Funcionamiento afectivo-motivacional de pacientes prediabéticos y diabéticos / Affective-motivational functioning in prediabetic and diabetic patients

Fundamento: El funcionamiento afectivo-motivacional influye en el control de las enfermedades crónicas y en la actitud que asumen los enfermos ante la enfermedad. Objetivo: Caracterizar las particularidades afectivo-motivacionales que diferencian la regulación del comportamiento en pacientes prediabéticos y diabéticos crónicos. Metodología: Se utilizó un paradigma mixto de investigación, con diseño exploratorio secuencial. La muestra quedó conformada por un total de 20 sujetos, distribuidos en dos grupos: uno integrado por pacientes prediabéticos y el otro por pacientes diabéticos. Se aplicó el RAMDI (S), la escala Dembo - Rubinstein, el test de completamiento de frases, la técnica de la composición. Resultados: Se revelaron diferencias afectivo-motivacionales entre ambos grupos, expresadas en la concientización o no de la enfermedad y la consecuente regulación del comportamiento. Predominó una tendencia a la sobrevaloración en la variable salud, en los pacientes prediabéticos y subvaloración en los pacientes diabéticos. Conclusiones: Los sujetos prediabéticos no se reconocen como enfermos, no valoran los riesgos que contraen con su condición y no se comprometen con un cambio de estilo de vida. El grupo de pacientes diabéticos se caracterizó por expresar conciencia de la enfermedad, compromiso afectivo y elaboración personal, como procesos que facilitan la regulación de su comportamiento.

Background: Affective-motivational functioning affects the control over chronic diseases and the attitudes that patients assume towards the disease. Objective: To characterize the affective-motivational specificities that distinguish behavioral adaptations in prediabetic and chronic diabetic patients. Methods: A mixed research paradigm was applied, with a sequential exploratory design. The sample was composed by a total number of 20 patients, distributed in two groups: one composed by prediabetic patients and the other by diabetic ones. The RAMDI (S), the Dembo-Rubinstein Scale, and the Sentence Completion Test were applied, the Composition Technique. Results: Affective-motivational differences between the two groups were detected, manifested in the consciousness or not of the disease and consequent behavior regulation. A tendency to overestimate the health variable prediabetic patients and underestimate diabetic patients prevailed. Conclusions: Prediabetic patients do not recognize themselves as sick, do not value the risks they take with their status and do not engage in a lifestyle change. The diabetic patient group was characterized by expressing disease self-consciousness, affective compromise and personal development, as the processes that facilitate the adjustment of their behavior.

Características clínicas y microbiológicas de la infección de vías urinarias bajas en población ambulatoria / Clinical and Microbiological Characteristics of Lower Urinary Tract Infections in an Outpatient Population

Introducción: El tratamiento de las infecciones del tracto urinario es casi siempre empírico, lo que genera una serie de problemas en la consulta diaria. Objetivo: Caracterizar clínica y microbiológicamente las infecciones de vías urinarias bajas no complicadas en pacientes de una clínica de primer nivel. Métodos: Se realizó un estudio transversal descriptivo. La identificación de las bacterias del cultivo de orina se efectuó por métodos establecidos. La prueba de susceptibilidad a los antimicrobianos se realizó por la técnica Kirby-Bauer. Se utilizó el programa estadístico SPSS versión 26, con la prueba de ji al cuadrado y un análisis multivariado discriminante. Se calculó también razón de momios con el programa Epi-Info. Resultados: Se incluyeron 270 pacientes, con frecuencia de 39,3 por ciento de cultivos positivos, y Escherichia coli como la especie predominante. Se identificaron, además, 31,3 por ciento de bacterias Gram positivas. Se presentó significancia estadística entre la infección urinaria y factores como el sexo, y la infección del tracto urinario previa en las mujeres. Se obtuvo 100 por ciento de cepas resistentes a ampicilina. En general, se obtuvieron porcentajes de resistencia altos en los antimicrobianos probados. Conclusiones: Escherichia coli fue la especie más frecuentemente aislada, sin embargo, existe una serie de microorganismos implicados en enfermedades del tracto genital como Gardnerella vaginalis, que parecen estar involucrados en la etiología de las infecciones del tracto urinario. Se identificaron factores de riesgo como el sexo biológico y las infecciones previas en mujeres. Se obtuvieron porcentajes de resistencia altos en los antimicrobianos probados(AU)

