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BACKGROUND: Human babesiosis is a worldwide disease caused by intraerythrocytic protozoa of the genus Babesia. It is transmitted by bites from ixodid ticks, and mechanically transmitted by blood transfusion. It is primarily treated with quinine and/or atovaquone, which are not readily available in China. In this study, we developed a novel treatment regimen involving doxycycline monotherapy in a patient with severe Babesia venatorum infection as an alternative therapeutic medication. The aim of our study is to provide a guidance for clinical practice treatment of human babesiosis. CASE PRESENTATION: A 73-year-old man who had undergone splenectomy and blood transfusion 8 years prior, presented with an unexplained fever, headache, and thrombocytopenia, and was admitted to the Fifth Medical Center of the PLA General Hospital. He was diagnosed with B. venatorum infection by morphological review of thin peripheral blood smears, which was confirmed by multi-gene polymerase chain reaction (PCR), and sequencing of the entire 18s rRNA and partial ß-tubulin encoding genes, as well as isolation by animal inoculation. The doxycycline monotherapy regimen (peros, 0.1 g bisindie) was administered following pharmacological guidance and an effective outcome was observed. The patient recovered rapidly following the doxycycline monotherapy. The protozoan load in peripheral blood samples decreased by 88% in hematocrit counts after 8 days, and negative PCR results were obtained after 90 days of follow-up at the hospital. The treatment lasted for 3 months without any side effects or sequelae. The nine-month follow-up survey of the patient did not reveal any signs of recrudescence or anti-babesial tolerance. CONCLUSIONS: We have reported a clinical case of successful doxycycline monotherapy for human babesiosis caused by B. venatorum, which provides an optional medical intervention for human babesiosis.
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Babesia , Babesiosis , Ixodidae , Male , Animals , Humans , Aged , Babesiosis/drug therapy , Doxycycline/therapeutic use , Ixodidae/parasitology , ChinaABSTRACT
In view of the limitations of the high operational difficulty, safety hazards and adverse reactions of traditional fire needle, and unclear treatment parameters of existing electric fire needles, a new digital electric fire needle instrument was designed and developed in this study. This instrument is a gun type structure, consisting of a gun body, a power supply interface on the gun body, a display unit and a drive unit, a heating unit, a cooling unit, a positioning unit, and a needle inserting unit in the gun body. This instrument can digitally realize the regulation of parameters such as fire needle inserting temperature, depth and speed, and it has the advantageous features of intelligent needle burning, precise positioning, and safe and easy operation. This instrument meets the needs of more patients, medical professionals and scientific researchers, and is conducive to promoting the development of fire needle acupuncture therapy.
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Humans , Needles , Heating , Research Personnel , TemperatureABSTRACT
This study describes the optimization of a eucalyptus elemental chlorine-free (ECF) bleach plant to reduce adsorbable organic halogen (AOX). The correlations between operating conditions of each stage and pulp quality indices as well as the AOX content in wastewater are analyzed, taking an ECF bleaching technology (D0EpPD1) as an example. The calculation models of pulp quality indices and AOX content in wastewater are established. Then, an optimization model aiming at minimizing AOX emission is structured. The model shows a good simulation effect because the errors between the calculated and experimental values are within 6.3%. By analyzing the impact of various operating conditions on AOX emissions, it was found that chlorine dioxide reduced in the D0 stage has the greatest impact on AOX. The optimization results show that AOX can be reduced from 90.84 to 79.58 kg/h, a decrease of 12.5%. The verification experiment results based on the optimized operating conditions showed that the experimental results are in good agreement with the calculated results of the optimization model, and the effect of reducing AOX based on the optimization model is obvious.
