Global Research Trends on Monkeypox Virus: A Bibliometric and Visualized Study.

Monkeypox is a zoonotic viral disease that has recently emerged as another global infection disease. A double-stranded enveloped deoxyribonucleic acid virus the cause of this disease. Since monkeypox is an evolving field of study with a growing interest in public health, it is crucial to study the scientific trend and research activities. This study provides an essential insight into the research response to scientific trends of monkeypox using the bibliometric analysis technique. A literature search for published articles on LSD from 2001 to 2021 was conducted in Scopus on 24 July 2022. Visualization analysis was performed using R statistical software. The growth and trend of documents, country-level distribution of publications and collaborations, and the relationship between authors and co-authors were analyzed. Findings revealed a significant increase in the research conducted, mainly from the United States (US). The top 12 institutions published papers on the monkeypox virus, accounting for 33.09 percent of the articles. The US was the most productive nation, producing 275 documents (54.34%), or one-third of all publications in this sector worldwide. Centers for Disease Control and Prevention in Georgia in the United States were the organization that produced the most (365 publications). The Journal of Virology garnered the most citations, with an h-index of 18. In the last year, there has been an increase in the publication of monkeypox virus-related studies. The importance of the monkeypox virus highlights the necessity for continued research to help international health organizations identify areas that require prompt action to implement suitable solutions. This study also provides scaling-up analysis, evidence dissemination on the monkeypox virus, emerging hotspots, and perceptive remarks on the technological advances in this field.

Allele frequency data of 15 autosomal STRs in Arain population of Pakistan.

Fifteen autosomal STRs were evaluated using Identifiler plus kit in 121 Arain samples of Pakistan. The highly discriminatory locus was D2S1338 with value of 0.968. Allele 8 at TPOX was the most frequent with value of 0.467. No significant deviations from Hardy-Weinberg equilibrium were seen except D3S1358 and D18S51. Combined power of discrimination, combined power of exclusion, and combined matching probability were obtained as 0.9999999999999999925, 0.99999815, and 7.4897 × -18, respectively. Population differentiation test demonstrated significant differences between Arain and geographically distinct populations.

Population genetic portrait of Pakistani Lahore-Christians based on 32 STR loci.

Phylogenetic relationship and the population structure of 500 individuals from the Christian community of Lahore, Pakistan, were examined based on 15 autosomal short tandem repeats (STRs) using the AmpFℓSTR Identifiler Plus PCR Amplification Kit and our previously published Y-filer kit data (17 Y-STRs) of same samples. A total of 147 alleles were observed in 15 loci and allele 11 at the TPOX locus was the most frequent with frequency value (0.464). The data revealed that the Christian population has unique genetic characteristics with respect to a few unusual alleles and their frequencies relative to the other Pakistani population. Significant deviations from Hardy-Weinberg equilibrium were found at two loci (D13S317, D18S51) after Boneferroni's correction (p ≤ 0.003). The combined power of discrimination, combined power of exclusion and cumulative probability of matching were 0.999999999999999978430815060354, 0.999995039393942 and 2.15692 × 10-17, respectively. On the bases of genetic distances, PCA, phylogenetic and structure analysis Lahore-Christians appeared genetically more associated to south Asian particularly Indian populations like Tamil, Karnataka, Kerala and Andhra Pradesh than rest of global populations.

Extracellular Matrix and Cellular Plasticity in Musculoskeletal Development.

Cellular plasticity refers to the ability of cell fates to be reprogrammed given the proper signals, allowing for dedifferentiation or transdifferentiation into different cell fates. In vitro, this can be induced through direct activation of gene expression, however this process does not naturally occur in vivo. Instead, the microenvironment consisting of the extracellular matrix (ECM) and signaling factors, directs the signals presented to cells. Often the ECM is involved in regulating both biochemical and mechanical signals. In stem cell populations, this niche is necessary for maintenance and proper function of the stem cell pool. However, recent studies have demonstrated that differentiated or lineage restricted cells can exit their current state and transform into another state under different situations during development and regeneration. This may be achieved through (1) cells responding to a changing niche; (2) cells migrating and encountering a new niche; and (3) formation of a transitional niche followed by restoration of the homeostatic niche to sequentially guide cells along the regenerative process. This review focuses on examples in musculoskeletal biology, with the concept of ECM regulating cells and stem cells in development and regeneration, extending beyond the conventional concept of small population of progenitor cells, but under the right circumstances even "lineage-restricted" or differentiated cells can be reprogrammed to enter into a different fate.

Phylogenetic analysis and haplotype diversity in Christian residents of Lahore, Pakistan, using 17 Y-chromosomal STR loci.

This population data pertains to 250 unrelated male residents of the Christian population of Lahore, Pakistan. AmpF/STR®Yfiler PCR amplification kit was utilized for evaluation of 17 Y-chromosomal STRs loci. Ancestral lineages and parameters of forensic importance were examined leading to recognition of 135 unique out of 175 total haplotypes with diversity value of 0.991. All Y-STRs portrayed high discrimination power where DYS385 depicted the maximum value of 0.917. Multidimensional Scaling (MDS) and Analysis of Molecular Variance (AMOVA) were generated using YHRD (Y-Chromosome STR Haplotype Reference Database) tools. A pair-wise genetic distance comparison using Rst, Fst, and associated p values lead to the conclusion that studied Christian community was significantly demarcated from the rest of the global populations.

Genetic polymorphism of Y-chromosomal STRs in Gujjar population of Punjab.

Y-STR polymorphism of Gujjar population was determined by using AmpFISTR®YfilerTM PCR amplification kit. A total 176 haplotypes were obtained after the analysis of 17 Y-STR loci in 176 genetically unrelated individuals. Haplotype diversity and discrimination capacity attained was 0.99730 and 0.652201325, respectively. The comparison of Gujjar population with 16 other populations revealed that Gujjars have low genetic distance from Punjabi, Sindhi, and Pakhtun population of Pakistan; Azad Kashmir, Saraswat Brahmin from India; Bangladeshi population; north and south of Afghanistan; and Uttar Pradesh India which hints toward the migrational route Gujjars took over the centuries. This data is of significant value for population studies and forensic applications.