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1.
Gastroenterology ; 159(6): 2203-2220.e14, 2020 12.
Article in English | MEDLINE | ID: mdl-32814112

ABSTRACT

BACKGROUND AND AIMS: The pattern of genetic alterations in cancer driver genes in patients with hepatocellular carcinoma (HCC) is highly diverse, which partially explains the low efficacy of available therapies. In spite of this, the existing mouse models only recapitulate a small portion of HCC inter-tumor heterogeneity, limiting the understanding of the disease and the nomination of personalized therapies. Here, we aimed at establishing a novel collection of HCC mouse models that captured human HCC diversity. METHODS: By performing hydrodynamic tail-vein injections, we tested the impact of altering a well-established HCC oncogene (either MYC or ß-catenin) in combination with an additional alteration in one of eleven other genes frequently mutated in HCC. Of the 23 unique pairs of genetic alterations that we interrogated, 9 were able to induce HCC. The established HCC mouse models were characterized at histopathological, immune, and transcriptomic level to identify the unique features of each model. Murine HCC cell lines were generated from each tumor model, characterized transcriptionally, and used to identify specific therapies that were validated in vivo. RESULTS: Cooperation between pairs of driver genes produced HCCs with diverse histopathology, immune microenvironments, transcriptomes, and drug responses. Interestingly, MYC expression levels strongly influenced ß-catenin activity, indicating that inter-tumor heterogeneity emerges not only from specific combinations of genetic alterations but also from the acquisition of expression-dependent phenotypes. CONCLUSIONS: This novel collection of murine HCC models and corresponding cell lines establishes the role of driver genes in diverse contexts and enables mechanistic and translational studies.


Subject(s)
Carcinoma, Hepatocellular/genetics , Genetic Heterogeneity , Proto-Oncogenes/genetics , Animals , Carcinoma, Hepatocellular/immunology , Carcinoma, Hepatocellular/pathology , Cell Line, Tumor , Computational Biology , Disease Models, Animal , Drug Resistance, Neoplasm/genetics , Female , Gene Expression Regulation, Neoplastic/immunology , Humans , Liver Neoplasms/immunology , Liver Neoplasms/pathology , Male , Mice , Mice, Transgenic , Tumor Escape/genetics , Tumor Microenvironment/genetics , Tumor Microenvironment/immunology
2.
Development ; 143(2): 318-28, 2016 Jan 15.
Article in English | MEDLINE | ID: mdl-26657765

ABSTRACT

Lens epithelial cells differentiate into lens fibers (LFs) in response to a fibroblast growth factor (FGF) gradient. This cell fate decision requires the transcription factor Prox1, which has been hypothesized to promote cell cycle exit in differentiating LF cells. However, we find that conditional deletion of Prox1 from mouse lenses results in a failure in LF differentiation despite maintenance of normal cell cycle exit. Instead, RNA-seq demonstrated that Prox1 functions as a global regulator of LF cell gene expression. Intriguingly, Prox1 also controls the expression of fibroblast growth factor receptors (FGFRs) and can bind to their promoters, correlating with decreased downstream signaling through MAPK and AKT in Prox1 mutant lenses. Further, culturing rat lens explants in FGF increased their expression of Prox1, and this was attenuated by the addition of inhibitors of MAPK. Together, these results describe a novel feedback loop required for lens differentiation and morphogenesis, whereby Prox1 and FGFR signaling interact to mediate LF differentiation in response to FGF.


Subject(s)
Homeodomain Proteins/metabolism , Lens, Crystalline/cytology , Lens, Crystalline/metabolism , Receptors, Fibroblast Growth Factor/metabolism , Tumor Suppressor Proteins/metabolism , Animals , Cell Differentiation/drug effects , Cell Differentiation/genetics , Fibroblast Growth Factors/pharmacology , Homeodomain Proteins/genetics , Mice , Mice, Inbred C57BL , Receptors, Fibroblast Growth Factor/genetics , Signal Transduction/drug effects , Signal Transduction/physiology , Tumor Suppressor Proteins/genetics
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