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Objective: To analyze the current status of clinical treatment and factors influencing postoperative mortality in infants with critical congenital heart disease (CCHD) in China, optimize the perioperative management of CCHD, and provide a new scientific basis for clinical decision-making for the optimal management of these patients. Methods: This is a retrospective single-center study. Infants diagnosed with CCHD in Guangdong Provincial People's Hospital from January 2017 to December 2019 (aged 0-1 years at admission) were enrolled. General clinical information, inpatient treatment information, prognosis and complications were collected and analyzed. Multivariate logistic regression analysis was used to explore the independent risk factors of postoperative death in infants with CCHD. Results: A total of 826 infants with CCHD were included, including 556 males (67.3%) and the age at first admission was 51.0 (5.0,178.3) days. 264 (32.0%) cases were tetralogy of Fallot and 137 (16.6%) cases were total anomalous pulmonary venous return. 195 cases (23.6%) were diagnosed prenatally. 196 cases (23.7%) were treated with prostaglandin. The preoperative invasive ventilation time was 0 (0, 0) hour, and the postoperative invasive ventilation time was 95.0 (26.0, 151.8) hours. A total of 668 cases (80.9%) underwent surgical treatment. The age was 100.5 (20.0, 218.0) days during operation and the operation time was 190.0 (155.0, 240.0) hours. Sixty-two cases (7.5%) received medical treatment, and 96 cases (11.6%) gave up treatment. A total of 675 cases (81.7%) were discharged with improvement, 96 cases (11.6%) were discharged after giving up treatment, 55 cases (6.7%) died and 109 cases (13.2%) were readmitted within one year. Complications occurred in 565 (68.6%) cases, including pneumonia in 334 cases (40.4%) and cardiac arrhythmias in 182 cases (22.0%). Multifactorial analysis showed that delayed chest closure (OR=49.775, 95%CI 3.291-752.922, P=0.005), prolonged post-operative invasive ventilator ventilation (OR=1.003, 95%CI 1.000-1.005, P=0.038) and cardiac hypoplasia syndrome (OR=272.658, 95%CI 37.861-1 963.589, P<0.001) were the independent risk factors for mortality in CCHD infants post-operation. Conclusions: Tetralogy of Fallot and total anomalous pulmonary venous return account for the majority of infants with CCHD. The proportion of infants diagnosed prenatally was less than 1/4. The majority CCHD infants received surgical treatment. The main complications are pneumonia and arrhythmia. Delayed chest closure, prolonged postoperative invasive ventilator ventilation and low cardiac output syndrome are the independent risk factors for postoperative death in infants with CCHD.
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Humans , Infant , Male , China/epidemiology , Heart Defects, Congenital/therapy , Hospitalization , Prognosis , Retrospective Studies , Risk FactorsABSTRACT
Objective:To explore the molecular targets and therapeutic mechanism of Er Miao San in the treatment of atopic dermatitis (AD), analyzing its active ingredients, moleculartargets and network analysis. Methods:The active ingredients and targets of Er Miao San were obtained from Traditional Chinese Medicine Systems Pharmacology Database (TCMSP) and Drugbank databases. The differential expression genes of AD were obtained by Intergovernmental Group on Earth Observations (GEO) database. The target genes of Er Miao San in the treatment of AD were obtained by using Cytoscape plugin Bisogenet and CytoNCA. Enrichment analysis was obtained from the DAVID database. Results:In total, 12 active ingredients and 107 targets of Er Miao San were screened out by TCMSP and Drugbank databases; 274 differential expression genes (with an adjust P value < 0.005 and |log2 (fold change)|>1.5) were identified between AD patient and control groups using the intergovernmental Group on Earth Observations (GEO) database. 187 target genes of Er Miao San against AD were finally identified by using Cytoscape plugin Bisogenet and CytoNCA. The functional annotations of target genes were related to telomere organization, protein heterotetramerization, regulation of gene expression and so on. Twenty pathways including PI3K-Akt, MAPK and HIF-1 signaling pathway were significantly enriched. Several genes including MAPK1, AKT, RELA and TP53 were the key genes in the gene-pathway network of Er Miao San treating AD. Conclusion:This study suggested that quercetin, tetrahydroberberine, stigmastol and other core ingredientss in Er Miao San may treat AD by participating in PI3K-Akt, MAPK, HIF-1 and other signaling pathways, and regulating the core gene targets of MAPK1, Akt, Rela and TP53.
