[Enantiomeric separation of N-FMOC amino acids with sulfobutyl ether-beta-cyclodextrin as chiral additive by capillary electrokinetic chromatography].

Enantiomeric separation of N-FMOC amino acids by capillary electrophoresis with an anionic chiral resolving agent, sulfobutyl ether-beta-cyclodextrin (SBE-beta-CD) with average substitution degree of 3.8, employed as chiral additive was studied. Chiral separation was achieved with the mode of electrokinetic chromatography due to the electrophoretic mobility and chiral complexing ability of the chiral additive. The effects of pH of background electrolyte, the concentration of sulfobutyl ether-beta-cyclodextrin on enantiomeric separations were investigated. The enantiomeric separations of 8 N-FMOC amino acids were accomplished, among which 5 N-FMOC amino acids could be on baseline separated.

[Synthesis and application of sulfonated beta-cyclodextrins as chiral additives in capillary electrophoresis].

Three new kinds of the sodium salt of sulfonated beta-cyclodextrins with different degrees of substitution were synthesized and characterized. A simple synthesis method was employed by direct sulfonating reaction with concentrated sulfuric acid. Sulfonated beta-cyclodextrins were used as chiral resolving agents for the capillary electrophoretic separation of enantiomers in high pH and low pH background electrolytes. In different electrophoretic polarity mode, the effects of the type and concentration of sulfonated beta-cyclodextrin were investigated. Sulfonated beta-cyclodextrins were proved to be strong complexing agents for basic and neutral analytes.