Effects of Gold Nanoparticles on Mentha spicata L., Soil Microbiota, and Human Health Risks: Impact of Exposure Routes.

Nanoparticles, due to their extensive production and application, can have significant consequences for the environment, including soil and plant pollution. Therefore, it is very important to assess how nanoparticles will affect plants depending on the exposure pathways. The effect of gold nanoparticles in a concentration range of 1-100 mg/L on Mentha spicata L. during a 28-day experiment was investigated. Two routes of nanoparticles exposure were applied: root and foliar. Transmission electron microscopy was used to characterize nanoparticles and their effect on plant leaves' ultrastructure. Gold content in soil and plant segments was determined using k0-neutron activation analysis. For root exposure, gold was mainly accumulated in soil (15.2-1769 mg/kg) followed by root systems (2.99-454 mg/kg). The maximum accumulation of gold in leaves (5.49 mg/kg) was attained at a nanoparticle concentration of 100 mg/L. Foliar exposure resulted in the maximum uptake of gold in leaves (552 mg/kg) and stems (18.4 mg/kg) at the highest applied nanoparticle concentration. The effect of nanoparticles on the Mentha spicata L. leaves' biochemical composition was assessed. Nanoparticles affected the content of chlorophyll and carotenoids and led to an increase in antioxidant activity. Root exposure to gold nanoparticles resulted in an increase in the number of starch grains in chloroplasts and also suppressed the activity of the soil microbiota. Gold extraction from mint leaves into herbal infusion varied from 2 to 90% depending on the concentration of nanoparticles in the solution and the exposure route. The health risk as a result of gold exposure via herbal tea intake was assessed through estimated daily intake. The hazard quotient values were found to be less than the cutoff, indicating that a cup of tea infusion should not cause a serious impact to human health.

Effects of Silver Nanoparticles on the Red Microalga Porphyridium purpureum CNMN-AR-02, Cultivated on Two Nutrient Media.

The purpose of this study was to examine the influence of 10 and 20 nm nanoparticles (AgNPs) on the growth and biochemical composition of microalga Porphyridium purpureum CNMN-AR-02 in two media which differ by the total amount of mineral salts (MM1 with 33.02 g/L and MM2 with 21.65 g/L). Spectrophotometric methods were used to estimate the amount of biomass and its biochemical composition. This study provides evidence of both stimulatory and inhibitory effects of AgNPs on different parameters depending on the concentration, size, and composition of the nutrient medium. In relation to the mineral medium, AgNPs exhibited various effects on the content of proteins (an increase up to 20.5% in MM2 and a decrease up to 36.8% in MM1), carbohydrates (a decrease up to 35.8% in MM1 and 39.6% in MM2), phycobiliproteins (an increase up to 15.7% in MM2 and 56.8% in MM1), lipids (an increase up to 197% in MM1 and no changes found in MM2), antioxidant activity (a decrease in both media). The composition of the cultivation medium has been revealed as one of the factors influencing the involvement of nanoparticles in the biosynthetic activity of microalgae.

Evaluation of Holmium(III), Erbium(III), and Gadolinium(III) Accumulation by Cyanobacteria Arthrospira platensis Using Neutron Activation Analysis and Elements' Effects on Biomass Quantity and Biochemical Composition.

Rare-earth elements are released into the aquatic environment as a result of their extensive use in industry and agriculture, and they can be harmful for living organisms. The effects of holmium(III), erbium(III), and gadolinium(III) when added to a growth medium in concentrations ranging from 10 to 30 mg/L on the accumulation ability and biochemical composition of Arthrospira platensis were studied. According to the results of a neutron activation analysis, the uptake of elements by cyanobacteria occurred in a dose-dependent manner. The addition of gadolinium(III) to the growth medium did not significantly affect the amount of biomass, whereas erbium(III) and holmium(III) reduced it up to 22% compared to the control. The effects of rare-earth elements on the content of proteins, carbohydrates, phycobiliproteins, lipids, ß carotene, and chlorophyll a were evaluated. The studied elements had different effects on the primary biomolecule content, suggesting that holmium(III) and erbium(III) were more toxic than Gd(III) for Arthrospira platensis.

Effects of citrate-stabilized gold and silver nanoparticles on some safety parameters of Porphyridium cruentum biomass.