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Objective:To investigate the potential mechanism by which Brucella suis vaccine strain S2 induces the apoptosis of BV-2 microglia cells, and to discover new protein targets for neurobrucellosis treatment. Methods:BV-2 microglia cells were treated with Brucella suis vaccine strain S2 for 0, 3, 6, 12 and 24 h. Western blot assay and RT-qPCR were performed to detect the expression of p-JNK and p53 at protein and mRNA levels in BV-2 microglia cells. Cell apoptosis was measured by flow cytometry. Immunofluorescence was used to analyze nuclear p-JNK. Results:Brucella suis vaccine strain S2 could promote the expression of p-JNK and p53 at both protein and mRNA levels and increase nuclear p-JNK in BV-2 microglia cells. Moreover, it could also induce the apoptosis of BV-2 microglia cells. Conclusions:Brucella suis vaccine strain S2 could promote the apoptosis of BV-2 microglia cells through activating JNK and promoting p53 expression.
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A multiuser detection (MUD) algorithm based on deep learning network is proposed for the satellite mobile communication system. Due to relative motion between the satellite and users, multiple access interference (MUI) introduced by multipath fading channel reduces system performance. The proposed MUD algorithm based on deep learning network firstly establishes the CINR optimal loss function according to the multiuser access mode and then obtains the best multiuser detection weight through the steepest gradient iteration. Multilayer nonlinear learning obtains interference cancellation sharing weights to achieve maximum signal-to-noise ratio through gradient iteration, which is superior than the traditional serial interference cancellation algorithm and parallel interference cancellation algorithm. Then, the weights with multiuser detection through multilayer network forward learning iteration are obtained with traditional multiuser detecting quality characteristics. The proposed multiuser access detection based on deep learning network algorithm improves the MUD accuracy and reduces the number of traditional multiusers. The performance of the satellite multifading uplink system shows that the proposed deep learning network can provide high precision and better iteration times.
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Algorithms , Computer Communication Networks , Deep Learning , Satellite Communications , Motion , Signal Processing, Computer-Assisted/instrumentation , Signal-To-Noise RatioABSTRACT
@#AIM: To investigate the effect of cardiovascular risk factors on the occurrence of nonarteritic anterior ischemic optic neuropathy(NAION)and visual functions of the patients.<p>METHODS: Sixty-eight patients diagnosed as initial ipsilateral NAION(68 eyes)in NAION group and another 68 patients(68 eyes)matched in age, gender and systemic diseases in Control group were selected from June 2014 to June 2016 were enrolled in this study and evaluated for their levels of homocysteine(Hcy), blood lipids, folic acid and vitamin B12, as well as carotid Doppler ultrasonography. The visual functions were also examined in patients with NAION.<p>RESULTS: The levels of Hcy(24.8±13.9μmol/L), total plasma cholesterol(4.5±1.0mmol/L), triglyceride(2.0±0.9 mmol/L)and low-density lipoprotein(2.9±0.8mmol/L)in NAION patients were significantly higher(<i>P</i><0.05)than those in Control group(11.1±8.2μmol/L, 3.8±0.7mmol/L, 1.5±0.5mmol/L and 2.3±0.5mmol/L)while the level of vitamin B12 decreased significantly(315.6 ±214.5pg/mL, <i>P</i><0.05)in NAION group in comparison with those(467.9±198.2pg/mL)in Control group. However, no significant differences in the artery resistance and inner diameter of the internal carotid were detected between the two groups. The mean deviation(MD)of the visual field was 16.6±7.5dB in NAION group. The levels of Hcy, vitamin B12, folic acid and blood lipid and the presence of systemic diseases were not the risk factors for the visual field damage in NAION patients. MD value was associated with the amplitude and peak latency of P100 waves.<p>CONCLUSION: Hyperhomocysteinemia, hyperlipidemia and low vitamin B12 are the risk factors of in NAION patients. These risk factors, however, are not related to the extent of visual field damage. To some extent, the amplitude and peak latency of visual evoked potentials can reflect the extent of visual field damage.
