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1.
J Sports Sci Med ; 22(3): 591-596, 2023 09.
Article in English | MEDLINE | ID: mdl-37711709

ABSTRACT

Since the implementation of the US Soccer heading guidelines released in 2015, little to no research on ball-head impact exposure in the United States youth soccer population has been conducted. The purpose was to compare ball-head impact exposure across sex and age in youth soccer players over a weekend tournament. Ten male and female games for each age group (Under-12 [U12], U13, and U14) were video recorded at a weekend tournament for a total of 60 games. Ball-head impact exposure for each game was then coded following a review of each recording. Male players were 2.8 times more likely to have ball-head impacts than female players, (p < 0.001) particularly in the U14 age group when compared to the U12 age group (p = 0.012). Overall 92.4% of players experienced 0-1 ball-head impacts per game with the remaining players experiencing 2+ ball-head impacts per game. Ball-head impact exposure levels are low in the youth players. Most youth soccer players do not head the soccer ball during match play and those that did, only headed the ball on average once per game. Overall, the difference in ball-head impact exposure per player was less than 1 between all the groups, which may have no clinical meaning.


Subject(s)
Head , Soccer , Adolescent , Female , Humans , Male , Head Injuries, Closed , Athletic Injuries
2.
Sci Total Environ ; 801: 149501, 2021 Dec 20.
Article in English | MEDLINE | ID: mdl-34438141

ABSTRACT

Excess phosphorus (P) from agriculture is a leading cause of harmful and nuisance algal blooms in many freshwater ecosystems. Throughout much of the midwestern United States, extensive networks of subsurface tile drains remove excess water from fields and allow for productive agriculture. This enhanced drainage also facilitates the transport of P, particularly soluble reactive phosphorus (SRP), to adjacent streams and ditches, with harmful consequences. Thus, reducing SRP loss from tile-drained cropland is a major focus of regional and national efforts to curb eutrophication and algal blooms. The planting of cover crops after crop harvest is a conservation practice that has the potential to increase retention of fertilizer nutrients in watersheds by extending the growing season and limiting bare ground in the fallow season; however, the effect of cover crops on SRP loss is inconsistent at the field-scale and unknown at the watershed-scale. In this study, we conducted a large-scale manipulation of land cover in a small, agricultural watershed by planting cover crops on >60% of croppable acres for six years and examining changes in SRP loss through tile drains and at the watershed outlet. We found reduced median SRP loss from tiles with cover crops compared to those without cover crops, particularly during periods of critical export from January to June. Variation in tile discharge influenced SRP loss, but relationships were generally weaker in tiles with cover crops (i.e., decoupled) compared to tiles without cover crops. At the watershed outlet, SRP yield was highly variable over all seasons and years, which complicated efforts to detect a significant effect of changing land cover on SRP export to downstream systems. Yet, watershed-scale planting of cover crops slowed cumulative SRP losses and reduced SRP export during extreme events. Overall, this study demonstrates the potential for cover crops to alter patterns of SRP loss at both the field- and watershed-scale.


Subject(s)
Ecosystem , Phosphorus , Agriculture , Crops, Agricultural , Fertilizers
3.
Science ; 372(6548): 1272, 2021 Jun 18.
Article in English | MEDLINE | ID: mdl-34140376
4.
J Athl Train ; 56(4): 408-417, 2021 Apr 01.
Article in English | MEDLINE | ID: mdl-33878174

