Antiseptic myramistin possesses the strong anti-HIV properties.

The anti-HIV properties of cation detergent myramistin were studied. The dose-dependent slowing down both of the HIV antigens accumulation in supernatants and the virus-dependent cell death was shown at myramistin concentrations 30 micrograms/ml and 50 micrograms/ml in the MT-4 cell line. Simultaneous addition of the trace amount of the detergents and HIV-1 to the cells of Jurkat-tat line did not stimulate the HIV p24 production for 4 days of the experiment.

[Study of the anti-HIV activity of miramistin]. / Izuchenie anti-VICh-aktivnosti miramistina.

The anti-HIV activity of a cationic detergent myramistin and nonionic detergent dezintegron-0 (d-0) was studied using HIV-1 strains III B/H9 and BRU in lymphoblastoid cells MT-4 and Jurkat-tat. Myramistin in a concentration of 0.075 mg/ml was shown to prevent HIV-1 replication in MT-4 when these cells were cocultivated with the cells preinfected and treated with the detergent. Myramistin in concentrations from 0.030 to 0.050 mg/ml delayed the accumulation of virus antigens in the cells by 4 and 14 days, respectively, without preventing the infection of intact cells. Nonionic d-0 prevented HIV-1 infection of intact Jurkat-tat cells at a concentration of 12.5 mg/ml.

[The use of the Soviet detergents dezintegron-O and dezintegron-B for the solubilization of influenza virus glycoproteins]. / Ispol'zovanie otechestvennykh detergentov dezintegrona-O i dezintegrona-B dlia soliubilizatsii glikoproteidov virusa grippa.

Native detergents, desintegron-O-and desintegron-B, solubilize hemagglutinins of the influenza virus as intensively, as foreign drugs (mulgophen, zwittergent and sodium sarcozyl) do. This permits recommending desintegrons for quantitative determination of hemagglutinin of the influenza virus in virus-containing materials by means of the reaction of single radial immunodiffusion. Desintegron-O and desintegron-B permit extracting enzymatically active neuraminidase from influenza A viruses with 44.6-83.4% yield.

[Mutagenic and carcinogenic action of antimicrobial surface-active substances]. / Izuchenie mutagennogo i kantserogennogo deistviia antimikrobnykh poverkhnostno-aktivnykh veshchestv.

The study on the mutagenic activity of commercial synthetic cationic, anionic, amphoteric and nonionogenic surface active substances with antimicrobial properties demonstrated that they had no mutagenic effect on the microbial test systems, i.e. the histidine-deficient strains of Salmonella typhimurium on direct contact and under conditions of metabolic activation in vitro and in vivo. The cationoactive preparation B Kh-14 with the benzyl radical adjoining the nitrogen atom and linear hydrophobic C13H27 with the amide group having a pronounced antimicrobial effect on gram-positive and gram-negative bacteria and fungi showed no carcinogenic activity in the experiments with its long-term action on the urinary bladder mucosa and skin of rats, guinea-pigs and dogs.

[Studies on influence of detergents on human serum albumin using temperature-perturbation difference spectrophotometry]. / Izuchenie vliianiia detergentov na syvorotochnyi al'bumin cheloveka metodom temperaturno-perturbatsionnoi differentsial'noi spektrofotometrii.

The temperature-perturbation difference spectra (TPDS) of human serum albumin (NSA) were studied after incubation with different synthetic detergents--anionic, cationic, amphoteric, nonionogenic--in various molar ratios. It is shown that the detergents studied have a different effect on the perturbation of tyrosine residua spectra. Under these conditions the rigidity of their environment depending on the nature and concentration of the mentioned detergents is supposed to change.