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BACKGROUND: The effect of extracellular matrix on stem cells is the focus of tissue engineering. However, there are few reports about the synthesis and secretion of extracellular matrix as well as its effects on cells. OBJECTIVE: To isolate, culture and identify rabbit bone marrow mesenchymal stem cells (BMSCs), and to explore the changes of extracellular matrix and whole structure under the intervention of ascorbic acid. METHODS: Rabbit BMSCs were isolated by differential adherent method of the bone marrow, and the expression of CD44, CD45 and CD31 was identified by flow cytometry. The BMSCs were cultured in the culture medium containing 20 mg/L ascorbic acid. Then the cell morphology, gross structure, ultrastructure, and histological changes of BMSCs were observed. The expression of extracellular matrix related genes was detected by RT-PCR. RESULTS AND CONCLUSION: Over 95% passage 2 BMSCs could express CD44, but the expression levels of CD45 and CD31 were extremely low. Intervention with ascorbic acid enhanced the proliferation of BMMSCs with unclear cell boundaries. A cell-sheet structure formed at 10-14 days after intervention. Hematoxylin-eosin staining results showed a layered cell arrangement, and Masson staining findings showed a large amount of extracellular matrix composition. Abundant endoplasmic reticula and vesicle-like structure were observed under the transmission electron microscope. RT-PCR findings showed that ascorbic acid significantly increased the expression of fibronectin mRNA in the BMSCs (P < 0.05), but slightly increased the mRNA expression of collagen type I. All these findings indicate that ascorbic acid not only increases the proliferation and transformation of rabbit BMSCs, but also promotes the synthesis and secretion of extracellular matrix, which has great potential in tissue engineering applications.
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BACKGROUND:Unlike linear RNAs,circular RNAs (circRNAs) are a novel type of RNA which can form covalently closed circles and are highly expressed in eukaryotic transcriptomes.In the plenitude of naturally occurring RNAs,circRNAs and their biological role are underestimated for years.Recent studies have discovered thousands of endogenous circRNAs in mammalian cells.OBJECTIVE:To review the formation,properties,and functions of circRNAs,and their potential significance in diseases.METHODS:A computer-based search for literature in CNKI and PubMed databases published from January 2000 to December 2016 was performed using the keywords of "circRNA,miRNA,function,mechanism" in English and Chinese,respectively.Finally,62 eligible articles were included for analysis.RESULTS AND CONCLUSION:CircRNAs are largely generated from exonic or intronic sequences,and reverse complementary sequences or RNA-binding proteins are necessary for circRNA biogenesis.A majority of circRNAs that are conserved across specie are stable and resistant to RNaseR,and often exhibit tissue/developmental-stage-specific expression.Recent research has revealed that circRNAs can function as microRNA sponges,regulators of splicing and transcription,and modifiers of parental gene expression.Emerging evidence indicates that circRNAs might play important roles in atherosclerotic vascular diseases,neurological disorders,prion diseases and cancer;exhibit aberrant expression in colorectal cancer;and serve as diagnostic or predictive biomarkers of some diseases.Similar to microRNAs and long noncoding RNAs,circRNAs are arousing general interest in the field of RNA and widely participate in the life process.