Introduction: The management of urinary tract infections is almost always empirical, which generates a series of problems in the daily consultation. Objective: To characterize, clinically and microbiologically, uncomplicated lower urinary tract infections in patients of a primary level clinic. Methods: A descriptive and cross-sectional study was carried out. Bacterial identification in urine culture was performed by established methods. Antimicrobial susceptibility testing was performed using the Kirby-Bauer technique. The statistical software SPSS (version 26) was used, with the chi squared test and multivariate discriminant analysis. Odds ratios were also calculated with the Epi-Info program. Results: A total of 270 patients were included, with a 39.3percent frequency of positive cultures and Escherichia coli as the predominant species. In addition, 31.3percent of Gram-positive bacteria were identified. There was statistical significance between urinary tract infection and factors such as sex or previous urinary tract infection in women. One result was 100percent of ampicillin-resistant strains. In general, high percentages of resistance were obtained for the tested antimicrobials. Conclusions: Escherichia coli was the most frequently isolated species; however, there is a number of microorganisms implicated in genital tract diseases, such as Gardnerella vaginalis, which appear to be involved in the etiology of urinary tract infections. Risk factors such as biological sex and previous infections in women were identified. High percentages of resistance were obtained for the tested antimicrobials(AU)

Particularidades del funcionamiento afectivo-motivacional de pacientes prediabéticos / Particularities of the motivational and affective functioning in prediabetic patients

Introducción: La prevención en el progreso de la prediabetes hacia la diabetes, debe hacerse desde un enfoque holístico y considerar el funcionamiento afectivo - motivacional de quienes la padecen. Objetivo: Determinar las particularidades del funcionamiento afectivo - motivacional de pacientes prediabéticos. Métodos: Se desarrolló un estudio exploratorio descriptivo con metodología mixta, se integraron los análisis cuantitativo y cualitativo; la muestra quedó conformada por 10 sujetos prediabéticos. Para la pesquisa se realizó el análisis psicológico de los pacientes, según su historia clínica y se aplicaron el Registro de la Actividad. Método Directo e Indirecto (RAMDI), la Escala Dembo-Rubinstein, las Técnicas de Completamiento de Frases y de Composición, así como la entrevista semiestructurada. Resultados: Los sujetos prediabéticos no se reconocen como enfermos, no valoran los riesgos que contraen por esa condición y no se comprometen con un cambio de estilo de vida. Conclusiones: En los pacientes prediabéticos la categoría salud no es un motivo rector que oriente y regule su conducta de modo estable; predominó la actitud de «no aceptación de la enfermedad», con una marcada tendencia a la autovaloración inadecuada que limita la responsabilidad personal ante la condición mórbida.

Introduction: the prevention of the progression from prediabetes to diabetes must be done from a holistic approach considering the motivational and affective functioning of the patients who suffer from this condition. Objective: to determine the particularities of the motivational and affective functioning of prediabetic patients. Methods: a descriptive exploratory study was carried out with a mixed-method research where quantitative and qualitative analyzes were integrated; the sample was made up of 10 prediabetic subjects. A psychological assessment was made based on their clinical history and the Register of the Activity. Direct and Indirect Method (RAMDI in Spanish), the Dembo-Rubinstein Scale, the Sentence Completion and the Composition Techniques, as well as the semi-structured interview were applied for screening. Results: the prediabetic subjects do not recognize themselves as sick, do not value the risks they incur with their condition, and do not commit to a lifestyle change. Conclusions: health category in prediabetic patients is not a guiding motive that guides and regulates their behaviour in a stable way; a predominance of an attitude of "non-acceptance of the disease", with a marked tendency to inadequate self-assessment that limited personal responsibility for the morbid condition.

Role of the MicroRNAs in the Pathogenic Mechanism of Painful Symptoms in Long COVID: Systematic Review.

