Comparison of sound touch elastography and quantification for assessing the renal pathologic changes in patients with proteinuria.

OBJECTIVE: Sound touch elastography (STE) and sound touch quantification (STQ) are novel imaging methods to evaluate tissue stiffness. This study aims to investigate renal stiffness in patients with chronic kidney disease (CKD) by STE and STQ, using renal biopsy as 'gold standard'. METHODS: Between 2019 January and 2022 June, 60 patients who underwent renal biopsy for proteinuria (cases) and 45 healthy volunteers (controls) at our hospital were included in this study. The maximum and mean elastic modulus (Emax, Emean) of region of interest in right kidney were measured by STE and STQ techniques. Biochemical profiles and renal biopsy findings were recorded. RESULTS: Both Emax and Emean measured by STE were significantly different between cases and controls. ROC analysis of STE measurements revealed using a cutoff of 13.53 kPa for Emax and 10.16 kPa for Emean, the area under the curve (AUC) to distinguish nephropathy from healthy was 0.718 and 0.744. Analysis of ROC for STQ measurements showed that using a cutoff value of 15.87 kPa for Emax and 9.95 kPa for Emean, the AUC for the nephropathy was 0.612 and 0.569. Emax and Emean values were significantly different among CKD patients with mild, moderate and severe pathological stage. The Emax value for STE was positively related to Scr, ß2-MG (r = 0.257, 0.292, p < 0.05). CONCLUSION: Both STE and STQ are non-invasive, feasible methods to quantitatively evaluate renal stiffness. STE is more effective than STQ in the diagnosis of CKD patients with proteinuria. CRITICAL RELEVANCE STATEMENT: Sound touch elastography is more effective than sound touch quantification in the diagnosis of chronic kidney disease patients with proteinuria. KEY POINTS: • Emax and Emean measured by STE were different between cases and controls. • Emax and Emean were different among CKD patients with different pathological stages. • The Emax value for STE was positively related to serum creatinine, ß2-microglobulin.

Evaluation of internal and shell stiffness in the differential diagnosis of breast non-mass lesions by shear wave elastography.

BACKGROUND: The diagnostic specificity of conventional ultrasound for breast non-mass lesions (NMLs) is low at approximately 21%-43%. Shear wave elastography (SWE) can distinguish benign from malignant lesions by evaluating the internal and peripheral stiffness. SWE has good reproducibility and high diagnostic efficacy. However, there are very few independent studies on the diagnostic value of SWE in breast NMLs. AIM: To determine the value of SWE in the differential diagnosis of breast NMLs. METHODS: This study enrolled a total of 118 patients with breast NMLs who underwent SWE examinations in the Beijing Shijitan Hospital Affiliated to Capital Medical University and The Second Hospital of Shandong University from January 2019 to January 2020. The internal elastic parameters of the lesions were recorded, including maximum (Emax), mean (Emean) and minimum elastic values and the standard deviation. The following peripheral parameters were noted: Presence of a "stiff rim" sign; Emax, and Emean elasticity values within 1 mm, 1.5 mm, 2 mm, 2.5 mm and 3 mm from the edge of NMLs. The receiver operating characteristic curve of each parameter was drawn, and the areas under the curve were calculated. RESULTS: Emax, Emean and elastic values, and the standard deviation of the internal elastic values in malignant NMLs were significantly higher than those in benign NMLs (P < 0.05). The percentage with the "stiff rim" sign in malignant NMLs was significantly higher than that in the benign group (P < 0.05), and Emax and Emean at the shell of 1 mm, 1.5 mm, 2 mm, 2.5 mm and 3 mm in the malignant group were all higher than those in the benign group (P < 0.05). Of the surrounding elasticity values, Emax of the shell at 2.5 mm in malignant NMLs had maximum areas under the curve of 0.900, and the corresponding sensitivity and specificity were 94.57% and 85.86%, respectively. CONCLUSION: The "stiff rim" sign and multiple quantitative elastic values within and around the lesion had good diagnostic performance in the differential diagnosis of breast NMLs. Emax in peripheral tissue had better diagnostic efficiency than other parameters.

Effects of Rex-bypass shunt on the cavernous transformation of the portal vein in children: evaluation by the color Doppler ultrasonography.

BACKGROUND: The study was to investigate the role of color Doppler ultrasonography in the evaluation of the effect of Rex-bypass shunt on the cavernous transformation of the portal vein (CTPV) in children. METHODS: Fifty children with symptomatic extrahepatic portal hypertension who received Rex-bypass shunt were retrospectively reviewed, and they were diagnosed with CTPV by ultrasonography. The clinical characteristics were analyzed before and after operation. RESULTS: Forty-five patients received color Doppler ultrasonography at 6 months after surgery, and good patency in the bypass vessels was displayed. The platelet count significantly increased (P < 0.001) and the esophagogastric varices were improved significantly (p < 0.001). The patency of bypass vessels on color Doppler ultrasonography was consistent with the changes in the platelet count and the degree of esophagogastric varices on gastroscopy before and after operation. The diameter of bypass vessels at 6 months was slightly larger than that at 7 days after operation, and there was no significant difference in blood flow velocity between two time points (P = 0.507). CONCLUSIONS: Color Doppler ultrasonography can display the patency, diameter, and flow velocity of bypass vessels. It plays an important role in evaluating the effect of Rex-bypass shunt on the CTPV.