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Objective To investigate the effect of X-ray irradiation on neurites growth of primary hippocampal neurons in vitro.Methods Primary hippocampal neuron culture in vitro were irradiated with 0,2,4,8,10,12 Gy of X-rays.In the first day and the third day after irradiation,the cell death of primary hippocampal neurons was detected by MTT method,and the morphological changes of primary hippocampal neuronal neurites were detected with immunofluorescence staining method.Results In the first day and third day after irradiation,the cell death of hippocampal neurons increased significantly (F =123.068,43.370,P ＜0.05),but there were no significant difference among 4,8,10,and 12 Gy irradiation groups.Immunofluorescence staining showed that,in the first day after irradiation,the neurite length and total dendritic branch length (TDBL) were significantly changed (F =9.169,7.856,P ＜0.05),and in the third day after radiation,the neurite length,TDBL and total dendritic branch tip number (TDBTN) were also altered (F =23.797,6.565,6.021,P ＜ 0.05).Conclusion X-ray irradiation can inhibit the growth of neurites in the primary hippocampal neurons in vitro.
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<p><b>BACKGROUND</b>Insulin-like growth factor-binding protein-3 (IGFBP-3) is suggested to predict the radiosensitivity and/or prognosis of patients with esophageal squamous cell carcinoma (ESCC). The present study was designed to investigate the clinical and prognostic effects of IGFBP-3 on ESCC.</p><p><b>METHODS</b>IGFBP-3 was detected by immunohistochemistry in paraffin-embedded tissues from 70 ESCC patients treated with radiotherapy alone and further examined by western blotting analysis in 10 pairs of fresh ESCC tissues and adjacent non-malignant esophageal specimens. Receiver operating characteristic (ROC) analysis was used to determine cut-off scores for tumor positivity and to evaluate patient survival status. The χ(2) test was performed to analyze the association of IGFBP-3 expression with clinical characteristics and radiotherapy response. Associations between prognostic outcomes and IGFBP-3 expression were investigated using Kaplan-Meier analysis and the Cox proportional hazards model.</p><p><b>RESULTS</b>The threshold for IGFBP-3 positivity was set to greater than 65% [area under the ROC curve (AUC)=0.690, P<0.019]. Of the 70 ESCC patient tissues tested, 32 (45.7%) were defined as having high IGFBP-3 expression. The levels of IGFBP-3 protein expression were decreased in 70.0% (7 of 10) of ESCC tissues compared with adjacent non-malignant esophageal tissue. In addition, IGFBP-3 expression was associated with pathologic classification (P<0.05 for T, N, and M categories and clinical stage). Patients with elevated protein level of IGFBP-3 in the tumor had an improved radiotherapy response and prolonged overall survival (P<0.001).</p><p><b>CONCLUSIONS</b>High level of IGFBP-3 expression in ESCC associates with early clinical stages and are predictive for favorable survival of the patients treated with radiotherapy.</p>
Subject(s)
Humans , Blotting, Western , Carcinoma, Squamous Cell , Esophageal Neoplasms , Immunohistochemistry , Insulin-Like Growth Factor Binding Protein 3 , Kaplan-Meier Estimate , Prognosis , Proportional Hazards Models , ROC Curve , Radiation-Sensitizing AgentsABSTRACT
Objective To discuss the differences of the risk factors between Bell Stage Ⅰ and Stage Ⅱ/Ⅲ of neonatal necrotizing enterocolitis (NEC),in order to provide theoretical basis for better preventing and treating NEC of each stages.Methods Eighty seven NEC neonates'references from 2009 to 2011 were all analyzed retrospectively to find out relative risk factors.They were divided into 2 groups according to Bell stage:stage Ⅰ and stage Ⅱ/Ⅲ.Twenty four items were compared between the 2 groups,including perinatal factors,feeding methods,complications,RBC perfusion,etc,using SPSS 17.0 software to make single factor analysis,for the sake of finding out relative risk factors.Results The comparison of gestational age,birth weight,onset age,and onset weight between 2 groups has no statistical significance (P ＞ 0.05).The mortality of stage Ⅱ / Ⅲ was higher than stage Ⅰ (P ＜ 0.05).Seven of 24 items have statistical difference in the comparison (P ＜ 0.05),including:Gestational diabetes mellitus,amniotic fluid disorders,respiratory failure,hypothyroid,anemia,sepsis,RBC perfusion,indicating that these items may correlate to incidence and severity of NEC.Conclusions Gestational diabetes mellitus,amniotic fluid disorders,respiratory failure,hypothyroid,anemia,sepsis,RBC perfusion are risk factors that influence the severity of NEC.