ABSTRACT

CONTEXT: Approximately 72% of patients with an ankle sprain report residual symptoms 6 to 18 months later. Although 44% of patients return to activity in less than 24 hours after experiencing a sprain, residual symptoms should be evaluated in the long term to determine if deficits exist. These residual symptoms may be due to the quality of ligament tissue and motion after injury. OBJECTIVE: To compare mechanical laxity of the talocrural joint and dorsiflexion range of motion (DFROM) over time (24 to 72 hours, 2 to 4 weeks, and 6 months) after an acute lateral ankle sprain (LAS). DESIGN: Cross-sectional study. SETTING: Athletic training research laboratory. PATIENTS OR OTHER PARTICIPANTS: A total of 108 volunteers were recruited. Fifty-five participants had an acute LAS and 53 participants were control individuals without a history of LAS. MAIN OUTCOME MEASURE(S): Mechanical laxity (talofibular interval and anterior talofibular ligament length) was measured in inversion (INV) and via the anterior drawer test. The weight-bearing lunge test was conducted and DFROM was measured. The data were analyzed using repeated-measures analysis of variance, independent-samples t tests, and 1-way analysis of variance. RESULTS: Of the 55 LASs, 21 (38%) were grade I, 27 (49%) were grade II, and 7 (13%) were grade III. Increases were noted in DFROM over time, between 24 and 72 hours, at 2 to 4 weeks, and at 6 months (P < .05). The DFROM was less in participants with grade III than grade I LASs (P = .004) at 24 to 72 hours; INV length was greater at 24 to 72 hours than at 2 to 4 weeks (P = .023) and at 6 months (P = .035) than at 24 to 72 hours. The anterior drawer length (P = .001) and INV talofibular interval (P = .004) were greater in the LAS group than in the control group at 6 months. CONCLUSIONS: Differences in range of motion and laxity were evident among grades at various time points and may indicate different clinical responses after an LAS.


Subject(s)
Ankle Injuries/physiopathology , Ankle Joint/physiopathology , Athletic Injuries/physiopathology , Joint Instability/physiopathology , Range of Motion, Articular , Sprains and Strains/physiopathology , Ankle Injuries/diagnosis , Cross-Sectional Studies , Female , Humans , Joint Instability/diagnosis , Lateral Ligament, Ankle/injuries , Lateral Ligament, Ankle/physiopathology , Male , Physical Examination , Sprains and Strains/diagnosis , Weight-Bearing , Young Adult
5.
J Environ Qual ; 50(3): 667-679, 2021 May.
Article in English | MEDLINE | ID: mdl-33788277

ABSTRACT

The midwestern United States is a highly productive agricultural region, and extended crop-free periods in winter/spring can result in nitrogen (N) and phosphorus (P) losses to waterways that degrade downstream water quality. Planting winter cover crops can improve soil health while reducing nutrient leaching from farm fields during the fallow period. In this study, we used linear mixed effects models and multivariate statistics to determine the effect of cover crops on soil nutrients by comparing fields with cover crops (n = 9) versus those without (n = 6) in two Indiana agricultural watersheds: the Shatto Ditch Watershed, which had >60% of croppable acres in winter cover crops, and the Kirkpatrick Ditch Watershed, which had ∼20%. We found that cover crops decreased soil nitrate-N by >50% and that the magnitude of reduction was related to the amount of cover crop biomass. In contrast, cover crops had variable effects on water extractable P and Mehlich III soil test P. Finally, cover crop biomass significantly increased soil N mineralization and nitrification rates, demonstrating that cover crops have the potential to supply bioavailable N to cash crop after termination. Our study showed that widespread implementation of winter cover crops holds considerable promise for reducing nutrient loss and improving soil health. The degree to which these results are generalizable across other systems depends on factors such as climate, soil characteristics, and past and current agronomic practices.


Subject(s)
Crops, Agricultural , Soil , Agriculture , Indiana , Midwestern United States , Nitrogen/analysis , Nutrients
6.
Sci Total Environ ; 729: 138744, 2020 Aug 10.
Article in English | MEDLINE | ID: mdl-32498158

ABSTRACT

Floodplain restoration constructed via the two-stage ditch in agricultural streams has the potential to enhance nutrient retention and prevent the eutrophication of downstream ecosystems. Identifying the role of biotic and abiotic factors influencing soluble reactive phosphorus (SRP) retention in floodplains is of interest given that changing redox conditions associated with floodplain inundation can result in a release of geochemically sorbed SRP to the water column. In three agricultural waterways (Indiana, USA), we conducted seasonal measurements of a suite of biogeochemical pools (total P, bioavailable P and Fe) and processes (SRP flux and microbial respiration) from multiple floodplain transects, along with their adjacent stream sediments, to determine the role of biotic and abiotic processes on floodplain SRP retention or release. Across floodplain soils, organic matter explained a significant amount of variation in soil respiration, and SRP flux from the water column to the floodplain soils was driven by the molar ratio of Fe: P, with values >6 indicating potential SRP sorption due to increased available sorption sites. We developed a mass balance model at a single site to relate seasonal floodplain processes with water column SRP export, above and below the study reach, using measurements in this study combined with data from the literature. Grab sample data suggest that the reach retained 26% of incoming SRP, which the mass balance model attributed to seasonal synergy between plant assimilation in spring and summer (removing P from floodplain soils) and abiotic P sorption during winter and spring inundation (adding SRP to the floodplain). Retention of SRP was higher in floodplain soils compared to stream sediments based on the modeled SRP budget. Thus, we suggest that these constructed floodplains will maximize SRP retention from the water column if they inundate regularly, have floodplain soils with Fe:P > 3-6, and that promote sustained plant life.


