Subject(s)
Agglutination Tests , Precipitin Tests , Typhoid Fever/diagnosis , Adolescent , Antibodies, Bacterial/analysis , Antigen-Antibody Reactions , Antigens, Bacterial/immunology , Child , Child, Preschool , Enzyme-Linked Immunosorbent Assay , Hemagglutination Tests , Humans , Salmonella typhi/immunology , Typhoid Fever/bloodSubject(s)
Forensic Medicine , Serology/methods , Animals , Blood Stains , Cattle , Cells/immunology , Histocompatibility Testing , HumansSubject(s)
Antigens/administration & dosage , Down Syndrome/therapy , Immunotherapy , Adolescent , Antigens/immunology , Child , Child, Preschool , Down Syndrome/immunology , Female , Humans , Infant , MaleABSTRACT
Se describe una aplicacion del metodo de fijacion en superficie y retencion del colorante para reconocer la presencia de anticuerpos y antigeno circulante en el suero de pacientes oncocercosos. La especificidad de la prueba se confirma por haber observadp reacciones negativas empleando sueros de diversas condiciones clinicas prevalentes en el Distrito Federal, quedando pendientes investigaciones con suero de pacientes no oncocercosos de las mismas zonas tropicales donde la filariasis a que nos referimos ha sido endemica
Subject(s)
Humans , Hematologic Tests , OnchocerciasisABSTRACT
El aislamiento de substancias de propriedades antigenicas especificas de la orina de ninos con sindrome de Down, sugirio la conveniencia de aplicarlo, con fines terapeuticos, administrando, por via subcutanea, dosis adecuadas. Los resultados han sido sorprendentes, especialmente en pacientes menores de dos anos; discretos en los de dos o cinco y menos aparentes en mayores de 8 anos. Se considera que el tratamiento antigenico puede ser util adicion a los recursos empleados en la rehabilitacion de estos pacientes
Subject(s)
Infant , Child, Preschool , Child , Adolescent , Adult , Humans , Male , Female , Antigens , Down Syndrome , UrineABSTRACT
De la orina de pacientes de cronicidad limitada o permanente, ha sido posible aislar substancias de propiedad antigenica especifica para cada padecimiento las que en mezcla con suero de los mismos enfermos o de otros que sufren el mismo padecimiento, en pruebas de fijacion en superficie, dan lugar a reacciones de tipo antigenoanticuerpo. Por lo tanto, consideramos que lo que designamos como urodiagnostico, puede ser aconsejado como practica adicional a tantos otros procedimientos emergentes de laboratorio
Subject(s)
Humans , Antigens , Clinical Laboratory Techniques , UrineSubject(s)
Schizophrenia/immunology , Antibodies/analysis , Antigens/analysis , Autoantibodies/analysis , Female , Humans , MaleABSTRACT
By means of the surface and color fixation test specific cross-reactions were detected in mixtures of serum from two patients suffering from the same type of neoplasia, and it was of interest that tests with 105 sera from well proven cases produced reactions which were consistent with the clinical and pathological reports.
Subject(s)
Cross Reactions , Neoplasms/immunology , Bromphenol Blue , Child , Humans , Lymphoma/immunology , Neoplasms/blood , Sarcoma/immunologyABSTRACT
Por medio de la prueba de fijacion y retencion del colorante ha sido posible reconocer reacciones inmunologicas en mezclas de sueros provenientes del mismo tipo de cancer, siendo de interes que tales resultados no solo fueron confirmados por pruebas especificas empleando los antigenos correspondientes, sino que al menos los 105 casos estudiados fueron obtenidos de instituciones responsables
Subject(s)
Cross Reactions , NeoplasmsABSTRACT
The surface fixation method has been found to be a reliable procedure for detection of antibodies of retrogenetic origin in cancer serum and also for specific immunoglobulins stimulated by antigens sinthezized in malignant cells. An insoluble extract obtained from human placentas has been used for detection of retrogenectic antibodies and with soluble substances obtained from the urine of patients it has been possible to detect what seem to be specific antibodies in retinoblastoma, Hodgkin, sarcoma and carcinoma. However with a urine extract from leukemia patients the positive reactions occur with leukemia serum from leukemia as well as with the related lymphoma and myeloma. Circulating tumor associated antigens can be detected in mixtures of an anti-fetal serum with cancer serum, but cross-reactions have been found in similar tests with pregnant women's serum.
Subject(s)
Antibodies, Neoplasm/analysis , Antigen-Antibody Reactions , Antigens, Surface/immunology , Neoplasms/immunology , Antigens, Surface/analysis , Female , Humans , Latex Fixation Tests/methods , Peptides , PregnancyABSTRACT
An attempt has been made to use the rapid surface fixation method for the investigation of immunological reactions in mixtures of sera from mothers and their mongoloid children. This preliminary report has been encouraging since all positive reactions were properly controlled with the serum of patients suffering from various clinical conditions but free of Down's syndrome. A water soluble substance was isolated from the urine of patients which produced positive reactions like those performed with the serum of mongoloid children.
Subject(s)
Down Syndrome/immunology , Fetal Diseases/immunology , Antigen-Antibody Reactions , Antigens, Surface/urine , Complement Fixation Tests , Down Syndrome/genetics , Female , Humans , Maternal-Fetal Exchange , Pregnancy , Prenatal Diagnosis/methodsABSTRACT
An attempt to use the "surface fixation" method for the detection of antigen-antibody reactions with VDRL and Treponema pallidum has given results which corroborate conventional serological findings in patients suspected of being infected with this organism. The test is performed over strips of filter paper in which mixtures of the serum with the antigen are deposited and submitted to a special procedure of staining in order to detect antigen-antibody reactions.
Subject(s)
Antigens, Bacterial/analysis , Antigens, Surface/analysis , Syphilis Serodiagnosis , Syphilis/diagnosis , Treponema pallidum/immunology , Antigen-Antibody Reactions , Fluorescent Antibody Technique/methods , Humans , Syphilis/immunologyABSTRACT
Surface fixation has been used to detect antigen antibody-reactions with bacteria, protozooa and in some instances with drugs considered responsible of adverse reactions attributed to allergy. The application of this method to penicillin is reported. The test has been positive with serum from well-proven cases of hypersensitivity but also in subjects that had undergone penicillin treatment. So far, no comparative observations with conventional tests have been made; however, if the presence of circulating antibody is considered the cause of adverse reactions to penicillin, this simple test may have clinical usefulness.
Subject(s)
Antigen-Antibody Reactions , Drug Hypersensitivity/immunology , Penicillins/immunology , Humans , Skin Tests , Surface PropertiesABSTRACT
Surface fixation is a rapid and simple method for the detection of antigen-antibody reactions. It has been used as an epidemiologic and diagnostic criterium for brucellosis, typhus and typhoid fever and recently found to be an encouraging test for amebiasis. Data concerning its application in toxoplasmosis has been considered a justified addition to the more complicated procedures used for the diagnosis of this infection, which has become a world-wide problem. It may thus be used as a screening test, as well as a simplified method of titration of postive sera.
