[Detection of PI-3 viruses, BVD-MD viruses and parvoviruses in bovine fetuses after experimental intrauterine infections]. / Detekce viru PI-3, BVD-MD a parvoviru, persistujicích v bovinních fétech po experimentální intrauterinní infekci.

Growing organ cultures prepared from foetuses experimentally infected in utero by the viruses PI-3 and BVD-MD or bovine parvovirus proved to be suitable in tests with the re-isolation of these viruses 7, 14, 21, 35, 42 and 70 days after infection. The attempts at demonstrating them by the traditional method of the inoculation of the primary cell culture of foetal kidneys were successful only at the re-isolation of the PI-3 virus seven days after infection. The PI-3 virus without cytopathic effect was demonstrated in the primary cell culture prepared from the spleen, lungs, kidneys and testes of foetuses delivered seven days from infection. The cytopathic effect manifested itself after cell transplantation in the first passage when further multiplication of the virus occurred.

[Multiplication of the IBR, PI-3 and BVD-MD viruses in cell and organ cultures of bovine fetuses after experimental intrauterine infections]. / Multiplikace viru IBR, PI-3 a BVD-MD v bunecných a orgánových kulturách pripravených z hovezích fétu po experimentální intrauterinní infekci.

The intrauterine infection of four- to nine-month-old bovine foetuses with the PI-3, BVD-MD viruses, performed 7 to 72 days prior to their delivery, did not exert any significant influence upon the susceptibility of primary cell cultures from foetal organs and tissues to further viral infection in vitro. The BVD-MD and IBR viruses multiplied in the primary cell cultures from the organs of a foetus infected with the PI-3 virus seven days before delivery even in the presence of endogenous PI-3 virus. Persisting infection with the PI-3 virus also failed to influence the susceptibility of foetal organ cultures to infection with the IBR and PI-3 viruses in vitro. The IBR virus and endogenous PI-3 virus multiplied simultaneously to high titres in the organ cultures of thymus and lungs whereas in the organ cultures of kidneys, spleen and testes the multiplication of endogenous PI-3 virus was suppressed.

[Mathematical models in epizootiology]. / Moznosti vyuzití matematických modelu v epizootologii.

Mathematical modelling in epizootology makes it possible to forecast the occurrence and spreading of infection, to learn the main factors of the origin and spread of infection, or to test hypotheses on these factors. Therefore epizootological models must be correct from the biological and mathematical view-point. They should not contradict to experimental facts, must be sufficiently sensitive to important factors, and must be able to approximate real epizootological phenomena and processes. Examples of the construction of simple deterministic and stochastic models of exogenous infections whose etiological agents meet the conditions of Henle-Koch's postulates are used for demonstrating the basic approaches to the use of mathematical models for the evaluation of epizootological analyses and programmes of infection control.