Introduction: Our research raises the question of how realistic and safe it is to use gold and silver nanoparticles in biotechnologies to grow microalgae, which will later be used to obtain valuable products. To this purpose, it was necessary to assess the influence of 10 and 20 nm Au and Ag nanoparticles stabilized in citrate on the growth of microalga Porphyridium cruentum in a closed cultivation system, as well as some safety parameters of biomass quality obtained under experimental conditions. Methods: Two types of experiments were conducted with the addition of nanoparticles on the first day and the fifth day of the cultivation cycle. Changes in productivity, lipid content, malondialdehyde (MDA), as well as antioxidant activity of microalgae biomass have been monitored in dynamics during the life cycle in a closed culture system. Results: The impact of nanoparticles on the growth curve of microalgae culture was marked by delaying the onset of the exponential growth phase. A significant increase in the content of lipids and MDA in biomass was noted. Excessive accumulation of lipid oxidation products within the first 24 h of cultivation resulted in altered antioxidant activity of red algae extracts. Discussion: Citrate-stabilized gold and silver nanoparticles proved to be a stress factor for red microalga Porphyridium cruentum, causing significant changes in both biotechnological and biomass safety parameters. Addition of Au and Ag nanoparticles during the exponential growth phase of porphyridium culture led to an enhanced lipid accumulation and reduced MDA values in biomass.

Features of Copper and Gold Nanoparticle Translocation in Petroselinum crispum Segments.

The application of metal nanoparticles in industry and medicine results in their release into the environment, which can have a negative impact on human health. The effects of gold (AuNPs) and copper (CuNPs) nanoparticles at the concentration range of 1-200 mg/L on parsley (Petroselinum crispum) under conditions of root exposure and their translocation in roots and leaves were investigated in a 10-day experiment. The content of copper and gold in soil and plant segments was determined using ICP-OES and ICP-MS techniques, while the morphology of nanoparticles was analyzed using transmission electron microscopy. Differences in the nanoparticle uptake and translocation were observed: CuNPs mainly accumulated in soil (4.4-465 mg/kg), while accumulation in the leaves were at the control level. AuNPs mainly accumulated in soil (0.04-108 mg/kg), followed by roots (0.05-45 mg/kg) and leaves (0.16-53 mg/kg). The influence of AuNPs and CuNPs on the biochemical parameters of parsley was on the content of carotenoids, the levels of chlorophyll, and antioxidant activity. Application of CuNPs even at the lowest concentration led to a significant reduction in carotenoids and total chlorophyll content. AuNPs at low concentrations promoted an increase in the content of carotenoids; however, they also significantly reduced it at concentrations higher than 10 mg/L. To our knowledge, this is the first study of the effect of metal nanoparticles on parsley.

Modification of Some Structural and Functional Parameters of Living Culture of Arthrospira platensis as the Result of Selenium Nanoparticle Biosynthesis.

Selenium nanoparticles are attracting the attention of researchers due to their multiple applications, including medicine. The biosynthesis of selenium nanoparticles has become particularly important due to the environmentally friendly character of the process and special properties of the obtained particles. The possibility of performing the biosynthesis of selenium nanoparticles via the living culture of Arthrospira platensis starting from sodium selenite was studied. The bioaccumulation capacity of the culture, along with changes in the main biochemical parameters of the biomass, the ultrastructural changes in the cells during biosynthesis and the change in the expression of some genes involved in stress response reactions were determined. Protein, lipid and polysaccharide fractions were obtained from the biomass grown in the presence of sodium selenite. The formation of selenium nanoparticles in the protein fraction was demonstrated. Thus, Arthrospira platensis culture can be considered a suitable matrix for the biosynthesis of selenium nanoparticles.

Arthrospira platensis as Bioremediator of Rhenium Mono- and Polymetallic Synthetic Effluents.