The ongoing pandemic of COVID-19 has caused more than 6.7 million tragic deaths, plus, a large percentage of people who survived it present a myriad of chronic symptoms that last for at least 6 months; this has been named as long COVID. Some of the most prevalent are painful symptoms like headache, joint pain, migraine, neuropathic-like pain, fatigue and myalgia. MicroRNAs are small non-coding RNAs that regulate genes, and their involvement in several pathologies has been extensively shown. A deregulation of miRNAs has been observed in patients with COVID-19. The objective of the present systematic review was to show the prevalence of chronic pain-like symptoms of patients with long COVID and based on the expression of miRNAs in patients with COVID-19, and to present a proposal on how they may be involved in the pathogenic mechanisms of chronic pain-like symptoms. A systematic review was carried out in online databases for original articles published between March 2020 to April 2022; the systematic review followed the PRISMA guidelines, and it was registered in PROSPERO with registration number CRD42022318992. A total of 22 articles were included for the evaluation of miRNAs and 20 regarding long COVID; the overall prevalence of pain-like symptoms was around 10 to 87%, plus, the miRNAs that were commonly up and downregulated were miR-21-5p, miR-29a,b,c-3p miR-92a,b-3p, miR-92b-5p, miR-126-3p, miR-150-5p, miR-155-5p, miR-200a, c-3p, miR-320a,b,c,d,e-3p, and miR-451a. The molecular pathways that we hypothesized to be modulated by these miRNAs are the IL-6/STAT3 proinflammatory axis and the compromise of the blood-nerve barrier; these two mechanisms could be associated with the prevalence of fatigue and chronic pain in the long COVID population, plus they could be novel pharmacological targets in order to reduce and prevent these symptoms.

Particularidades de la esfera afectivo motivacional en pacientes diabéticos crónicos

Introducción : La diabetes mellitus, debe abordarse desde un enfoque holístico, donde se considere el funcionamiento afectivo motivacional de los pacientes que la padecen. Objetivo: Determinar las particularidades afectivo motivacionales en pacientes diabéticos crónicos. Métodos: Se realizó un estudio exploratorio descriptivo con un paradigma de investigación mixto, donde se integraron los análisis cuantitativo y cualitativo. La muestra quedó conformada por 10 sujetos diabéticos crónicos, no se pretendió que los hallazgos derivados de su estudio fuesen generalizados a la población, pero sí, por su carácter inductivo, reflejaran la perspectiva de aquel que vivencia el fenómeno objeto de estudio. Para la pesquisa se aplicó el análisis psicológico de la historia clínica, el RAMDI (S), la Escala Dembo -Rubinstein, el test de completamiento de frases, la técnica de la composición, así como la entrevista semiestructurada. Resultados: Se constató conciencia de la enfermedad, se manifestó una adecuada valoración de la misma, acompañado de un compromiso afectivo y una elaboración personal en sus reflexiones, donde se conjugan sus vivencias y experiencias de vida, que da una orientación afectivo-motivacional a su comportamiento. Conclusiones: La categoría salud aparece como un motivo central y jerárquico en estos sujetos, pero a pesar de esto no todos logran regular su comportamiento de una manera efectiva y saludable.

Introduction: Diabetes mellitus should be approached from a holistic approach, where the motivational affective functioning of patients suffering from it is considered. Objective: To determine the motivational affective characteristics in chronic diabetic patients. Methods: A descriptive exploratory study was carried out with a mixed research paradigm, where quantitative and qualitative analyzes were integrated. Responding to this approach, the sample was made up of 10 chronic diabetic subjects, not intending that the findings derived from their study were generalized to the population, but yes, due to their inductive nature, they reflected the perspective of the person experiencing the phenomenon under study. For the research, the psychological analysis of the clinical history, the RAMDI (S), the Dembo-Rubinstein Scale, the Phrase Completion Test, the Composition Technique, as well as the semi-structured interview were applied. Results: Awareness of the disease was found, showing an adequate assessment of it, accompanied by an emotional commitment and personal elaboration in their reflections, where their experiences and life experiences are combined, and giving an emotional-motivational orientation to their behavior. Conclusions: The health category appears as a central and hierarchical motive in these subjects, but despite this, not everyone manages to regulate their behavior in an effective and healthy way.

Interventions addressing incarcerated youth behavior: A review of literature.

Many youth who are incarcerated have serious behavioral problems that negatively affect their ability to benefit from educational services. In the Guiding Principles for Providing High-Quality Education in Juvenile Justice Secure Care Settings published at the end of 2014, the U.S. Departments of Justice and Education address this issue in Principle 1, which asserts the importance of ensuring the safety and well-being of youth who are incarcerated. To address the research progress since publication of the Guiding Principles, ProQuest and Ebsco were systematically searched and a hand search was conducted. Ten intervention studies were identified that addressed youth behavior. The studies primarily employed a single subject research design and focused on increasing youth compliance and/or decreasing disruptive behaviors. Reviewed studies provide support for providing clear behavioral expectations and reminders, praise, and reinforcement, as well as cognitive-behavioral interventions. However, methodological limitations, including the lack of treatment integrity in nine of the studies, limit conclusions. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Hypoxia as a driver of resistance to immunotherapy.

