Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Photochem Photobiol ; 88(3): 613-9, 2012.
Article in English | MEDLINE | ID: mdl-22128758

ABSTRACT

Hypericin is a natural photosensitizer considered for the new generation of photodynamic therapy (PDT) drugs. The aim of this study was to evaluate the in vitro fungicidal effect of hypericin PDT on various Candida spp., assessing its photocytotoxicity to keratinocytes (HaCaT) and dermal fibroblasts (hNDF) to determine possible side effects. A 3 log fungicidal effect was observed at 0.5 McFarland for two Candida albicans strains, Candida parapsilosis and Candida krusei with hypericin concentrations of 0.625, 1.25, 2.5 and 40 µm, respectively, at a fluence of 18 J cm(-2) (LED lamp emitting at 602 ± 10 nm). To obtain a 6 log reduction, significantly higher hypericin concentrations and light doses were needed (C. albicans 5 µM, C. parapsilosis 320 µM and C. krusei 320 µM; light dose 37 J cm(-2)). Keratinocytes and fibroblasts can be preserved by keeping the hypericin concentration below 1 µm and the light dose below 37 J cm(-2). C. albicans appears to be suitable for treatment with hypericin PDT without significant damage to cutaneous cells.


Subject(s)
Antifungal Agents/pharmacology , Candida/drug effects , Perylene/analogs & derivatives , Photochemotherapy , Photosensitizing Agents/pharmacology , Anthracenes , Candida/classification , Cells, Cultured , Humans , In Vitro Techniques , Microbial Sensitivity Tests , Perylene/pharmacology , Species Specificity
SELECTION OF CITATIONS
SEARCH DETAIL
...