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Objective To improve the quality of transportation of critically ill children in long distance interhospital transport.Methods The clinical data,diagnosis and outcomes of 507 critically ill children transported from other hospitals in long distances to PICU of Guangdong General Hospital,between Aug.2008 and Jul.2011,were analyzed retrospectively.Results Among 507 critically ill children,336 cases were male and 171 cases were female,aged from 29 days to 15 years(median age was 11 months) and weighted from 2.5 to 80.0 kg(median weight was 8.0 kg).The transport durations ranged from 90 to 990 min (median duration was 264 min),among which 121 cases(23.9％)took 121 to 240 min,288 cases (56.8％) took 241 to 480 min,72 cases(14.2％) took more than 480 min.The transfer distance ranged from 74 to 620 km (median distance was 266 km),249 cases(49.1％) of which were transferred from hospitals ranging from 101 to 200 km,133 cases(26.2％) less than 100 km.The top 3 diseases were cardiovascular diseases(169 patients,33.3％),respiratory diseases (128 patients,25.2％) and neural diseases (85 patients,16.8％).None of the patients died on the way and all of them received further treatment.In the end,486 cases discharged,13 cases gave up and 8 cases died.Conclusions During the long distance interhospital transport,a specialist retrieval team with adequate equipments and preparation,close monitoring on the way,can effectively improve the quality of long distance interhospital transportation.
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Objective To investigate the incidence and short-term outcome of very preterm infants with physiologic bronchopulmonary dysplasia (BPD).Methods Data of very preterm infants with gestational age of no more than 32 weeks admitted into Guangdong General Hospital between Mar.2011 and Feb.2012 were prospectively collected.Oxygen withdrawing test was performed in these infants at postmenstrual age of 36 weeks.Infants who failed to pass the test or who were ventilated were diagnosed as physiologic BPD.Results Sixty-six infants were admitted,of whom 6 were excluded for more than 36 weeks of postmenstrual age at admission.Eighteen infants(30.0％) were diagnosed as classic BPD.Among them,at testing time point,4 cases did not need supplemental oxygen,13 cases needed oxygen sup-plementation and 1 case needed mechanical ventilation.Thirteen infants underwent oxygen withdrawing test and 6 cases passed.Eight cases (13.3％) were diagnosed as physiologic BPD.The incidences of apnea or bradycardia were of no differences between infants passing or failing to pass oxygen withdrawing test.All infants survived to discharge without supplemental oxygen.Conclusions The incidence of infants with physiologic BPD is significantly lower than that with classic BPD.Restrictive saturation of percutaneous oxygen can decrease the incidence of infants with supplemental oxygen,with no more adverse events.More research are needed on physiologic BPD.
