assayed for basal FFA, glucose, GH, and insulin. Estimated breeding values of female fertility (high values indicating better fertility), based on progeny-test results for approximately 100 daughters per sire, were available for a subset (n = 810) of the male calves as adult sires. Data from Danish Holstein alone or Danish Holstein, Red Dane, and Danish Jersey combined (all breeds) were analyzed for each trait. In both data sets, the estimates of heritabilities of glucose (0.27 +/- 0.06), FFA (0.11 +/- 0.05), and insulin (0.21 +/- 0.06) were moderate, and that of GH (0.09 +/- 0.05) was low. Correlations of estimated breeding values for fertility traits with glucose and FFA breeding values were negative, indicating that male calves with high glucose or FFA had female offspring with reduced fertility. Selection for bull calves with lower concentrations of glucose and FFA following an overnight fast could result in female offspring with genetically better fertility. Glucose and FFA may therefore be of interest to enhance selection for improved female fertility, as a measurement in young bulls.


Subject(s)
Cattle/metabolism , Fertility/physiology , Genetic Variation , Models, Statistical , Animals , Blood Glucose/analysis , Blood Glucose/genetics , Blood Glucose/physiology , Breeding , Cattle/genetics , Cattle/physiology , Cohort Studies , Dairying , Fatty Acids, Nonesterified/blood , Fatty Acids, Nonesterified/genetics , Fatty Acids, Nonesterified/physiology , Female , Fertility/genetics , Growth Hormone/blood , Growth Hormone/genetics , Growth Hormone/physiology , Insulin/blood , Insulin/genetics , Insulin/physiology , Male , Models, Biological , Phenotype , Statistics as Topic
12.
J Dairy Sci ; 90(1): 427-34, 2007 Jan.
Article in English | MEDLINE | ID: mdl-17183111

ABSTRACT

The objective of this study was to estimate genetic parameters for measures of luteal activity during the first 60 d postpartum. Analyses were made with different sampling intervals to investigate the possibility of combining progesterone measurement with routinely performed milk recording. Progesterone level in milk as an indicator of female fertility when selecting sires in a progeny-testing scheme was also examined. Data were collected from 1996 to 1999, and comprised 1,212 lactations from 1,080 British Holstein-Friesian cows at 8 commercial dairy farms in the United Kingdom. Milk samples for progesterone analysis were collected thrice weekly. Mixed linear animal models were used to analyze the data. Heritability for the percentage of samples with luteal activity during the first 60 d postpartum (PLA) was 0.30 and decreased with more infrequent sampling to 0.25, 0.20, and 0.14 for weekly, twice-monthly, and monthly sampling, respectively. Measures of PLA had a high negative genetic correlation with prolonged anovulation (-0.53 for monthly sampling, < -0.87 otherwise) and a moderate positive genetic correlation with persistent corpus luteum in the first estrus cycle (>0.65 if at least twice-monthly sampling). Genetic correlations with interval from calving to commencement of luteal activity were close to -1 for all PLA measurements and the selection index calculations showed that monthly progesterone sampling could be used with high accuracy (0.80 with 50 daughters per bull) to predict breeding values for commencement of luteal activity. Progesterone analysis at the time of regular milk recording could thereby be used to select for an early interval from calving to commencement of luteal activity and, at the same time, a decreased frequency of prolonged anovulation during the postpartum period.


Subject(s)
Corpus Luteum/metabolism , Genetic Variation , Postpartum Period , Animals , Breeding , Cattle , Dairying , Female , Fertility/genetics , Lactation , Male , Milk/chemistry , Milk/metabolism , Phenotype , Progesterone/genetics , Progesterone/metabolism
13.
J Dairy Sci ; 85(11): 3071-80, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12487474