Subject(s)
Agriculture , Ecosystem , Indiana , Phosphorus , Rivers , Seasons
7.
Eur J Sport Sci ; 20(2): 191-201, 2020 Mar.
Article in English | MEDLINE | ID: mdl-31154941

ABSTRACT

Optimizing joint stiffness through appropriate muscular activation is crucial for maintaining stability and preventing injury. Conditioning techniques may affect joint stability by increasing joint stiffness and altering neuromuscular control; however no studies have assessed this in a controlled setting. Fifteen endurance athletes, 12 power athletes, and 15 control subjects sat on a stiffness device that generated a rapid knee flexion perturbation and were instructed to react to the perturbation. Main outcome measures included short-range (0-4°) and long range (0-40°) stiffness and muscle activation from quadriceps and hamstring muscles. Stiffness results revealed greater short-range stiffness in endurance athletes (0.057 ± 0.012 Nm/deg/kg) than controls (0.047 ± 0.008 Nm/deg/kg, p = 0.021); while passive long-range stiffness was greater in power (0.0020 ± 0.001 nm/deg/kg) than endurance athletes (0.0016 ± 0.001 nm/deg/kg, p = 0.016). Endurance athletes had greater reactive stiffness (0.051 ± 0.017 nm/deg/kg) than control (0.033 ± 0.011 nm/deg/kg, p = 0.001) and power (0.037 ± 0.015 nm/deg/kg, p = 0.044) groups. Endurance athletes also displayed greater quadriceps activity during passive and reactive conditions (p < 0.050) compared to power athletes and controls. These findings suggest that power-based training history may be associated with greater passive joint stiffness across the full range of motion, while endurance-based training could positively influence reactive muscular characteristics, as well as resting muscle tone. These unique variations in stiffness regulation could be beneficial to programmes for prevention and rehabilitation of joint injury.


Subject(s)
Athletes , Hamstring Muscles/physiology , Knee Joint/physiology , Muscle Contraction , Quadriceps Muscle/physiology , Adolescent , Biomechanical Phenomena , Case-Control Studies , Healthy Volunteers , Humans , Male , Young Adult
8.
Environ Manage ; 64(3): 258-271, 2019 Sep.
Article in English | MEDLINE | ID: mdl-31359094

ABSTRACT

The transport and processing of nutrients and organic matter in streams are important functions that influence the condition of watersheds and downstream ecosystems. In this study, we investigated the effects of streambed sediment removal on biogeochemical cycling in Fawn River, a gravel-bottomed river in Indiana, U.S.A. We measured stream metabolism as well as nitrogen (N) and phosphorus (P) retention in both restored and unrestored reaches of Fawn River to examine how sediment removal affected multiple biogeochemical functions at the reach scale. We also assessed the properties of restored and unrestored streambed sediments to elucidate potential mechanisms driving observed reach-scale differences. We found that sediment removal led to lower rates of primary productivity and ecosystem respiration in the restored reach, likely due to macrophyte removal and potentially due to changes to sediment organic matter quality. We found minimal differences in N and P retention, suggesting that these processes are controlled at larger spatial or temporal scales than were examined in this study. Denitrification enzyme activity was lower in sediments from the restored reach compared to the unrestored reach, suggesting that restoration may have decreased N removal. Our results indicate that most near-term changes in biogeochemical function following restoration could be attributed to macrophyte removal, although effects from sediment removal may emerge over longer timescales.