Hypoxia, a hallmark of solid tumors, determines the selection of invasive and aggressive malignant clones displaying resistance to radiotherapy, conventional chemotherapy or targeted therapy. The recent introduction of immunotherapy, based on immune checkpoint inhibitors (ICPIs) and chimeric antigen receptor (CAR) T-cells, has markedly transformed the prognosis in some tumors but also revealed the existence of intrinsic or acquired drug resistance. In the current review we highlight hypoxia as a culprit of immunotherapy failure. Indeed, multiple metabolic cross talks between tumor and stromal cells determine the prevalence of immunosuppressive populations within the hypoxic tumor microenvironment and confer upon tumor cells resistance to ICPIs and CAR T-cells. Notably, hypoxia-triggered angiogenesis causes immunosuppression, adding another piece to the puzzle of hypoxia-induced immunoresistance. If these factors concurrently contribute to the resistance to immunotherapy, they also unveil an unexpected Achille's heel of hypoxic tumors, providing the basis for innovative combination therapies that may rescue the efficacy of ICPIs and CAR T-cells. Although these treatments reveal both a bright side and a dark side in terms of efficacy and safety in clinical trials, they represent the future solution to enhance the efficacy of immunotherapy against hypoxic and therapy-resistant solid tumors.

Serological prevalence of SARS-CoV-2 infection and associated factors in healthcare workers in a "non-COVID" hospital in Mexico City.

BACKGROUND: Mexico is one of the countries with the highest number of deaths from the COVID-19 pandemic. In spite of this high mortality, in Mexico the number of confirmed cases and diagnostic tests per million population are lower than for other comparable countries, which leads to uncertainty about the actual extent of the pandemic. In Mexico City, healthcare workers represent an important fraction of individuals with SARS-CoV-2 infection. We performed a cross-sectional study whose objective was to estimate the frequency of antibodies to SARS-CoV-2 and identify associated factors in healthcare workers at a large hospital in Mexico City. METHODS: We conducted a serological survey in a non-COVID national referral teaching hospital. The study population included all the personnel that works, in any capacity, in the hospital. From this population we selected a representative sample of 300 individuals. Blood samples were collected and questionnaires were applied between August 10th and September 9th, 2020. RESULTS: ELISA results indicate a serological prevalence of SARS-CoV-2 infection of 13.0%. Working in the janitorial and security groups, having an educational level below a university degree, and living with a larger number of people, were all identified as sociodemographic factors that increase the probability of having SARS-CoV-2 infection. CONCLUSIONS: Less favored socioeconomic groups face significantly higher prospects of experiencing SARS-CoV-2 infection and in institutions such as ours, providing janitorial and security workgroups with additional testing and counseling could help to limit the spread of contagion. The rate from the official number of confirmed cases in Mexico City is substantially smaller than the seropositive rate identified in this work.

Grupos de acogimiento: Sistematización de un modelo participativo para la formulación diagnóstica y terapéutica en un centro de salud mental comunitaria. Chorrillos-Perú, 2018 / Foster groups: Systematization of a participatory model for the diagnostic and therapeutic formulation in a community mental health center. Chorrillos-Peru, 2018

RESUMEN Objetivo: Sistematizar el proceso de formulación de diagnósticos y planes de tratamiento en grupos participativos de profesionales y usuarios, denominados Grupos de Acogimiento, como alternativa al modelo convencional de consultas psiquiátricas individuales y secuenciales, en el ámbito de la salud mental comunitaria. Material Métodos : El estudio se realizó en un centro de salud mental comunitaria (CSMC) de Lima durante el periodo enero-diciembre de 2018. Se siguió una metodología de investigación-acción en la que propuestas surgidas de la experiencia se implementaban para, luego, analizar críticamente sus resultados y ajustarlos sucesivamente hasta lograr un punto de estandarización o de consenso entre los participantes. Resultados: El modelo participativo se aplicó en 97% de los usuarios durante el periodo de observación y se llegó a la formulación de diagnósticos y planes de tratamiento durante la sesión del Grupo de Acogimiento, en el 85% de los pacientes. Las sesiones se estandarizaron en cuatro momentos concatenados y semi-estructurados: Presentación horizontal de participantes, comprensión empática mutua, exploración colectiva de causas, y decisiones terapéuticas compartidas. Conclusiones: El modelo participativo de Grupos de Acogimiento fue una alternativa viable y eficaz para la formulación inicial de diagnósticos y planes terapéuticos en el CSMC, con fases semiestructuradas convenientemente estandarizadas y replicables.

