ABSTRACT
The ionotropic glutamate receptor subunit GluR6 undergoes developmentally and regionally regulated Q/R site RNA editing that reduces the calcium permeability of GluR6-containing kainate receptors. To investigate the functional significance of this editing in vivo, we engineered mice deficient in GluR6 Q/R site editing. In these mutant mice but not in wild types, NMDA receptor-independent long-term potentiation (LTP) could be induced at the medial perforant path-dentate gyrus synapse. This indicates that kainate receptors with unedited GluR6 subunits can mediate LTP. Behavioral analyses revealed no differences from wild types, but mutant mice were more vulnerable to kainate-induced seizures. Together, these results suggest that GluR6 Q/R site RNA editing may modulate synaptic plasticity and seizure vulnerability.
Subject(s)
Neuronal Plasticity/physiology , RNA Editing/physiology , Receptors, Kainic Acid/metabolism , Seizures/metabolism , Synapses/metabolism , Animals , Binding Sites/genetics , Calcium/metabolism , Cells, Cultured , Dentate Gyrus/cytology , Dentate Gyrus/metabolism , Female , In Vitro Techniques , Kainic Acid , Long-Term Potentiation/physiology , Male , Mice , Mice, Mutant Strains , Neurons/metabolism , Perforant Pathway/cytology , Perforant Pathway/metabolism , Receptors, Kainic Acid/genetics , Seizures/chemically induced , GluK2 Kainate ReceptorABSTRACT
A prospective study was undertaken to measure serum uric acid levels in normal pregnant women of different races, to ascertain if there was any significant interracial variation. A total of 48 women were studied of which 13 were European, 11 New Zealand Maori, 22 Pacific Islanders and 2 Indian. In the second trimester, European, Maori and Cook Island women had similar uric acid levels and other Polynesian groups showed significantly higher levels. In the third trimester, both Maori and Cook Island women showed a marked rise so that their levels came to equal those of other Polynesian groups, all 3 having significantly higher levels than European women. The importance of these observations relates to the use of uric acid levels in the management of patients with gestational proteinuric hypertension. It is possible that in these cases unusually high results may alarm the clinician into hasty intervention.