Subject(s)
Ecosystem , Rivers , Geologic Sediments , Indiana , Nitrogen , Phosphorus
9.
BMC Microbiol ; 17(1): 87, 2017 04 04.
Article in English | MEDLINE | ID: mdl-28376715

ABSTRACT

BACKGROUND: The nitrogen (N) cycle consists of complex microbe-mediated transformations driven by a variety of factors, including diversity and concentrations of N compounds. In this study, we examined taxonomic diversity and N substrate utilization by heterotrophic bacteria isolated from streams under complex and simple N-enrichment conditions. RESULTS: Diversity estimates differed among isolates from the enrichments, but no significant composition were detected. Substrate utilization and substrate range of bacterial assemblages differed within and among enrichments types, and not simply between simple and complex N-enrichments. CONCLUSIONS: N substrate use patterns differed between isolates from some complex and simple N-enrichments while others were unexpectedly similar. Taxonomic composition of isolates did not differ among enrichments and was unrelated to N use suggesting strong functional redundancy. Ultimately, our results imply that the available N pool influences physiology and selects for bacteria with various abilities that are unrelated to their taxonomic affiliation.


Subject(s)
Bacteria/isolation & purification , Bacteria/metabolism , Heterotrophic Processes/physiology , Nitrogen/metabolism , Bacteria/classification , Bacteria/genetics , Biodiversity , DNA, Bacterial , Geologic Sediments/microbiology , Nitrogen/chemistry , Nitrogen Cycle , RNA, Ribosomal, 16S/genetics , Rivers/microbiology , Sequence Analysis
10.
Sci Total Environ ; 592: 97-105, 2017 Aug 15.
Article in English | MEDLINE | ID: mdl-28314135

ABSTRACT

The insecticidal Cry1Ab protein expressed by transgenic (Bt) maize can enter adjacent water bodies via multiple pathways, but its fate in stream ecosystems is not as well studied as in terrestrial systems. In this study, we used a combination of field sampling and laboratory experiments to examine the occurrence, leaching, and degradation of soluble Cry1Ab protein derived from Bt maize in agricultural streams. We surveyed 11 agricultural streams in northwestern Indiana, USA, on 6 dates that encompassed the growing season, crop harvest, and snowmelt/spring flooding, and detected Cry1Ab protein in the water column and in flowing subsurface tile drains at concentrations of 3-60ng/L. In a series of laboratory experiments, submerged Bt maize leaves leached Cry1Ab into stream water with 1% of the protein remaining in leaves after 70d. Laboratory experiments suggested that dissolved Cry1Ab protein degraded rapidly in microcosms containing water-column microorganisms, and light did not enhance breakdown by stimulating assimilatory uptake of the protein by autotrophs. The common detection of Cry1Ab protein in streams sampled across an agricultural landscape, combined with laboratory studies showing rapid leaching and degradation, suggests that Cry1Ab may be pseudo-persistent at the watershed scale due to the multiple input pathways from the surrounding terrestrial environment.


Subject(s)
Bacterial Proteins/analysis , Endotoxins/analysis , Hemolysin Proteins/analysis , Plants, Genetically Modified/genetics , Rivers/chemistry , Water Pollutants/analysis , Zea mays/genetics , Bacillus thuringiensis , Bacillus thuringiensis Toxins , Indiana
11.
J Environ Qual ; 44(2): 668-75, 2015 Mar.
Article in English | MEDLINE | ID: mdl-26023984

ABSTRACT

Agricultural activities have heavily altered the nitrogen (N) cycle in stream ecosystems draining croplands, particularly in the midwestern United States. However, our knowledge about dissolved organic N (DON) biogeochemistry in agricultural ecosystems is limited. From January 2011 to June 2012, we investigated DON dynamics in an agricultural headwater stream in the midwestern United States. We quantified the contribution of DON to the total dissolved N (TDN) pool and examined the role of DON as a source of N for microbial metabolism. For this we measured N-acquiring enzyme activities (aminopeptidases) and whole-stream DON uptake through short-term releases of amino acids. To investigate potential coupling between the N and C cycles occurring via simultaneous uptake of these two elements during assimilation of amino acids, seven of the short-term releases were performed concurrently with acetate. We found minimal contribution of DON to the TDN pool in this stream as a result of high concentrations of nitrate. Acetate uptake suggested that C was a limiting factor for microbial metabolism in this stream. In contrast, we were not able to detect amino acid uptake during any of the 13 short-term releases we conducted, likely as a result of high availability of dissolved inorganic N. Aminopeptidase (AMP) activity did not reflect N demand. Large spatial variability in AMP was observed within and among sites, possibly as result of physicochemical characteristics of the sediments. In contrast to other human-dominated streams, DON appeared to play a minor role in microbial metabolic processes and contributed minimally to the N pool of the study stream.

