1.
Rev Esp Fisiol
; 33(3): 181-6, 1977 Sep.
Article
in English
| MEDLINE
| ID: mdl-302459
ABSTRACT
The capacity of human foetal lymphocytes to respond to PHA and to form E-rosettes have been compared with data from adult individuals. For this purpose a microculture system that uses whole blood and avoids the problems of lymphocyte separation, has been developed. Foetal lymphocytes reached optimal stimulation with lower dosis of PHA (31,2 microgram/ml) as compared with adult cells (125-252 microgram/ml). However their quantitative response (measured by 14C-thymidine uptake) was equal in both groups. In addition, peripheral T cells (E-rosetting cells) reached values of 36.47 +/- 9% in newborn and 49.6 +/- 10% in normal adult controls. These results are discussed as to the status and development of cellular inmunity in human foetus.
Subject(s)
Fetal Blood/physiology , Immunity, Cellular , Lectins/pharmacology , T-Lymphocytes/immunology , Adult , Blood Cell Count , Culture Techniques , Fetal Blood/immunology , Humans , Immunity, Cellular/drug effects , Infant, Newborn , Lymphocyte Activation/drug effects , Microchemistry/methods , Stimulation, Chemical , T-Lymphocytes/physiology
2.
Sangre (Barc)
; 22(6): 949-52, 1977.
Article
in Spanish
| MEDLINE
| ID: mdl-601672