SUMMARY Objective: To systematize the process of diagnosis and treatment plan formulations in participatory groups of users and professionals, called Fostering Groups, as an alternative to the conventional model of individual and sequential psychiatric consults, in the field of community mental health. Material and Methods : The study was carried out in a community mental health center (CMHC) in Lima throughout the period January-December 2018. An action-research methodology was followed, in which proposals arising from the experiences were put into action to, then, critically analyze the and adjust them successively until reaching a point of standardization or consensus among the participants. Results : The participatory model was applied in 97% of the users during the observation period and the formulation of diagnoses and treatment plans during the Foster Group session was successfully reached in 85% of the patients. The sessions were standardized in four concatenated and semi-structured moments: Horizontal presentation of participants, mutual empathetic understanding, collective exploration of causes, and shared therapeutic decisions. Conclusions : The Fostering group model was a viable and effective alternative for the initial formulation of diagnoses and therapeutic plans in the CMHC, with semi-structured phases conveniently standardized and replicable.

Complicaciones crónicas en pacientes con diagnóstico reciente de diabetes mellitus tipo 2 / Chronic complications in patients recently diagnosed with type 2 diabetes mellitus

RESUMEN La diabetes mellitus tipo 2 representa un problema de salud actual con altos índices de morbilidad y mortalidad a escala mundial. Se realizó un estudio descriptivo, transversal y observacional con 200 pacientes que presentaban esta afección en el Hospital Universitario Clínico-Quirúrgico Arnaldo Milián Castro, Santa Clara, de enero a diciembre 2017, con el objetivo de describir las complicaciones crónicas en este tipo de paciente. El 80 % de los diagnósticos se realizó por glucemias en ayunas. Se detectó la presencia de complicaciones crónicas y enfermedades concomitantes, como: polineuropatía diabética, enfermedades de la piel y esteatohepatitis no alcohólica. Aunque el 100 % de los pacientes presentó algún factor de riesgo, la enfermedad no fue pesquisada precozmente; se detectó por exámenes indicados por otras causas y se realizó un diagnóstico tardío, en presencia de complicaciones crónicas. Se evidenció la necesidad del trabajo de prevención y promoción de salud, a fin de evitar las tasas de morbilidad y expansión de la enfermedad.

ABSTRACT Type 2 diabetes mellitus represents a current health problem with high morbidity and mortality rates worldwide. A descriptive, cross-sectional and observational study with 200 patients recently diagnosed with type 2 diabetes mellitus and admitted at "Arnaldo Milián Castro" Clinico-Surgical University Hospital in Santa Clara was done from January to December 2017 with the objective of describing chronic complications in this type of patient. The 80 % of the diagnoses was confirmed by fasting blood sugar tests. The presence of chronic complications and concomitant diseases such as diabetic polyneuropathy, skin diseases and non-alcoholic steatohepatitis were detected. Despite 100 % of patients had some risk factor, the disease was not diagnosed early; it was detected through exams indicated for other reasons and a late diagnosis was done when chronic complications appear. The necessity to health prevention and promotion activities was evidenced in order to avoid morbidity rates, as well as, the spread of the disease.

Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing / Caracterización de las regiones no traducidas ARN [UTR] de las isoformas de Trypanosoma cruzi LYT1 derivadas de un trans-empalme alternativo / Caracterização das regiões não-traduzidas do RNA [UTR] das isoformas de Trypanosoma cruzi LYT1 derivadas de uma junção trans-alternativa