12.
J Athl Train ; 50(7): 726-32, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25811844

ABSTRACT

CONTEXT: The high number of repetitions and high forces associated with overhead throwing lead to anatomical adaptations, such as humeral retrotorsion and posterior-capsule thickness, in elite and professional baseball athletes. However, little is known about the origin and progression of these changes that may account for the increasing trend of chronic shoulder injuries in youth baseball and precipitate subsequent pathologic conditions throughout a young athlete's lifetime. OBJECTIVE: To investigate the relationship of age and upper extremity dominance on humeral retrotorsion, posterior-capsule thickness, and glenohumeral range of motion. DESIGN: Cross-sectional study. SETTING: Research laboratory, local baseball fields, and training facilities. PATIENTS OR OTHER PARTICIPANTS: Thirty-six boys (mean age = 10.94 ± 1.34 years, height = 151.31 ± 12.17 cm, mass = 42.51 ± 10.32 kg) ranging in age from 8 to 12 years and involved in organized youth baseball. MAIN OUTCOME MEASURE(S): Diagnostic ultrasound was used to determine humeral retrotorsion and posterior-capsule thickness. Glenohumeral internal rotation and external rotation were measured using a handheld inclinometer. We used 2 × 2 mixed-model analyses of variance to compare the influence of limb dominance and age on the dependent variables of humeral retrotorsion, posterior-capsule thickness, internal rotation, and external rotation. RESULTS: The dominant shoulders of youth throwers exhibited less glenohumeral internal rotation but greater humeral retrotorsion, posterior-capsule thickness, and glenohumeral external rotation than the nondominant shoulders. Dominant internal rotation was greater in the 8- to 10-year-old group than in the 11- to 12-year-old group, and results trended toward a difference (F1,33 = 4.12, P = .05). Correlations existed between humeral retrotorsion and range of motion (P < .05). CONCLUSIONS: The structural adaptations in the dominant shoulders of younger baseball players were similar to adaptations observed in older baseball athletes, indicating that more examination is needed in younger athletes. We are the first to demonstrate greater posterior-capsule thickness in the dominant shoulders of youth baseball athletes.


Subject(s)
Adaptation, Physiological/physiology , Baseball/physiology , Shoulder Joint/physiology , Analysis of Variance , Athletes , Child , Cross-Sectional Studies , Functional Laterality/physiology , Humans , Male , Range of Motion, Articular/physiology , Rotation , Shoulder Joint/diagnostic imaging , Ultrasonography
13.
J Athl Train ; 50(2): 193-8, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25384002

ABSTRACT

CONTEXT: Lateral ankle sprains are among the most common injuries in sport, with the anterior talofibular ligament (ATFL) most susceptible to damage. Although we understand that after a sprain, scar tissue forms within the ligament, little is known about the morphologic changes in a ligament after injury. OBJECTIVE: To examine whether morphologic differences exist in the thickness of the ATFL in healthy, coper, and unstable-ankle groups. DESIGN: Cross-sectional study. SETTING: Laboratory. PATIENTS OR OTHER PARTICIPANTS: A total of 80 National Collegiate Athletic Association Division I collegiate athletes (43 women, 37 men, age = 18.2 ± 1.1 years, height = 175.8 ± 11.1 cm, body mass = 75.0 ± 16.9 kg) participated in this study. They were categorized into the healthy, coper, or unstable group by history of ankle sprains and score on the Cumberland Ankle Instability Tool. MAIN OUTCOME MEASURE(S): A musculoskeletal sonographic image of the ATFL was obtained from each ankle. Thickness of the ATFL was measured at the midpoint of the ligament between the attachments on the lateral malleolus and talus. RESULTS: A group-by-limb interaction was evident (P = .038). The ATFLs of the injured limb for the coper group (2.20 ± 0.47 mm) and the injured limb for the unstable group (2.28 ± 0.53 mm) were thicker than the ATFL of the "injured" limb of the healthy group (1.95 ± 0.29 mm) at P = .015 and P = .015, respectively. No differences were seen in the uninjured limbs among groups. CONCLUSIONS: Because ATFL thicknesses of the healthy group's uninjured ankles were similar, we contend that lasting morphologic changes occurred in those with a previous injury to the ankle. Similar differences were seen between the injured limbs of the coper and unstable groups, so there must be another explanation for the sensations of instability and the reinjuries in the unstable group.


