ABSTRACT
Se dosó la actividad de monoamino oxidasa (MAO) plaquetaria en 17 pacientes esquizofrénicos crónicos y en 13 sujetos-control, utilizándose kinuramina como substrato. La actividad de MAO fue aproximadamente 10 veces mayor en los sujetos del grupo control que en los esquizofrénicos (controles: 31,7 + ou - 4,5 nmoles.mg proteína-1.h-1, n=13; esquizofrénicos: 3,3 + ou - 0,6 nmoles.mg proteína-1.h-1, n=17). Los valores de Vmáx y Km para kinuramina fueron significativamente más bajos en os pacientes que en los controles. La actividad de MAO fue aun menor en el subgrupo de pacientes cuya forma clínica previa actual estado residual fue paranoide. Estos resultados permitirían utilizar la determinación de MAO plaquetaria como una variable en la investigación, sobre esquizofrenía (AU)
Subject(s)
Adult , Middle Aged , Humans , Male , Schizophrenia/enzymology , Monoamine Oxidase/metabolism , Blood Platelets/enzymologyABSTRACT
Se dosó la actividad de monoamino oxidasa (MAO) plaquetaria en 17 pacientes esquizofrénicos crónicos y en 13 sujetos-control, utilizándose kinuramina como substrato. La actividad de MAO fue aproximadamente 10 veces mayor en los sujetos del grupo control que en los esquizofrénicos (controles: 31,7 + ou - 4,5 nmoles.mg proteína-1.h-1, n=13; esquizofrénicos: 3,3 + ou - 0,6 nmoles.mg proteína-1.h-1, n=17). Los valores de Vmáx y Km para kinuramina fueron significativamente más bajos en os pacientes que en los controles. La actividad de MAO fue aun menor en el subgrupo de pacientes cuya forma clínica previa actual estado residual fue paranoide. Estos resultados permitirían utilizar la determinación de MAO plaquetaria como una variable en la investigación, sobre esquizofrenía
Subject(s)
Adult , Middle Aged , Humans , Male , Monoamine Oxidase/metabolism , Schizophrenia/enzymology , Blood Platelets/enzymologyABSTRACT
The effect of Pimozide on serum and pituitary concentration of LH was studied in adult male intact rats. Also the release of LH under stimulation of exogenous LH-RH was measured. The treatment for 5 days with pimozide did not alter the serum LH levels, decreased the release of LH by exogenous LH-RH stimulation, and increased the pituitary LH concentration. These results would indicate that blocking the dopaminergic receptors alters pituitary LH release mechanism.
Subject(s)
Gonadotropin-Releasing Hormone/pharmacology , Luteinizing Hormone/metabolism , Pimozide/pharmacology , Animals , Luteinizing Hormone/blood , Male , Pituitary Gland/metabolism , RatsABSTRACT
Seventeen patients (eleven females and six males) with organic hypothalamo-pituitary disease were subjected to a test consisting of a rapid intravenous injection of 50 mug of luteinizing hormone-releasing hormone (LH-RH) at 8:00 A.M., with blood sampling before and 30 and 60 minutes afterward. Two to four weeks later, a rapid intravenous injection of 50 mug of D-Leu-6-des-Gly-10-LH-RH-ethylamide (D-Leu-6-LH-RH-EA) was given under similar conditions, with blood sampling before and 30, 60, and 90 minutes afterward. Serum LH levels were determined by radioimmunoassay. D-Leu-6-LH-RH-EA caused a greater and more sustained rise in serum LH levels than did an equal dose of LH-RH. However, functional classifications of patients were similar with either preparation. This finding suggests that acute administration of D-Leu-6-LH-RH-EA does not cause a higher number of relevant responses as compared with LH-RH, but only a greater stimulation of LH releases in responsive patients.
