ABSTRACT
Proviral integration of a simian T-cell leukemia virus, S(H)TLV-I, highly homologous to human T-cell leukemia virus type 1 was examined in cellular DNAs isolated from lymphoid organs of lymphomatous and healthy baboons from the Sukhumi monkey colony. Most of the sick and some of healthy monkeys contained HTLV-I-related sequences in their chromosomal DNAs. Judging from the integration site of the provirus genome, S(H)TLV-I-infected cells had selective preferences and proliferated monoclonally.
Subject(s)
Cloning, Molecular , Lymphoma/genetics , Monkey Diseases/genetics , Papio/microbiology , Simian T-lymphotropic virus 1/genetics , Animals , Base Sequence/genetics , DNA/genetics , DNA, Viral/genetics , Genes, Viral/genetics , Lymphoma/microbiology , Lymphoma/veterinary , Molecular Sequence Data , Monkey Diseases/microbiology , Nucleic Acid Hybridization , Proviruses/geneticsABSTRACT
The association of simian T-cell leukemia virus with baboons malignant lymphoma was studied. Proviral DNA integrated in cells of lymphoid organs was detected in 19 of 21 sick animals examined. Only 4 of 10 monkeys from high risk colony without clinical manifestations had virus-specific sequences in their cellular DNAs. As a rule, S(H)TLV-I-infected cells monoclonally proliferated both in lymphomatous and healthy baboons. The restriction patterns with EcoRI, HindIII, PstI, BamHI of S(H)TLV-I provirus were similar but not identical to those of prototype HTLV-I and STLV-I isolates.
