ABSTRACT
The detection methods for microbial agents that have eidemiological significance are diversity but cultivation on nutritional media remains the gold standard in microbiological diagnostics. Choice of medium depends on the conditions in which bacteria were early and is present. The nature life determines its physiological peculiarity then a metabolic plasticity promote to survive and to save the virulence. In this review on the example of Yersihia pestis and Vibrio cholerae performed evaluations of the efficient decisions for the bacterial media development. It is declared advantage of baker's yeast hydrolisate as the nutrition media base.
Subject(s)
Culture Media/chemistry , Vibrio cholerae/growth & development , Yersinia pestis/growth & development , HumansABSTRACT
Various protein hydrolysates made in Russia and foreign countries were comparatively evaluated to use them to design a universal agarized culture medium for the diagnosis of plague and cholera. Pancreatic baker's yeast broth was found to be most effective among the test media.
Subject(s)
Cholera/diagnosis , Culture Media/chemistry , Plague/diagnosis , Protein Hydrolysates/chemistry , Vibrio cholerae/isolation & purification , Yersinia pestis/isolation & purification , Humans , Russia , Saccharomyces cerevisiae/chemistry , Vibrio cholerae/growth & development , Yersinia pestis/growth & developmentABSTRACT
A new nutrient medium has been designed to culture and isolate the plague microbe ChDS-37 on the basis of the pancreatic digest of baker's yeast. The results of laboratory tests of the designed medium, by using 10 plague microbe strains and those of approval during the tactical and special training of a specialized antiepidemic team (SAET), suggest that the medium has some advantage over reference media and creates prerequisites for being incorporated into the mobilization reserve of a SAET.
Subject(s)
Culture Media/metabolism , Plague/diagnosis , Yersinia pestis/growth & development , Bacteriological Techniques , Communicable Disease Control , Epidemics/prevention & control , Humans , Plague/microbiology , Practice Guidelines as Topic , Russia , Yersinia pestis/pathogenicityABSTRACT
The influence of amino acids and ammonium salts on the production of cholera enterotoxin (CT) by 3 Vibrio cholerae strains of different biovars and serogroups was evaluated. As revealed in this study, toxin formation in each of the strains was quantitatively and qualitatively determined by their individual sets of amino acids. The amino acid compositions ensuring the maximum production of CT by the V. cholerae strains under study were formed. The use of ammonium salts as the only source of nitrogen in the composition of a synthetic nutrient medium for the accumulation of CT was shown to be inexpedient.
Subject(s)
Bacteriological Techniques , Vibrio cholerae/growth & development , Amino Acids , Culture Media , Quaternary Ammonium CompoundsABSTRACT
Conditions for the cultivation of V. cholerae of different sero- and biovars on tryptone medium, ensuring the maximum production of cholera toxin (CT), dermonecrotic factor (DNF), hemorrhagic factor (HF) and new cholera toxin (NCT) have been determined. The lack of coincidence in the optimum conditions ensuring the maximum production of CT, DNF and HF has been established, which may be indicative of different nature of these toxic substances. NCT, produced by vct- strains, is similar to CN in biological activity as determined in the skin permeability test and in the conditions of accumulation in tryptone medium.
Subject(s)
Vibrio cholerae/pathogenicity , Aerobiosis , Cholera Toxin/biosynthesis , Cholera Toxin/genetics , Cholera Toxin/toxicity , Culture Media , Genome, Bacterial , Methacrylates , Polyurethanes , Temperature , Vibrio cholerae/genetics , Vibrio cholerae/growth & development , Vibrio cholerae/metabolismABSTRACT
Influence of immunomodulators with organometallic origin on sensitivity to mice toxin on CBA, C Bl/6 mice and hybrids F1 (CBA C Bl/6) has been investigated. It has been determined that used immunomodulators are the means of urgent change of sensitivity to plague intoxication (5 min after i.v. administration of immunomodulators, the sensitivity to mice toxin changes). The character of immunomodulators' influence on sensitivity to plague intoxication depends on daily time and animals' genotype.
Subject(s)
Adjuvants, Immunologic/pharmacology , Bacterial Toxins/pharmacology , Organometallic Compounds/pharmacology , Yersinia pestis , Animals , Genotype , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Species SpecificityABSTRACT
Penicillinase produced by a culture of the plague microbe with episomic resistance to benzylpenicillin was isolated and purified. Comparative investigation of the substrate spectrum, Michaelis constant and antigenic properties of the enzymes from the plague and dysentery microbes (R-factor donor) showed that transfer of the R-episome to the new host did not affect the investigated properties of penicillinase the synthesis of which was controlled by this episome.