Subject(s)
Adenoma, Acidophil/diagnosis , Adenoma, Chromophobe/diagnosis , Gonadotropin-Releasing Hormone/analogs & derivatives , Luteinizing Hormone/blood , Pituitary Neoplasms/diagnosis , Adult , Female , Humans , Hypothalamo-Hypophyseal System/physiopathology , MaleABSTRACT
The daily subcutaneous injection of sulpiride produced increase tumor growth and decrease of survival in DBA/2 mice bearing a transplanted mammary gland tumor. It is proposed that sulpiride effect may be mediated by the augment of prolactin level in serum.
Subject(s)
Mammary Neoplasms, Experimental/chemically induced , Sulpiride/adverse effects , Animals , Female , Mice , Mice, Inbred DBA , Neoplasm Transplantation , Sulpiride/administration & dosage , Transplantation, HomologousABSTRACT
The effect of estradiol, actinomycin D, or both, on pituitary response to LH-RH was studied in 6-month-old female rats which had been injected with testosterone propionate on the 2nd day of life. Estradiol significantly augmented the pituitary response to LH-RH. Actinomycin D did not significantly modify basal serum LH levels and pituitary response to LH-RH but did significantly inhibit the augmenting effect of estradiol on such a response.
Subject(s)
Dactinomycin/pharmacology , Estradiol/pharmacology , Gonadotropin-Releasing Hormone/pharmacology , Animals , Drug Synergism , Estrogen Antagonists , Female , Humans , Luteinizing Hormone/metabolism , Ovary/drug effects , Pituitary Gland/drug effects , Pituitary Gland/metabolism , Rats , Testosterone/pharmacologyABSTRACT
The effect of Quinestrol, an estrogenic substance of long-lasting activity, on the pituitary responsiveness to LH-RH was studied in ovariectomized rats. Quinestrol administered as a single 200 pg-dose significantly augmented the pituitary response to LH-RH 36 hr, 9 days, and although not significantly, also at 24 days after its oral administration. No effect could be detected 36 days after its administration. At a 20 pg-dose Quinestrol significantly augmented the pituitary response to LH-RH only at the 36 hr-period studied, this effect not being apparent in later time periods.
Subject(s)
Gonadotropin-Releasing Hormone/pharmacology , Norpregnatrienes/pharmacology , Pituitary Gland/metabolism , Quinestrol/pharmacology , Animals , Castration , Dose-Response Relationship, Drug , Female , Ovary/physiology , Pituitary Gland/drug effects , Quinestrol/administration & dosage , Rats , Stimulation, Chemical , Time FactorsABSTRACT
The effect of the acute or chronic administration of sulpiride on serum prolactin and gonadotropin levels was studied in castrated male rats. Sulpiride administered in acute intravenous injections induced a quick increase in serum prolactin levels and no significant changes in serum gonadotropins. The peak in serum prolactin was observed 30 min after the injection of sulpiride, with a decrease of serum prolactin levels at 60 and 120 min. The subcutaneous administration of sulpiride for 13 days induced a significant increase in serum prolactin levels at the end of the treatment, and no significant changes in serum FSH and LH levels.
Subject(s)
Follicle Stimulating Hormone/blood , Luteinizing Hormone/blood , Prolactin/blood , Animals , Castration , Depression, Chemical , Injections, Intravenous , Injections, Subcutaneous , Male , Rats , Stimulation, Chemical , Sulpiride/administration & dosage , Sulpiride/pharmacology , Testis/physiology , Time FactorsABSTRACT
The pituitary response to LH-RH was studied at 8 a.m., 4 p.m. and 12 p.m. in normal male rats, female rats on day 1 of diestrus and female rats injected with testosterone propionate on the 2nd day of life. Androgenized rats responded to LH-RH with a greater release of LH than did female diestrous and male rats. In the 3 groups the lowest response to LH-RH was observed at 12 p.m., and the highest at 8 a.m., with the exception of androgenized rats which responded with a similar magnitude of LH release at 8 a.m. and 4 p.m. The release of FSH after the injection of LH-RH was studied only at 4 p.m. and only normal male rats responded with a significant FSH release.