Rhenium is a scarce and highly important metal for industry and technology. In the present study, the cyanobacterium Arthrospira platensis (Spirulina) was used to remove rhenium and related elements (Mo and Cu) from mono- and polymetallic synthetic effluents. Metal ions in different concentrations were added to the culture medium on the first, third, and fifth days of biomass growth, and their uptake by the biomass was traced using ICP-AES technique. The accumulation of rhenium in the biomass was dependent on the chemical composition of the effluents, and the highest uptake of 161 mg/kg was achieved in the Re-Cu system. The presence of rhenium, copper, and molybdenum affected the productivity of Spirulina biomass and its biochemical composition (proteins, carbohydrates, lipids, phycobiliproteins, the content of chlorophyll α and ß-carotene). With the growth of biomass in the presence of rhenium or rhenium and molybdenum, a pronounced increase in productivity and protein content was observed. The presence of copper in systems has a negative effect on biomass productivity and biochemical composition. Arthrospira platensis may be of interest as a bioremediator of rhenium-containing effluents of various chemical compositions.

Application of Cyanobacteria Arthospira platensis for Bioremediation of Erbium-Contaminated Wastewater.

Erbium belongs to rare earth elements critical for industry, especially nuclear technology. Cyanobacteria Arthospira platensis was used for Er(III) removal from wastewater by applying biosorption and bioaccumulation processes. The influence of pH, Er(III) concentration, contact time and temperature on the biosorption capacity of Arthospira platensis was determined. The optimal conditions for Er(III) removal were defined as pH 3.0, time 15 min and temperature 20 °C, when 30 mg/g of Er(III) were removed. The kinetics of the process was better described by the pseudo-first-order model, while equilibrium fitted to the Freundlich model. In bioaccumulation experiments, the uptake capacity of biomass and Er(III) effect on biomass biochemical composition were assessed. It was shown that Er(III) in concentrations 10-30 mg/L did not affect the content of biomass, proteins, carbohydrate and photosynthetic pigments. Its toxicity was expressed by the reduction of the lipids content and growth of the level of malonic dialdehyde. Biomass accumulated 45-78% of Eu(III) present in the cultivation medium. Therefore, Arthospira platensis can be considered as a safe and efficient bioremediator of erbium contaminated environment.

Assessment of Metal Accumulation by Arthrospira platensis and Its Adaptation to Iterative Action of Nickel Mono- and Polymetallic Synthetic Effluents.

Cyanobacteria-mediated wastewater remediation is an economical, efficient, and eco-friendly technology. The present work deals with the bioaccumulation performance of Arthrospira platensis (Spirulina) grown for four cycles in a medium containing nickel mono- and polymetallic synthetic effluents. The metal uptake by spirulina biomass was evaluated using neutron activation analysis. The effects of effluents on biomass production, protein, and phycobiliprotein content were assessed. Metal accumulation in the biomass depended on the effluent composition and metal ion concentrations. Nickel accumulation in the biomass was directly proportional to its concentration in effluents, and maximum uptake (1310 mg/kg) was attained in the Ni/Cr/Fe system. In the same system, biomass accumulated 110 times more chromium and 4.7 times more iron than control. The highest accumulation of copper (2870 mg/kg) was achieved in the Ni/Cu/Zn/Mo system and zinc (1860 mg/kg)-in the Ni/Cu/Zn/Sr system. In biomass grown in the media loaded with nickel and also chromium, iron, copper, strontium, zinc, and molybdenum, a decrease in productivity (on average by 10%) during the first cycle of cultivation and moderate reduction of protein content (by 15-27%) was observed. The presence of metals in the cultivation media inhibited phycobiliprotein synthesis, especially of phycocyanin, and promoted the synthesis of allophycocyanin. The maximum reduction of phycocyanin content was 77%, and the increase of allophycocyanin content-by 45%. Arthrospira platensis may be deemed as bioremediation of nickel-polluted wastewaters of complex composition.

Peculiarities of the Edaphic Cyanobacterium Nostoc linckia Culture Response and Heavy Metal Accumulation from Copper-Containing Multimetal Systems.