Abstract In trypanosomatids, gene expression is mainly regulated at posttranscriptional level, through mechanisms based on the interaction between RNA Binding Proteins [RBPs] and motifs present in the untranslated regions [UTRs] of the mRNAs, which altogether form ribonucleoproteic complexes [RNP] that define the fate of the mRNA. The pre-mRNA derived from the LYT1 gene of Trypanosoma cruzi, is processed by alternative trans-splicing, resulting in different mRNAs which code for the isoforms mLYTl and kLYTl, proteins having differential expression, cellular location and function. The aim of this study was to characterize the 5' and 3' UTRs of the LYT1 mRNAs as the initial step towards the objective of identification of the RBPs responsible for their differential expression. The presence of the two types of 5' UTRs were confirmed in two T. cruzi isolates belonging to the DTU I, thus, corroborating the occurrence of alternative trans-splicing also in the LYT1 gene of this T. cruzi DTU. In addition, for the first time, was unscovered the existence of two types of LYT1 mRNAs transcripts, differing in length by 116 nts, that are generated by alternative polyadenylation. Furthermore, an in-silico analysis of the experimentally obtained UTRs, and ten additional LYT1 sequences retrieved from TritrypDB and GenBank databases, together with a thoroughly search of structural motifs, showed a remarkable conservation of relevant structural motifs previously associated with RNA metabolism in the different UTRs; these elements might be involved in the differential stage-specific expression of each LYT1 isoform.

Resumen En los trypanosomátidos, la expresión génica se regula principalmente en el nivel post-transcripcional mediante mecanismos basados en la interacción entre las proteínas de unión del ARN [RBP] y las figuras presentes en las regiones no traducidas [UTR] de las ARN, que en conjunto forman complejos ribonucleoproteicos [RNP] que definen el destino de la ARN. El pre-ARN derivado del gen LYT1 del Trypanosoma cruzi es procesado por trans-empalme alternativo, dando como resultado diferentes ARN que codifican las isoformas mLYTl y kLYTl, proteínas con expresión diferencial, localización celular y función. El objetivo de este estudio fue caracterizar los 5' y 3' UTR de las ARN LYT1 como el paso inicial hacia la identificación de los RPB responsables de la expresión diferencial. Se confirmó la presencia de los dos tipos de 5' UTR en dos aislantes del T. cruzi pertenecientes al DTU I; de esta forma también se comprobó la ocurrencia del trans-empalme alternativo en el gen LYT1 de este T. cruzi DTU. Además, por primera vez, se pudo demostrar la existencia de dos tipos de transcripciones de ARN LYT1, que difieren en longitud por 116 nts, y son generadas por poliadenilación alternativa. Adicionalmente, se realizó un análisis in-silico de la UTR obtenida experimentalmente, y otras diez secuencias LYT1 recuperadas de las bases de datos TritrypDB y GenBank, junto con una búsqueda exhaustiva de figuras estructuradas, mostrando una notable conservación de los figuras estructurales asociadas con el metabolismo del ARN en los diferentes UTR; estos elementos podrían estar implicados en la expresión diferenciada de la etapa específica de cada isoforma LYT1.

Resumo Nos tripanossomatídeos, a expressão génica é regulada principalmente a nível pós-transcricional mediante mecanismos baseados na interação entre as proteínas de união do RNA [RBPs] e as fugiras presentes nas regiões não-traduzidas [UTRs] do RNA. O pré-RNA derivado do gene LYT1 do Trypanosoma cruzí é processado por uma junção trans-alternativa, resultando em diferentes RNA que codificam as isoformas mLYTl e kLYTl, proteínas com expressão, localização celular e função diferenciadas. O objetivo de este estudo foi caracterizar as 5' e 3' UTRs dos RNAs LYT1 como sendo o passo inicial na identificação das RBPs responsáveis pela expressão diferenciada. A presença dos dois tipos de 5' UTRs foi confirmada em dois isolados de T. cruzí pertencentes ao DTU I; corroborando assim com a ocorrência da junção trans-alternativa no gene LYT1 de este T. crují DTU. Adicionalmente, se demonstrou pela primeira vez a existência de dois tipos de transcrições de RNA LYT1, que se diferenciam em comprimento por 116 nts, e são geradas por poliadenização alternativa. Além disso, realizou-se uma análise in-sílico da UTR obtida experimentalmente e outras dez sequencias LYT1 recuperadas das bases de dados TritrypDB e GenBank, junto com uma busca exaustiva de figuras estruturadas, mostrando uma notável conservação das figuras estruturais associadas com o metabolismo do RNA nas diferentes UTRs. Estes elementos poderiam estar envolvidos na expressão estágio-específica diferenciada de cada isoforma LYT1.

Effect of breastfeeding on postpartum depressive symptoms among adolescent and young adult mothers.

