ABSTRACT
We address the enhanced bone growth on designed nanocrystalline zirconia implants as reported by in vivo experiments. In vitro experiments demonstrate that the activation of adhesive proteins on nanoengineered zirconia stimulates cell adhesion and growth as shown by confocal microscopy. Fibrillar fibronectin (FN) forms a matrix assembly on the nanostructured surface in the cell adhesion process. We discuss the importance of FN dimer activation due to its immobilization on the designed nanocrystalline ZrO2 implant fabricated by ion beam assisted deposition. The Monte-Carlo analysis indicates that FN activation on the surface can be promoted by selective electrostatic interactions between negatively charged ZrO2 surface patches and oppositely charged FN domains.
Subject(s)
Cell Adhesion/drug effects , Cell Proliferation/drug effects , Fibronectins/chemistry , Nanotechnology/methods , Zirconium/chemistry , Adsorption , Animals , Biocompatible Materials , Bone Marrow Cells/drug effects , Cell Membrane/metabolism , Computer Simulation , Crystallization , Dimerization , Microscopy, Confocal , Monte Carlo Method , Protein Structure, Tertiary , Rats , Static Electricity , Stromal Cells/drug effects , Surface PropertiesABSTRACT
A novel approach to treating acute leukemias is suggested by hypothesizing a focal unique origin in a focal area of bone marrow and this area can be localized (and ablated) before the leukemia stem cells have seeded other bone marrows.
Subject(s)
Bone Marrow/pathology , Hematopoietic Stem Cells/pathology , Leukemia/therapy , Acute Disease , Humans , Leukemia/etiology , Leukemia/pathologySubject(s)
Factor VIII , Sterilization/methods , Detergents , Freeze Drying , Hot Temperature , Humans , Solvents , VirusesABSTRACT
A case of primary leiomyosarcoma of the abdominal aorta is reported. The tumor replaced the full thickness of the aortic wall with formation of a large saccular aortic aneurysm filled with an admixture of tumor and mural thrombus. Electron microscopy of the surgical biopsy of the tumor revealed neoplastic smooth muscle cells with myofilaments and a discontinuous basal lamina. To our knowledge, leiomyosarcoma of the aorta has never been reported.