Subject(s)
Ankle Injuries/complications , Athletic Injuries/diagnosis , Cicatrix , Adolescent , Ankle Joint/diagnostic imaging , Cicatrix/diagnostic imaging , Cicatrix/etiology , Cross-Sectional Studies , Female , Humans , Joint Instability/diagnosis , Joint Instability/etiology , Lateral Ligament, Ankle/injuries , Lateral Ligament, Ankle/pathology , Male , Ultrasonography , United States , Young Adult
14.
Environ Monit Assess ; 187(1): 4208, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25504195

ABSTRACT

Traditional sampling methods for dissolved organic matter (DOM) in streams limit opportunities for long-term studies due to time and cost constraints. Passive DOM samplers were constructed following a design proposed previously which utilizes diethylaminoethyl (DEAE) cellulose as a sampling medium, and they were deployed throughout a temperate stream network in Indiana. Two deployments of the passive samplers were conducted, during which grab samples were frequently collected for comparison. Differences in DOM quality between sites and sampling methods were assessed using several common optical analyses. The analyses revealed significant differences in optical properties between sampling methods, with the passive samplers preferentially collecting terrestrial, humic-like DOM. We assert that the differences in DOM composition from each sampling method were caused by preferential binding of complex humic compounds to the DEAE cellulose in the passive samplers. Nonetheless, the passive samplers may provide a cost-effective, integrated sample of DOM in situations where the bulk DOM pool is composed mainly of terrestrial, humic-like compounds.


Subject(s)
Environmental Monitoring/instrumentation , Humic Substances/analysis , Rivers/chemistry , Water Pollutants/analysis , Cellulose/analysis , Environment , Environmental Monitoring/methods , Indiana
15.
PLoS One ; 9(8): e105149, 2014.
Article in English | MEDLINE | ID: mdl-25171209

ABSTRACT

Denitrifiers remove fixed nitrogen from aquatic environments and hydrologic conditions are one potential driver of denitrification rate and denitrifier community composition. In this study, two agriculturally impacted streams in the Sugar Creek watershed in Indiana, USA with different hydrologic regimes were examined; one stream is seasonally ephemeral because of its source (tile drainage), whereas the other stream has permanent flow. Additionally, a simulated flooding experiment was performed on the riparian benches of the ephemeral stream during a dry period. Denitrification activity was assayed using the chloramphenicol amended acetylene block method and bacterial communities were examined based on quantitative PCR and terminal restriction length polymorphisms of the nitrous oxide reductase (nosZ) and 16S rRNA genes. In the stream channel, hydrology had a substantial impact on denitrification rates, likely by significantly lowering water potential in sediments. Clear patterns in denitrification rates were observed among pre-drying, dry, and post-drying dates; however, a less clear scenario was apparent when analyzing bacterial community structure suggesting that denitrifier community structure and denitrification rate were not strongly coupled. This implies that the nature of the response to short-term hydrologic changes was physiological rather than increases in abundance of denitrifiers or changes in composition of the denitrifier community. Flooding of riparian bench soils had a short-term, transient effect on denitrification rate. Our results imply that brief flooding of riparian zones is unlikely to contribute substantially to removal of nitrate (NO3-) and that seasonal drying of stream channels has a negative impact on NO3- removal, particularly because of the time lag required for denitrification to rebound. This time lag is presumably attributable to the time required for the denitrifiers to respond physiologically rather than a change in abundance or community composition.


