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1.
Int J Immunopathol Pharmacol ; 37: 3946320231185703, 2023.
Article in English | MEDLINE | ID: mdl-37364162

ABSTRACT

OBJECTIVE: SARS-CoV-2 infection has been shown to result in increased circulating levels of adenosine triphosphate and adenosine diphosphate and decreased levels of adenosine, which has important anti-inflammatory activity. The goal of this pilot project was to assess the levels of soluble CD73 and soluble Adenosine Deaminase (ADA) in hospitalized patients with COVID-19 and determine if levels of these molecules are associated with disease severity. METHODS: Plasma from 28 PCR-confirmed hospitalized COVID-19 patients who had varied disease severity based on WHO classification (6 mild/moderate, 10 severe, 12 critical) had concentrations of both soluble CD73 and ADA determined by ELISA. These concentrations were compared to healthy control plasma that is commercially available and was biobanked prior to the start of the pandemic. Additionally, outcomes such as WHO ordinal scale for disease severity, ICU admission, needed for invasive ventilation, hospital length of stay, and development of thrombosis during admission were used as markers of disease severity. RESULTS: Our results show that both CD73 and ADA are decreased during SARS-CoV-2 infection. The level of circulating CD73 is directly correlated to the severity of the disease defined by the need for ICU admission, invasive ventilation, and hospital length of stay. Low level of CD73 is also associated with clinical thrombosis, a severe complication of SARS-CoV-2 infection. CONCLUSION: Our study indicates that adenosine metabolism is down-regulated in patients with COVID-19 and associated with severe infection. Further large-scale studies are warranted to investigate the role of the adenosinergic anti-inflammatory CD73/ADA axis in protection against COVID-19.


Subject(s)
COVID-19 , Humans , Adenosine Deaminase/metabolism , SARS-CoV-2 , Pilot Projects , Adenosine/metabolism , Patient Acuity
2.
Cytometry A ; 103(2): 153-161, 2023 02.
Article in English | MEDLINE | ID: mdl-35246910

ABSTRACT

We evaluated the number of CD26 expressing cells in peripheral blood of patients with COVID-19 within 72 h of admission and on day 4 and day 7 after enrollment. The majority of CD26 expressing cells were presented by CD3+ CD4+ lymphocytes. A low number of CD26 expressing cells were found to be associated with critical-severity COVID-19 disease. Conversely, increasing numbers of CD26 expressing T cells over the first week of standard treatment was associated with good outcomes. Clinically, the number of circulating CD26 cells might be a marker of recovery or the therapeutic efficacy of anti-COVID-19 treatment. New therapies aimed at preserving and increasing the level of CD26 expressing T cells may prove useful in the treatment of COVID-19 disease.


Subject(s)
COVID-19 , Dipeptidyl Peptidase 4 , Humans , Lymphocytes
3.
Sci Rep ; 11(1): 22463, 2021 11 17.
Article in English | MEDLINE | ID: mdl-34789851

ABSTRACT

SARS-CoV-2 infection results in a spectrum of outcomes from no symptoms to widely varying degrees of illness to death. A better understanding of the immune response to SARS-CoV-2 infection and subsequent, often excessive, inflammation may inform treatment decisions and reveal opportunities for therapy. We studied immune cell subpopulations and their associations with clinical parameters in a cohort of 26 patients with COVID-19. Following informed consent, we collected blood samples from hospitalized patients with COVID-19 within 72 h of admission. Flow cytometry was used to analyze white blood cell subpopulations. Plasma levels of cytokines and chemokines were measured using ELISA. Neutrophils undergoing neutrophil extracellular traps (NET) formation were evaluated in blood smears. We examined the immunophenotype of patients with COVID-19 in comparison to that of SARS-CoV-2 negative controls. A novel subset of pro-inflammatory neutrophils expressing a high level of dual endothelin-1 and VEGF signal peptide-activated receptor (DEspR) at the cell surface was found to be associated with elevated circulating CCL23, increased NETosis, and critical-severity COVID-19 illness. The potential to target this subpopulation of neutrophils to reduce secondary tissue damage caused by SARS-CoV-2 infection warrants further investigation.


Subject(s)
COVID-19/immunology , Neutrophils/immunology , Pseudogenes/immunology , Aged , Chemokines/metabolism , Cohort Studies , Critical Illness , Cytokines/metabolism , Enzyme-Linked Immunosorbent Assay/methods , Extracellular Traps/metabolism , Female , Humans , Inflammation/metabolism , Male , Middle Aged , Neutrophils/metabolism , Pseudogenes/genetics , SARS-CoV-2/immunology , SARS-CoV-2/pathogenicity , Severity of Illness Index
5.
J Immunol ; 183(5): 3268-77, 2009 Sep 01.
Article in English | MEDLINE | ID: mdl-19675165

ABSTRACT

Nur77, an orphan nuclear receptor, plays a key role in apoptosis in T cells. In cancer cell lines, Nur77 can induce apoptosis through the intrinsic apoptotic pathway, but the mechanism by which Nur77 kills T cells remains controversial. In this study, we provide biochemical, pharmacological, and genetic evidence demonstrating that Nur77 induces apoptosis through the activation of the intrinsic pathway in T cells. We also show that Nur77 is a physiological substrate of the MEK-ERK-RSK cascade. Specifically, we demonstrate that RSK phosphorylates Nur77 at serine 354 and this modulates Nur77 nuclear export and intracellular translocation during T cell death. Our data reveal that Nur77 phosphorylation and mitochondrial targeting, regulated by RSK, defines a role for the MEK1/2-ERK1/2 cascade in T cell apoptosis.