Diagnostic evaluations of ultrasound and magnetic resonance imaging in mammary duct ectasia and breast cancer.

The objective of the present study was to evaluate the diagnostic efficiency of ultrasound (US) and magnetic resonance imaging (MRI) in the diagnosis and differential diagnosis of mammary duct ectasia (MDE) and breast cancer. This retrospective study was performed on 35 patients with MDE and 105 patients with breast cancer using US and MRI. Imaging features, semi-quantitative and quantitative parameters were analyzed to determine their diagnostic value for MDE and breast cancer. The average age of patients with breast cancer was increased compared with that of patients with MDE. There were no significant differences in local packages with or without tenderness ratio (P=0.259) and grade of color Doppler flow imaging (P=0.273) between the two groups. However, the morphological changes were significantly increased in breast cancer compared with MDE. In addition, there were significant diagnostic differences in US and MRI between breast cancer and MDE, including resistance index, US elastography, time-signal intensity curve, apparent diffusion coefficient, early-stage enhancement ratio, peak-of-enhancement ratio and Tpeak (P<0.05). However, there were no observable significant diagnostic differences between US, MRI and US with MRI for MDE and breast cancer (P=0.103, P=0.263 and P=0.403 respectively). Diagnosis of MDE and breast cancer requires full evaluation of multiple parameters and morphological changes of US and MRI to increase the diagnostic efficiency. US, MRI and US with MRI were all of diagnostic value for MDE and breast cancer, while US with MRI had the highest efficacy.

Estimation of fetal weight by ultrasonic examination.

PURPOSE: This study was to evaluate the accuracy and clinical application of a new technique in the fetal weight estimation by ultrasound. METHODS: The fetal biparietal diameter (BPD), head circumference (HC) and femur length (FL) were measured by ultrasound examination within one week before delivery. Two different fetal abdominal sectors were measured for the assessment of abdominal circumference (AC). The fetal weight of 516 singleton fetuses was estimated according to above measurements and their actual birth weight was recorded after delivery. RESULTS: There were no significant differences in the fetal AC or body weight evaluated before delivery and recorded after delivery. CONCLUSION: This new technique is more convenient and applicable for the evaluation of fetal AC as compared to standard method, and seems to be reliable and accurate for the assessment of fetal weight.

Elasticity of healthy Achilles tendon decreases with the increase of age as determined by acoustic radiation force impulse imaging.

AIMS: The objective of the study was to investigate the value of acoustic radiation force impulse (ARFI) and its application in Achilles tendon elasticity of healthy volunteers. METHODS: Bilateral Achilles tendons of 56 healthy individuals were included in the study. The subjects were divided into four age groups. Shear wave velocities (SWVs) of each tendon in the states of relaxation and tension were measured using ARFI technology. We selected the middle section of the Achilles tendon for ARFI measurement. Each Achilles tendon was examined by a musculoskeletal radiologist. Longitudinal images of each tendon were obtained by ultrasound and ARFI elastography. A quantitative assessment of the tissue stiffness was made with virtual touch tissue quantification. RESULTS: Significant differences in SWVs of Achilles tendon were found between any two age groups in the same status (P < 0.05) and between different status in subjects aged 25-35 years (P < 0.05) and 36-45 years (P < 0.05). SWV of Achilles tendon increased with increasing age in both states of relaxation and tension, which was consistent with the histological feature of ageing tendons. However, there were no significant differences between men and women within any group in the same status (P > 0.05) or between different states in subjects aged 46-55 years (P = 0.308) and 56-65 years (P = 0.362). CONCLUSIONS: ARFI imaging provides quantitative information about tendon stiffness and represents an excellent supplementary technique to B-mode ultrasound. Furthermore, the elasticity of healthy tendon decreases with increasing age in subjects without disease and long-term heavy load lifting.

Legumain protein as a potential predictive biomarker for Asian patients with breast carcinoma.

BACKGROUND: Treatment for breast cancer is mainly performed by surgical resection of primary tumors and chemotherapy. However, after tumor invasion and metastases, breast cancer is hard to control. Clarification of the pathogenic mechanisms would be helpful to the prognosis or therapy for the breast cancer. The aim of this study is to investigate the clinical and prognostic implications of legumain protein Materials and Methods: In this study, we examined mastectomy specimens from 114 breast cancer and matching, 26 adjacent non-cancerous tissues using immunohistochemistry. RESULTS: The results indicated that positive expression of legumain protein in breast cancer was 51.8 % (59/114) and the positive expression of legumain protein in adjacent non-cancerous tissue was 11.5% (3/26). It appeared to be related with lymph node metastasis of breast cancer (p=0.02) and correlation analysis indicated that legumain expression was correlated positively with the estrogen receptor (ER) and mutant-type p53 expression (both p<0.05). Positive legumain expression was significantly associated with shorter overall survival time in breast cancer patients (log-rank p<0.01). Multivariate survival analysis suggested that the positive legumain expression was an independent predictor of poorer overall survival in patients with breast cancer (HR=0.24; 95%CI 0.11-0.65, p=0.03). CONCLUSIONS: Legumain might be a new potential biomarker for breast cancer, which may reflect the prognosis and overall survival.