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<p><b>OBJECTIVE</b>To assess agreement between the ultrasonic cardiac output monitor (USCOM) and conventional echocardiography (ECHO) in the measurement of cardiac output in newborn infants, investigate the accuracy and clinical utility of the USCOM in healthy neonates. To explore a more convenient, faster, more accurate hemodynamic monitoring method, for improving the outcome of the critically ill neonates.</p><p><b>METHOD</b>From October 1(st), 2011 to March 31(st), 2012, a total of 49 infants were included, 20 were term infants, 29 were preterm infants. Cardiac outputs were measured by both ultrasonic cardiac output monitor and echocardiography in all the infants, 60 times measurements were done in both the term infants the preterm infants. The cardiac output of the left and right ventricles, heart rate, diameter and velocity time integral of the aortic valve and pulmonary artery valve of each infant were recorded. The consistency of two methods was analyzed as described by Bland-Altman.</p><p><b>RESULT</b>Term the term infant group includea 20 term infants, 11 were male and 9 were female, the mean gestational age were (38.1 ± 0.56) weeks, mean age were (2 ± 1) days, mean weight were (3.2 ± 0.29) kg, mean Apgar score were 10. The mean left ventricular output measured by Echo was (242.3 ± 38.9) ml/(kg·min), measured by USCOM was (211.7 ± 38.5) ml/(kg·min); The mean right ventricular output measured by ECHO was (318.9 ± 47.0) ml/(kg·min), measured by USCOM was (340.7 ± 76) ml/(kg·min). Agreement between Echo and USCOM for left ventricular output (LVO) was (bias, ± limits of agreement, mean % error): (30.6 ± 51.1) ml/(kg·min), 21%, and for right ventricular output (RVO): (-21.8 ± 105) ml/(kg·min), 33.2%. The diameter of the aortic valve and pulmonary artery valve measured by conventional echocardiography were significantly larger than that estimated by ultrasonic cardiac output monitor (P < 0.001). The velocity time integral of the pulmonary artery valve measured by ultrasonic cardiac output monitor were significantly larger than measured by conventional echocardiography (P < 0.001). The heart rate, velocity time integral of the aortic valve measured by two methods had no significant differences (P > 0.05). The preterm neonates group included 29 preterm infants, 18 were male and 11 were female, the mean gestational age were (32.6 ± 2.8) weeks, mean age were (2 ± 1) days, mean weight were (1.88 ± 0.57) kg. All the infants were diagnosis as preterm infant, low birth weight. The mean left ventricular output measured by ECHO was (259.8 ± 70) ml/(kg·min), measured by USCOM was (235.6 ± 61.8) ml/(kg·min), the mean right ventricular output measured by ECHO was (318.9 ± 47.0) ml/(kg·min), measured by USCOM was (340.7 ± 76) ml/(kg·min). Agreement between Echo and USCOM for left ventricular output (LVO) was (bias, ± limits of agreement, mean % error): (24.1 ± 71.2) ml/(kg·min), 27.4%, and for right ventricular output (RVO): (-29.5 ± 192.9) ml/(kg·min), 51.8%. The diameter of the aortic valve and pulmonary artery valve measured by conventional echocardiography were significantly larger than estimated by ultrasonic cardiac output monitor (P < 0.001). The velocity time integral of the pulmonary artery valve measured by USCOM were significantly larger than that measured by conventional echocardiography (P < 0.001). The heart rate, velocity time integral of the aortic valve measured by two methods had no significant differences (P > 0.05).</p><p><b>CONCLUSION</b>Agreement between USCOM and conventional ECHO in the LVO measurement is acceptable, both in the term group and the preterm group. LVO measurement measured by USCOM is recommended. The accuracy and clinical utility of the USCOM in neonates is acceptable. USCOM is a convenient, fast and accurate hemodynamic monitoring method in neonates. While the agreement between USCOM and conventional ECHO in the RVO measurement is poor, especially in the preterm group, the results of the RVO cannot be considered interchangeable in the two methods.</p>
Subject(s)