Subject(s)
Apoptosis/immunology , DNA-Binding Proteins/metabolism , Extracellular Signal-Regulated MAP Kinases/physiology , MAP Kinase Kinase Kinases/physiology , MAP Kinase Signaling System/immunology , Mitochondria/metabolism , Receptors, Steroid/metabolism , Ribosomal Protein S6 Kinases/physiology , T-Lymphocytes/cytology , Active Transport, Cell Nucleus/immunology , Animals , Cell Line , Cells, Cultured , Humans , Intracellular Fluid/enzymology , Intracellular Fluid/immunology , Intracellular Fluid/metabolism , Mice , Mice, Transgenic , Mitochondria/enzymology , Nuclear Receptor Subfamily 4, Group A, Member 1 , Phosphorylation , Protein Transport/immunology , T-Lymphocytes/immunology , T-Lymphocytes/metabolism
6.
J Biol Chem ; 280(22): 21329-36, 2005 Jun 03.
Article in English | MEDLINE | ID: mdl-15799969

ABSTRACT

Upon stimulation by the transforming growth factor beta (TGF-beta), Smad2 and Smad3 are phosphorylated at their C termini and assemble into stable heteromeric complexes with Smad4. These complexes are the functional entities that translocate into the nucleus and regulate the expression of TGF-beta target genes. Here we report that the TGF-beta-activated phospho-Smad3/Smad4 complex utilizes an importin-independent mechanism for nuclear import and engages different nucleoporins for nuclear import compared with the monomeric Smad4. Within the heteromeric complex, phospho-Smad3 appears to dominate over Smad4 in the nuclear import process and guides the complex to its nuclear destination. We also demonstrate that the binding of phospho-Smad3 to Smad4 prevents Smad4 from interacting with the nuclear export receptor chromosome region maintenance 1. In this way, TGF-beta signaling suppresses nuclear export of Smad4 by chromosome region maintenance 1 and thereby targets Smad4 into the nucleus. Indeed tumorigenic mutations in Smad4 that affect its interaction with Smad2 or Smad3 impair nuclear accumulation of Smad4 in response to TGF-beta.


Subject(s)
Cell Nucleus/metabolism , DNA-Binding Proteins/metabolism , Trans-Activators/metabolism , Transforming Growth Factor beta/metabolism , Active Transport, Cell Nucleus , Animals , COS Cells , Cell Nucleolus/metabolism , Cytosol/metabolism , Dimerization , Glutathione Transferase/metabolism , HeLa Cells , Humans , Immunohistochemistry , Immunoprecipitation , Karyopherins/chemistry , Microscopy, Fluorescence , Mutation , Phosphorylation , Protein Binding , Protein Structure, Tertiary , Protein Transport , Recombinant Proteins/chemistry , Signal Transduction , Smad Proteins , Smad3 Protein , Smad4 Protein , Transfection
7.
Proc Natl Acad Sci U S A ; 99(10): 6737-42, 2002 May 14.
Article in English | MEDLINE | ID: mdl-12011435

ABSTRACT

Of the three critical enhancer elements that mediate beta-cell-specific and glucose-responsive expression of the insulin gene, only the identity of the transcription factor binding to the RIPE3b element (RIPE3b1) has remained elusive. Using a biochemical purification approach, we have identified the RIPE3b1 factor as a mammalian homologue of avian MafA/L-Maf (mMafA). The avian MafA is a cell-type determination factor that expressed ectopically can trigger lens differentiation program, but no mammalian homologue of avian MafA has previously been identified. Here, we report cloning of the human mafA (hMafA) and demonstrate that it can specifically bind the insulin enhancer element RIPE3b and activate insulin-gene expression. In addition, mMafA has a very restrictive cellular distribution and is selectively expressed in pancreatic beta but not in alpha cells. We suggest that mMafA has an essential role in the function and differentiation of beta-cells and thus may be associated with the pathophysiological origins of diabetes.


Subject(s)
Homeodomain Proteins , Insulin/genetics , Proto-Oncogene Proteins/metabolism , Trans-Activators/metabolism , Amino Acid Sequence , Animals , Binding Sites , Cricetinae , Crystallins/genetics , Enhancer Elements, Genetic , Gene Expression , HeLa Cells , Humans , Islets of Langerhans , Lectins, C-Type , Maf Transcription Factors, Large , Molecular Sequence Data , Proto-Oncogene Proteins/classification , Receptors, Immunologic , Sequence Homology, Amino Acid , Trans-Activators/classification , Trans-Activators/genetics , Trans-Activators/isolation & purification , Tumor Cells, Cultured
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