Soil and water pollution is a major problem that has a negative impact on ecosystems and human health in particular. In the bioremediation processes, the application of photosynthetic microorganisms, including cyanobacteria, is a direction of action addressed with increasing frequency in the context of further development and improvement of environmentally friendly techniques needed for detoxification of soils and waters polluted with low concentrations of toxic elements, since they pose a challenge for traditional treatment methods. In the present study, the removal of copper and other metal ions from multielement systems by three generations of Nostoc linckia is discussed. Changes in the biochemical composition of the nostoc biomass, which accumulates metal ions, were monitored. Neutron activation analysis was applied to assess Cu, Fe, Ni, and Zn accumulation by biomass, as well as to determine the biochemical composition of biomass after specific biochemical methods were used. The capacity of the accumulation of copper and other metal ions from multi-elemental systems by cyanobacteria Nostoc linckia was high and increased over two cycles of biomass growth in the systems Cu-Fe-Ni and Cu-Fe-Zn and over three cycles in Cu-Fe and Cu-Fe-Ni-Zn systems. It constituted 1720-10,600 µg metal/g depending on the system and cycle of cultivation. The accumulation of Fe, Ni, and Zn also increased over the generations of nostoc. The process of metal accumulation was demonstrated by a significant change in the biomass biochemical composition. Cyanobacteria Nostoc linckia possess a pronounced capacity of copper and other metal ion accumulation from multimetal systems and showed an increased resistance in environments polluted with heavy metals.

Accumulation and Effect of Silver Nanoparticles Functionalized with Spirulina platensis on Rats.

The effect of unmodified and functionalized Spirulina platensis biomass silver nanoparticles on rats during prolonged oral administration was assessed. Silver nanoparticles were characterized by using transmission electron microscopy, while their uptake by the biomass was confirmed using scanning electron microscopy and energy dispersive analysis. The content of silver in the different organs of rats after a period of administration (28 days) or after an additional clearance period (28 days) was ascertained by using neutron activation analysis. In animals administrated with the unmodified nanoparticles, the highest content of silver was determined in the brain and kidneys, while in animals administrated with AgNP-Spirulina, silver was mainly accumulated in the brain and testicles. After the clearance period, silver was excreted rapidly from the spleen and kidneys; however, the excretion from the brain was very low, regardless of the type of nanoparticles. Hematological and biochemical tests were performed in order to reveal the effect of nanoparticles on rats. The difference in the content of eosinophils in the experimental and control groups was statistically significant. The hematological indices of the rats did not change significantly under the action of the silver nanoparticles except for the content of reticulocytes and eosinophils, which increased significantly. Changes in the biochemical parameters did not exceed the limits of normal values. Silver nanoparticles with the sizes of 8-20 nm can penetrate the blood-brain barrier, and their persistence after a period of clearance indicated the irreversibility of this process.

Effect of zinc-containing systems on Spirulina platensis bioaccumulation capacity and biochemical composition.

Cyanobacteria Spirulina platensis due to its high biosorption and bioaccumulation capacity toward metal ions can be considered as an excellent candidate for environmental bioremediation. The effect of Zn and in different combinations on the accumulation capacity of Spirulina platensis biomass and its biochemical composition was investigated. Four Zn-containing systems with a different combination of metal ions (Zn; Zn/Cu/Sr; Zn/Cu/Ni; Zn/Cu/Sr/Ba) and different metal concentrations were modeled. Studied systems were introduced in the cultivation medium on the fifth day of biomass grow and experiments were performed in three variants, which differed by metal ions concentrations. Metal uptake by biomass was traced using neutron activation analysis. Spirulina platensis showed a high accumulation capacity for all metal ions present in the analyzed system. Because the metals were added at the beginning of the stationary growth phase, the contact with the biomass was only 24 h, even at the highest metal concentration in the systems, the accumulation of Spirulina platensis biomass was reduced by no more than 11.2%. Spirulina platensis biomass grown in a mono-metallic system expressed two biochemical indicators of stress: decrease of phycobiliprotein content and increase of malondialdehyde content. In biomass grown in the presence of Zn-containing multi-metallic systems, three indicators of stress were expressed: decrease of protein content, reduction of phycobiliprotein content, and increase of malondialdehyde content. Spirulina platensis biomass can be considered as an effective accumulator for the treatment of zinc-containing industrial effluents.

Biochemical changes in microalga Porphyridium cruentum associated with silver nanoparticles biosynthesis.