ABSTRACT

The decline of fertility in the UK dairy herd and the unfavorable genetic correlation (r(a)) between fertility and milk yield has necessitated the broadening of breeding goals to include fertility. The coefficient of genetic variation present in fertility is of similar magnitude to that present in production traits; however, traditional measurements of fertility (such as calving interval, days open, nonreturn rate) have low heritability (h2 < 0.05), and recording is often poor, hindering identification of genetically superior animals. An alternative approach is to use endocrine measurements of fertility such as interval to commencement of luteal activity postpartum (CLA), which has a higher h2 (0.16 to 0.23) and is free from management bias. Although CLA has favorable phenotypic correlations with traditional measures of fertility, if it is to be used in a selection index, the genetic correlation (ra) of this trait with fertility and other components of the index must be estimated. The aim of the analyses reported here was to obtain information on the ra between lnCLA and calving interval (CI), average body condition score (BCS; one to nine, an indicator of energy balance estimated from records taken at different months of lactation), production and a number of linear type traits. Genetic models were fitted using ASREML, and r(a) were inferred from genetic regression of lnCLA on sire-predicted transmitting abilities (PTA) for the trait concerned by multiplying the regression coefficient (b) by the ratio of the genetic standard deviations. The inferred r(a) between lnCLA and CI and average BCS were 0.36 and -0.84, respectively. Genetic correlations between InCLA and milk fat and protein yields were all positive and ranged between 0.33 and 0.69. Genetic correlations between InCLA and linear type traits reflecting body structure ranged from -0.25 to 0.15, and between udder characteristics they ranged from -0.16 to 0.05. Thus, incorporation of endocrine parameters of fertility, such as CIA, into a fertility index may offer the potential to improve the accuracy of breeding value prediction for fertility, thus allowing producers to make more informed selection decisions.


Subject(s)
Cattle/genetics , Cattle/physiology , Corpus Luteum/physiology , Estrus/genetics , Fertility/genetics , Lactation/genetics , Animals , Body Constitution/genetics , Breeding , Dairying/methods , Female , Genetic Variation , Male , Milk , Models, Genetic , Selection, Genetic , United Kingdom
14.
J Dairy Sci ; 85(4): 958-67, 2002 Apr.
Article in English | MEDLINE | ID: mdl-12018442

ABSTRACT

The objective of this study was to estimate the heritability of a number of traditional and endocrine fertility traits in addition to d-56 predicted milk yield (MY56), and the genetic and phenotypic correlations between these traits. Various fixed effects such as season, year, herd, lactation number, diet, percentage Holstein (PCH) of the cow, and occurrence of uterine infection (UI), dystocia (DYS), and retained placenta (RP) were also investigated. Data collected for 1212 lactations of 1080 postpartum (PP) Holstein-Friesian dairy cows in eight commercial farms between 1996 and 1999 included thrice weekly milk progesterone samples, calving and insemination dates, various reproductive health records, monthly/bimonthly production records, three-generation pedigrees, and PCH information. Genetic models were fitted to the data to obtain heritabilitites and correlations using ASREML. Estimates of heritability for interval to commencement of luteal activity PP (lnCLA), length of the first luteal phase PP (lnLutI) and occurrence of persistent CL type I (PCLI) were 0.16, 0.17, and 0.13, respectively. Heritabilities for pregnancy to first service (PFS), interval to first service (IFS), and MY56 were 0.14, 0.13, and 0.50, respectively. Genetic regressions of lnCLA and lnLutI on PTA of the sire for milk, fat, and protein yields, and PIN95 were investigated. Regressions of lnCLA were positive and significant on fat yield, while regressions of lnLutI on both protein yield and PIN95 were negative and significant. Genetic correlations of endocrine fertility traits (lnCLA, lnLutI, and PCLI) with MY56 were high (0.36, P < 0.05; -0.51, P < 0.05; and -0.31, P < 0.1, respectively). Percentage Holstein of the cows had no significant effect on any of the fertility parameters monitored. This work emphasizes the strong genetic correlation of fertility with production traits and, therefore, highlights the urgent requirement for selective breeding for fertility in the United Kingdom. The high heritability of endocrine fertility traits stress their potential value for inclusion in a selection index to improve fertility.


Subject(s)
Cattle/physiology , Fertility/genetics , Lactation/genetics , Progesterone/analysis , Reproduction/genetics , Animals , Breeding , Cattle/genetics , Female , Genetic Variation , Health Status , Milk/chemistry , Milk/metabolism , Phenotype , Pregnancy
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