PURPOSE: To describe the association between breastfeeding and postpartum depressive symptoms among a sample of adolescent and young adult mothers and to determine whether breastfeeding difficulty moderates this association. MATERIALS AND METHODS: Data were derived from a prospective cohort of pregnant adolescent and young adult females (ages 14-21) as they transitioned to parenthood. This analysis uses data collected during pregnancy and at 6 months postpartum among mothers (n = 137) who initiated breastfeeding. Multivariable linear regression was used to adjust for prenatal depressive symptoms and other potential confounders. RESULTS: Postpartum depressive symptoms were not significantly associated with breastfeeding duration or breastfeeding at 6 months. Early breastfeeding difficulty moderated the association between depressive symptoms and breastfeeding at 6 months. Among young mothers who were still breastfeeding at 6 months, those who reported no early breastfeeding difficulties had the lowest depressive scores and those who reported much early breastfeeding difficulty had the highest depressive scores at 6 months. CONCLUSIONS: Minimizing challenges with breastfeeding may help improve postpartum mental health among adolescent and young adult mothers. Health care providers should help young pregnant women manage expectations about breastfeeding and ensure that they are linked to appropriate professional breastfeeding support during the early postpartum period.

Trauma symptoms in a diverse population of sexually abused children.

OBJECTIVE: The role of ethnicity, age, and gender in studies of sexually abused children has been overlooked. Yet, studies have found that African American and Latino sexually abused children suffer from more severe symptoms at treatment presentation and longer lasting effects than Caucasian children. Furthermore, evidence suggests that age and gender impact symptoms resulting from sexual abuse. This study focused on examining demographic differences in trauma symptoms and posttreatment data in an urban, ethnically diverse population of sexually abused children. METHOD: The sample was selected from archival data of 176 children who ranged from the ages of 8 to 16. Therapists administered the Trauma Symptom Checklist for Children (TSCC) at pretreatment and posttreatment. RESULTS: Symptom scores were significantly lower for 41 children who completed 3 months of trauma-focused cognitive-behavioral therapy (TF-CBT) on all TSCC scales except Sexual Concerns, Dissociation, and Overt Dissociation. For the entire sample, mean symptom scores for Sexual Preoccupation were significantly higher for African American children compared to Latino and Caucasian children. Older children had significantly higher scores on scales for Anxiety, Depression, Anger, Posttraumatic Stress, Dissociation, and Sexual Distress. Sexual Distress scale scores were significantly higher among females. CONCLUSION: Findings indicate that trauma symptoms significantly improved after 3 months of TF-CBT. Trauma symptoms differed for children by ethnic group, age, and gender. Identifying factors that impact symptomatology is critical to providing effective, culturally competent treatment for ethnic minority children who are child sexual abuse victims. (PsycINFO Database Record

Comparison of hemocytes of V-instar nymphs of Rhodniusprolixus (Stål) and Rhodnius robustus (Larousse 1927), before and after molting / Comparación de los hemocitos de las ninfas de V instar de Rhodnius prolixus (Stål) and Rhodnius robustus, antes y después de la muda (Larousse 1927)

Background. The immune response of insects involves humoral factors and cellular elements known as hemocytes. There are different reports that explore the response of hemocytes to infections, but the effect that molting has on this response has not been explored so far. We hypothesized that there would be a change in the percentage of hemocytes as a response to the molting process. Objective. The aim of this work was to compare the hemogram (CBC), the formula, and the differential count of hemocytes in IV instar before molting and in V instar nymphs 24 hours after molting in two species: Rhodnius prolixus and Rhodnius robustus. Materials and methods. We assayed different staining methodologies including Giemsa, Alcian Blue pH 2.0, Alcian Blue pH 2.6, Gomori substrate, PAS (Schiff), Sudan Black and Papanicolau with positive controls for each one. In the Gomori staining, we observed lysosomes in the granulocytes and plasmatocytes, but the differentiation was better detected using Giemsa staining. Results. There were no statistically significant differences between the two species studied in plasmatocytes (p=0,053) or even in granulocytes (p=0,5). However, differences were significant in the prohemocytes (p=0,001) during the molting process in both Rhodnius prolixus and Rhodnius robustus. Conclusions. Significant differences in prohemocytes between nymphs of IV and V instar were detected. No significant differences in the amount of cells were observed between the two species and the two stages. These findings may be explained due to their role as precursor cell of prohemocytes.

