ABSTRACT
OBJECTIVE: To establish chromatographic fingerprint of components of Menoprogen absorbed into blood by RP-HPLC. METHODS: Kromasil C18 column was used, with of 0.1% phosphoric acid and acetonitrile as mobile phase in a gradient elution. The flow rate was 1.0 mL/min, the column temperature was 30 degrees C, the detection wavelength was 205 nm. 10 SD rats were administered 10 different batches of Menoprogen respectively. RESULTS: The fingerprint of Menoprogen was established. 18 common peaks were identified, the similarities were over 0. 95. By comparison drug and blank serum wiith contained drug serum, identified eight prototype components aborbed into blood directly and 10 metabolic components. CONCLUSION: The serum fingerprint of Menoprogen is established for the first time. 18 components are identifided in blood,one of them is hyperoside. It can reveal the change of chemical constituents after ingestion, and provide some data on material basis study in vivo for Menoprogen.