Subject(s)
Fresh Water/microbiology , Agriculture , Bacteria/enzymology , Bacteria/genetics , Bacteria/metabolism , Denitrification , Genes, Bacterial , Genes, rRNA , Hydrology , Oxidoreductases/genetics , Polymorphism, Restriction Fragment Length , Seasons
16.
J Mot Behav ; 46(4): 259-65, 2014.
Article in English | MEDLINE | ID: mdl-24731005

ABSTRACT

Effects of muscle fatigue on force coordination and task performance of various manipulation tasks are explored. Grip force (GF; normal force component acting at the digits-object contact area) and load force (LF; tangential component that lifts and holds objects) were recorded prior to and after fatiguing the distal (DAM; i.e., GF producing) and proximal arm muscles (PAM; LF producing). Results reveal a deterioration of GF scaling (i.e., averaged GF-LF ratio), GF-LF coupling (their correlation), and task performance (ability to exert a prescribed LF pattern) associated with DAM, but not PAM fatigue. Deteriorated force coordination clearly increases the likelihood of dropping an object; however, the observed selective effects of DAM and PAM fatigue represent a novel finding deserving of further research.


Subject(s)
Arm/physiology , Muscle Fatigue/physiology , Muscle, Skeletal/physiology , Psychomotor Performance/physiology , Adult , Female , Humans , Male , Young Adult
17.
Hum Mov Sci ; 32(4): 652-67, 2013 Aug.
Article in English | MEDLINE | ID: mdl-24054901

ABSTRACT

Much of the work related to lower extremity inertia manipulations has focused on temporal, kinematic and traditional inverse dynamics assessments during locomotion. Intersegmental dynamics is an analytical technique that provides further insights into mechanisms underlying linked-segment motion. The purpose of this study was to determine how intersegmental dynamics during the swing phase of walking are altered during asymmetrical lower extremity loading. Participants walked overground at a speed of 1.57 ms(-1) with 0, 0.5, 1.0, and 2.0 kg attached to one foot. Net, interaction, gravitational, and muscle moments were computed. Moment magnitudes at joints of the loaded leg increased systematically with increasing load, whereas unloaded leg moments were unaffected by loading. With increasing load, relative contributions of interaction moments about the knee and hip and gravitational moment about the ankle increased (i.e., 21%, 8%, and 44% increases, respectively), whereas the relative contributions of muscle moments about all three joints declined (i.e., -4%, -13%, and -8% decreases for the ankle, knee, and hip, respectively for unloaded vs. 2.0 kg). These results suggest that altered inertia properties of the limb not only affected the amount of muscular effort required to swing the leg, but also changed the nature of the interaction between segments.


Subject(s)
Biomechanical Phenomena/physiology , Functional Laterality/physiology , Muscle, Skeletal/physiology , Walking/physiology , Weight-Bearing/physiology , Adult , Female , Gait/physiology , Gravitation , Humans , Joints/physiology , Male , Models, Theoretical , Muscle Spindles/physiology , Video Recording , Young Adult
18.
Ecol Appl ; 23(3): 583-93, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23734487

ABSTRACT

Pharmaceutical and personal care products are ubiquitous in surface waters but their effects on aquatic biofilms and associated ecosystem properties are not well understood. We measured in situ responses of stream biofilms to six common pharmaceutical compounds (caffeine, cimetidine, ciprofloxacin, diphenhydramine, metformin, ranitidine, and a mixture of each) by deploying pharmaceutical-diffusing substrates in streams in Indiana, Maryland, and New York. Results were consistent across seasons and geographic locations. On average, algal biomass was suppressed by 22%, 4%, 22%, and 18% relative to controls by caffeine, ciprofloxacin, diphenhydramine, and the mixed treatment, respectively. Biofilm respiration was significantly suppressed by caffeine (53%), cimetidine (51%), ciprofloxacin (91%), diphenhydramine (63%), and the mixed treatment (40%). In autumn in New York, photosynthesis was also significantly suppressed by diphenhydramine (99%) and the mixed treatment (88%). Pyrosequencing of 16S rRNA genes was used to examine the effects of caffeine and diphenhydramine on biofilm bacterial community composition at the three sites. Relative to the controls, diphenhydramine exposure significantly altered bacterial community composition and resulted in significant relative increases in Pseudomonas sp. and decreases in Flavobacterium sp. in all three streams. These ubiquitous pharmaceuticals, alone or in combination, influenced stream biofilms, which could have consequences for higher trophic levels and important ecosystem processes.