For the first time, the microalga Porphyridium cruentum was tested for its ability to produce silver nanoparticles. To characterize formed silver nanoparticles UV-vis Spectrometry, Scanning Electron Microscopy, Energy-dispersive analysis of X-rays and X-ray diffraction were used. It was shown that after biomass exposure to silver nitrate solution the extracellular formation of spherical-like nanoparticles took place. Functional groups responsible for metal binding were determined by Fourier-transform infrared spectroscopy. The complex of biochemical tests was used for biomass characterization and assessment of the changes of its main components (proteins, lipids, carbohydrates, and phycobilin) during nanoparticle formation. Obtained data indicate a significant decrease of proteins, carbohydrates, phycobiliproteins, and lipids content as well as antiradical activity of biomass. The obtained results show the necessity of determination of optimal conditions for obtaining Porphyridium cruentum biomass enriched with silver nanoparticles for its further application in the pharmaceuticals industry.

Spirulina platensis as renewable accumulator for heavy metals accumulation from multi-element synthetic effluents.

Metal accumulation by Spirulina platensis from synthetic effluents with the following chemical composition: Cr/Fe, Cr/Fe/Ni, Cr/Fe/Ni/Zn, and Cr/Fe/Ni/Zn/Cu during repeated cultivation cycle was investigated. Metal ions in different concentrations were added to the culture medium at the exponential and stationary phases of biomass growth and their uptake by biomass was traced using neutron activation analysis. The effect of metal ions on biomass and main biochemical components (proteins, carbohydrates, lipids, phycobilins, and ß-carotene) was monitored. S. platensis keeps high metal accumulation capacity during 2-3 cultivation cycles, while the metal ions were added in the stationary phase of its growth. By adding metals in the exponential phase of growth in the following concentrations: 10 mg/L of chromium (VI), 5 mg/L of iron, 2 mg/L of zinc, nickel, and copper, Spirulina platensis acted as renewable accumulator only in Cr/Fe system. It maintained the accumulation capacity during three cultivation cycles when exposed to lower concentrations of metal ions. Its ability to accumulate metal ions during several cultivation cycles was ensured by the maintenance of the optimal level of proteins and lipid in biomass.

Current Research and New Perspectives in Antifungal Drug Development.

In recent times, fungi are becoming more and more active as causal agents of human infections, which is primarily determined by the growing number of people with severe immunosuppression. Thus, the problems of elucidating the mechanisms of action of antifungal preparations, highlighting ways to obtain resistance to their action and research strategies aimed at discovering new compounds with antifungal properties remain the focus of contemporary biomedicine and pharmaceutics. This paper reviews the recent achievements in antifungal drug development and focuses on new natural antifungal remedies with a noticeable effect on pathogens with minimal adverse effects on the host organism.

Development of several hybrid drying methods used to obtain red beetroot powder.

Reduction of bioactive compounds sensitive to heat, light and oxygen could be attributed to drying. The effectiveness of microwave pretreatment at 525 or 420 W and the ohmic heating at 17.5 V/cm over the properties of beetroot powder were compared. This study aimed to develop novel hybrid drying methods. The effects of microwave and ohmic heating pre-treatment and subsequent microwave-convection drying were studied. Several investigations were performed, such as phenolic content, antioxidant activity, betalains content and color. The samples pretreated by microwave have registered higher polyphenols content and antioxidant capacity than those ohmically heated. The ohmic heating pretreated samples registered highest values for ΔL = 7.33-7.65 and ΔH = 8.18-10.02. Confocal microscopy reveals that the MW heating favors cellular lysis and cell content agglutinates. In conclusion, even if the MW preheating has certain disadvantages, in some cases it provides increased bioavailability generated by the vegetal cell disruption and the release of the bioactive compounds.

Influence of different drying methods on the physicochemical properties of red beetroot (Beta vulgaris L. var. Cylindra).

There is an increased interest in preserving fruits and vegetables by drying. The novelty of this study consists in the combination of the following three drying methods: free convection (at 50, 60, and 70°C), forced convection at 40°C and 315W microwave power. The aim of this study was to investigate the effects of the drying conditions on red beetroot (Beta vulgaris L.) in terms of betalain variance, and polyphenol, microstructure changes (SEM). A strong thermal shock, provided by convection at 60° followed by microwave wattage 315W/9min, leads to a better preservation of bioactive compounds content (0.631±0.0042mg/g of betacyanin and 0.795±0.0019mg/g betaxanthin) when compared to convection at 50, 60 and 70°C. The results showed that combined drying methods led to a significant preservation of the phytochemical content as compared to the traditional methods.