ABSTRACT
OBJECTIVE: To investigate the differences of mRNA quantitation and protein expression of vascular growth factors including platelet-derived endothelial cell growth factor (PD-ECGF) and vascular endothelial growth factor (VEGF) in intestinal tissues in colorectal carcinoma patients with and without schistosomiasis. METHODS: Thirty colorectal carcinoma patients with schistosomiasis and 30 colorectal carcinoma patients without schistosomiasis were included in this study. The mRNA quantitation and protein expression of PD-ECGF and VEGF in the normal tissue, peri-carcinoma tissue as well as carcinoma tissue obtained from surgical specimens were detected by qRT-PCR and Western blot. RESULTS: The mRNA relative quantitations of PD-ECGF in normal tissue, peri-carcinoma tissue and carcinoma tissue in the colorectal carcinoma patients with schistosomiasis were 1.726, 1.766 and 2.729 times to those in the colorectal carcinoma patients without schistosomiasis, respectively. The corresponding ones of VEGF were 2.138, 1.831 and 3.376 times, respectively. The protein expression levels of PD-ECGF and VEGF in normal tissue, peri-carcinoma tissue and carcinoma tissue were higher in the colorectal carcinoma patients with schistosomiasis than in the colorectal carcinoma patients without schistosomiasis. CONCLUSIONS: The expressions of vascular growth factors including PD-ECGF and VEGF are higher in the colorectal carcinoma patients with schistosomiasis than in the colorectal carcinoma patients without schistosomiasis. Therefore, schistosomiasis may be one of the risk factors of colorectal cancer.
Subject(s)
Colorectal Neoplasms/genetics , Intestinal Mucosa/metabolism , Schistosomiasis japonica/genetics , Thymidine Phosphorylase/genetics , Vascular Endothelial Growth Factor A/genetics , Adult , Aged , Animals , Colorectal Neoplasms/metabolism , Colorectal Neoplasms/parasitology , Female , Gene Expression , Humans , Male , Middle Aged , Schistosoma japonicum/isolation & purification , Schistosoma japonicum/physiology , Schistosomiasis japonica/metabolism , Schistosomiasis japonica/parasitology , Thymidine Phosphorylase/metabolism , Vascular Endothelial Growth Factor A/metabolismABSTRACT
OBJECTIVE: To investigate the epidemic pattern, diagnosis and treatment of schistosomiasis after the 16-years intervention program on its transmission in Jiaxing region of Zhejiang province. METHODS: Clinical data of schistosomiasis patients during the last 10 years and pathological specimens with deposited schistosomal eggs during the last 8 years were retrospectively analyzed. RESULTS: The total numbers of schistosomiasis patients admitted to hospital increased from 194 in 2001 to 960 in 2010, and from 78 to 266 with complications. Number of the ones with accompanied diseases increased from 116 to 694. All the numbers of the above said three groups showed an yearly increase. The hardest hit age of the patients was between 60 and 74. The number of specimens with deposited schistosomal eggs increased from 192 in 2003 to 298 in 2010. While the ratio of specimens with deposited schistosomal eggs to the total number of pathological specimens became slightly decreasing, the average age of patients increased. Eggs were mainly deposited on appendix, colon, rectum, stomach, liver, gallbladder and small intestine. CONCLUSION: Schistosomiasis still hit the Jiaxing region, with the average age of patients increased.