Female , Humans , Infant , Infant, Newborn , Male , Cardiac Output , Echocardiography, Doppler , Methods , Heart Rate , Physiology , Hemodynamics , Physiology , Infant, Premature , Intensive Care, Neonatal , Monitoring, Physiologic , Methods , Reproducibility of Results , Sensitivity and Specificity , Ventricular Function , PhysiologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of iron supplement on function of mitochondrial respiratory of liver during exercise-induced hypochromic rats.</p><p><b>METHOD</b>Forty healthy male Wistar rats were randomized into 5 groups (n = 8): static control (C), exercise-training (T), training with supplementation of small dose iron (S + T), training with supplementation of middle dose iron (M + T) and training with supplementation of large dose iron (L + T). Training performed incremental exercise for 8 weeks, 6 days/week, iron supplementation from the fifth week. Liver were prepared immediately after exhaustive running. Liver mitochondria were extracted by differential centrifugation. Spectrophotometric analysis was used to evaluate activities of electron transport chain complex (C) I-IV in liver mitochondria.</p><p><b>RESULTS</b>(1) C I, CII and CIV activities in T group were increased significantly (P < 0.05, P < 0.01), CI - C IV activities in S + T, M + T and L + T groups were increased significantly (P < 0.05, P < 0.01) compared with those in C group. (2) CII activity in S + T group was increased remarkably (P < 0.05); CIII and CIV activities in M + T group were increased remarkably (P < 0.01); CI - CIV activities in L+ T group were increased remarkably (P < 0.05, P < 0.01) compared with those in T group.</p><p><b>CONCLUSION</b>Large load exercise training composite iron supplementation can improve function of mitochondrial respiration of liver and the aerobic capacity. From the athletic ability , the middle dose iron supplementation is better during large load exercise training.</p>
Subject(s)
Animals , Male , Rats , Anemia, Hypochromic , Metabolism , Cell Respiration , Hemoglobins , Metabolism , Iron , Pharmacology , Mitochondria, Liver , Physiology , Physical Conditioning, Animal , Rats, WistarABSTRACT
Objective To investigate the effect of different mechanical environment ( in vivo and in vitro) on expression of basic fibroblast growth factor (bFGF) and explore the role of mechanical stimulation in corneal tissue repair after laser assisted in situ keratomileusis (LASIK) surgery. Methods Animal models by LASIK surgery were established to keep the corneas under different mechanical environment. The experimental animals were killed at the first week or the first month after LASIK surgery to obtain the corneas. In addition, the primary corneal fibroblasts were subjected to cyclic mechanical stretch (0.1 Hz; 5%, 10%, 15% stretch; 6 h or 24 h) using Flexcell 4 000 tension system. Expression of bFGF was determined by ELISA method. Results At the first week after LASIK surgery, expression of bFGF was increased significantly in 30% group (residual stroma bed accounting for 30% of the whole cornea), as compared with the control group (P<0.05), and then it was decreased to the normal level in all groups at the first month after LASIK surgery. Analysis on the same surgery method at different time showed that there were significant differences only in 30% group at the first week and month (P<0.05). Cyclic stretch experiment in vitro indicated that bFGF expression in 15% stretch group was significantly increased after 6 h than that in the control group (P<0.05), with a significant decrease after 24 h (P<0.05). Conclusions Mechanical stimulation can regulate bFGF expression of corneal tissues and corneal frbroblasts, and bFGF plays a positive role in the early corneal tissue repair after LASIK surgery.
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Objective To discuss the appropriate timing of providing enteral nutrition through nasojejunal tube for patients with severe craniocerebral injury.Methods 126 cases of patients were divided into 3 groups randomly,providing enteral nutrition through naso-jejunal tube for the first group,the second group and the third group within 12～24 hours,48 hours later and 72 hours later after injury respectively.The nutrition situation of 3 groups was recorded 6 hours later,48 hours later,on the 5th day and the 10th day,including indicators such as total serum protein,blood albumin,serum creatinine,etc and complication cases of diarrhea,hemorrhage of digestive tract,palirrhea,aspiration,inhalation pneumonia and so on within 2 weeks after injury.Results In terms of indicators of albumin,creatinine 48 hours after injury and total protein,albumin and creatinine on the 5th day and 10th day,the first group were better than the second and third group,there were statistic differences between the three groups.Complication comparison within 2 weeks after injury:the difference of palirrhea cases among the three groups was significant,the third group had a higher ratio than the first and second group.And there was no statistic difference in the other indicators like diarrhea,hemorrhage of digestive tract,aspiration and inhalation pneumonia.Conclusions It is high time that patients with simple severe craniocerebral injury are provided with enteral nutrition through naso-jejunal tube within 12 to 24 hours,which can improve patients nutrition situation without the increase of the complications.