Antecedentes. La respuesta inmune de los insectos involucra factores humorales y elementos celulares llamados hemocitos. Existen varios trabajos explorando la respuesta de los hemocitos frente a infecciones, pero no como respuesta al proceso de muda. Objetivo. Comparar el hemograma: la fórmula y el recuento diferencial de hemocitos de Rhodnius prolixus y Rhodnius robustus en ninfas de IV estadio antes de la muda y ninfas de V estadio 24 horas después de la muda. Materiales y métodos. Se ensayaron las coloraciones Giemsa, Alcian Blue pH 2.0, Alcian Blue pH 2.6, sustrato Gomori, PAS (Schiff), Sudán Negro, Papanicolau, con controles positivos para cada una. Con la coloración de Gomori se observaron lisosomas en los granulocitos y en plasmatocitos, pero la diferenciación se logró mejor con la coloración de Giemsa. Resultados. Los plasmatocitos fueron más abundantes en las ninfas de IV estadio de Rhodnius robustus y en las de V estadio de las dos especies, pero en las ninfas de V estadio de R. prolixus, el recuento de prohemocitos fue mayor; para los oenocitoides, esferulocitos y adipohemocitos el recuento no alcanzó al 1 %. En las dos especies no se encontraron diferencias estadísticamente significativas en plasmatocitos (p=0,05319), ni en granulocitos (p=0,5), entre las ninfas de IV y V estadio, pero fue significativa en prohemocitos (p=0,001). Conclusiones. Se detectaron diferencias significativas en prohemocitos entre las ninfas de IV y V estadio. No hubo un aumento significativo en el conteo de células en los diferentes estadios.

Modulation of GABA-A receptors of astrocytes and STC-1 cells by taurine structural analogs.

Taurine activates and modulates GABA receptors in vivo as well as those expressed in heterologous systems. This study aimed to determine whether the structural analogs of taurine: homotaurine and hypotaurine, have the ability to activate GABA-A receptors that include GABAρ subunits. The expression of GABA-A receptors containing GABAρ has been reported in the STC-1 cells and astrocytes. In both cell types, taurine, homo-, and hypotaurine gated with low efficiency a picrotoxin-sensitive GABA-A receptor. The known bimodal modulatory effect of taurine on GABAρ receptors was not observed; however, differences between the activation and deactivation rates were detected when they were perfused together with GABA. In silico docking simulations suggested that taurine, hypo-, and homotaurine do not form a cation-π interaction such as that generated by GABA in the agonist-binding site of GABAρ. This observation complements the electrophysiological data suggesting that taurine and its analogs act as partial agonists of GABA-A receptors. All the observations above suggest that the structural analogs of taurine are partial agonists of GABA-A receptors that occupy the agonist-binding site, but their structures do not allow the proper interaction with the receptor to fully gate its Cl(-) channel.

Small nuclear ribonucleoprotein polypeptide N quantitative methylation analysis in infants with central hypotonia.

Central hypotonic is one of the most difficult issues in neurology, ruling out neurogenetic syndromic causes is critical, Prader-Willi syndrome (PWS) it is the most frequent genetic syndrome, it is caused by the loss of expression of the paternal allele in a group of imprinted genes within 15q11-q13, and is characterized by severe prenatal and postnatal hypotonia. SNURF-SNRPN gene methylation detects 99% of the cases but fluorescent in situ hybridization (FISH) analysis is necessary to confirm chromosome microdeletions. The advantage of SNRP-quantitative strategy of methylated alleles is that it makes it possible to make the diagnosis and identify deletions and mosaicism in one reaction. In infants clinical diagnosis is difficult. It has been proposed that around 40% of hypotonic patients have PWS but an accurate percentage has not been established. Twenty-four central hypotonic infants were studied by this molecular strategy, showing 41.5% with the disease. This molecular approach also permitted calculation of gene dosage and detection of those cases with microdeletion.

Informe de actividades salud y nutrición meses junio. julio y agosto 1995 / Report of activities health and nutrition months june. july and august 1995

Para el efecto del proyecto se concidero nesesario realizar en primera instacia un taller de analisis de oferta de productos locales en forma conjunta con productores de productos alimenticios nacionales, se concideraron como alternativas productos lacteos y mezclas de leguminosas y sereales a ser utilizados en el desayuno, almuerzo y merienda...