Subject(s)
Bacteria/drug effects , Biofilms/drug effects , Oxygen Consumption/drug effects , Pharmaceutical Preparations/chemistry , Rivers , Water Pollutants, Chemical/pharmacology , Bacteria/classification , Chlorophyll , Chlorophyll A , Ecosystem , Water Pollutants, Chemical/chemistry
19.
J Orthop Res ; 31(5): 659-64, 2013 May.
Article in English | MEDLINE | ID: mdl-23097326

ABSTRACT

The use of lateral foot wedging in the management of medial knee osteoarthritis is under scrutiny. Interestingly, there have been minimal efforts to evaluate biomechanical effectiveness with long-term use. Therefore, we aimed to evaluate dynamic knee loading (assessed using the knee adduction moment) and other secondary gait parameters in patients with medial knee osteoarthritis wearing lateral foot wedging at a baseline visit and after 1 year of wear. Three-dimensional gait data were captured in an intervention group of 19 patients with symptomatic medial knee osteoarthritis wearing their prescribed laterally wedged foot orthoses at 0 and 12 months. Wedge amounts were prescribed based on symptom response to a step-down test. A control group of 19 patients wearing prescribed neutral orthoses were also captured at 0 and 12 months. The gait of the intervention group wearing neutral orthoses was additionally captured. Walking speed and shoes were controlled. Analyses of variance were conducted to examine for group-by-time (between the groups in their prescribed orthoses) and condition-by-time (within the intervention group) interactions, main effects, and simple effects. We observed increased knee adduction moments and frontal plane motion over time in the control group but not the intervention group. Further, within the intervention group, the mechanical effectiveness of the lateral wedging did not decrease. In patients with medial knee osteoarthritis, the effects of lateral foot wedging on pathomechanics associated with medial knee osteoarthritis were favorable and sustained over time.


Subject(s)
Foot Orthoses , Gait/physiology , Knee Joint/physiology , Osteoarthritis, Knee/therapy , Shoes , Aged , Biomechanical Phenomena , Follow-Up Studies , Humans , Longitudinal Studies , Male , Middle Aged , Osteoarthritis, Knee/physiopathology , Osteoarthritis, Knee/rehabilitation , Walking/physiology
20.
Clin Biomech (Bristol, Avon) ; 28(2): 211-7, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23186619

ABSTRACT

BACKGROUND: Dynamic postural stability is defined as the ability to transition from a dynamic movement to a stable condition over one's base of support. Measures of dynamic stability have been used extensively to classify ankle instability status and assist clinicians with ankle injury interventions. Therefore, the purpose of this study was to determine if current methods of quantifying dynamic stability are accurate in differentiating among healthy, coper, and unstable ankles. METHODS: One hundred ninety four Division-I collegiate athletes (football, volleyball, field hockey, men's/women's soccer, men's/women's lacrosse, men's/women's basketball) volunteered for this study. Participants were categorized into healthy, coper, and stable groups by a self-reported questionnaire and previous history of ankle injuries. Dynamic postural stability was assessed using the Multi-Directional Dynamic Stability Protocol by jumping and landing single-legged onto a force platform from four different directions. Receiver operator curves were used to analyze the accuracy of current techniques of calculating dynamic stability among groups. FINDINGS: None of the existing methods were found to be accurate in differentiating ankle instability status in any of the jump landings. INTERPRETATION: Researchers have commonly used these existing methods to quantify dynamic postural stability. None of the current calculation techniques worked with our jump landing protocol. Researchers need to pay attention to the protocol and calculation technique pairings in that using inaccurate measures of dynamic postural stability makes any findings of that research ineffective. Therefore, this challenges researchers to develop a more accurate calculation to quantify dynamic postural stability, or develop a jump landing protocol that exposes sensorimotor deficits in the more able-bodied population.


Subject(s)
Ankle Joint , Joint Instability/diagnosis , Postural Balance/physiology , Adult , Athletic Injuries/etiology , Athletic Injuries/prevention & control , Female , Humans , Male , ROC Curve , Young Adult
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