[The use of monoclonal antibodies for detecting the common antigenic determinants of Brucella spp. and Yersinia enterocolitica O:9]. / Ispol'zovanie monoklonal'nykh antitel dlia vyiavleniia obshchikh antigennykh determinant Brucella spp. i Yersinia enterocolitica O:9.

Monoclonal antibodies (McAb) 2AH10, specifically reacting with protein preparations having mol. wt. of 18 and 38 kD and not interacting with brucellar lipopolysaccharide (LPS) and protein-polysaccharide antigen, have been obtained. As shown with the use of McAb 2AH10, Brucella spp. and Yersinia enterocolitica O:9 possess common antigenic determinants, localized not only in the area of their LPS, which is generally known, but also in the area of their outer cell-wall proteins with mol. wt. 18 and 38 kD. The sensitivity of the solid-phase enzyme immunoassay and the latex agglutination test with the use of McAb 2AH10 is essentially higher in the detection of B. abortus and B. suis, than B. melitensis and B. rangiferi, as well as Y. enterocolitica O:9. Essential differences observed in the detected concentrations of different Brucella species and Y. enterocolitica O:9 are seemingly linked with different expression of specific antigenic determinants, detected with the use of McAb 2AH10 in the corpuscular antigens under study.

[The morphological characteristics of globulin production during the vaccinal process induced by tularemia and brucellosis vaccines]. / Morfologicheskaia kharakteristika globulinproduktsii pri vaktsinal'nom protsesse, vyzvannom tuliaremiinoi i brutselleznoi vaktsinami.

Significant data on the dynamics of globulin production in guinea pigs in the process of immunogenesis after the injection of Francisella tularensis vaccine strain or conjugated brucellosis vaccine have been obtained by means of immunofluorescence and the enzyme immunoassay. The number of globulin-producing cells in lymphoid organs (the spleen, regional and remote lymph nodes) differs, depending on the injected antigen. The relationship between the character of immunomorphological changes in lymphoid organs and the dynamics of the increase of antibody titers in the peripheral blood of the animals after their immunization with conjugated brucellosis vaccine and the injection of avirulent F. tularensis has been established.

[The results of using an immunoenzyme method in an epizootiologic survey of natural Siberian foci of plague]. / Itogi primeneniia immunofermentnogo metoda pri épizootologicheskom obsledovanii sibirskikh prirodnykh ochagov chumy.

The approbation of the enzyme immunoassay in the study of diverse field material (corpses of animals, ectoparasites, soil, feces of birds of prey, etc.) for the presence of Yersinia pestis contamination has shown the advantages of this assay over the existing serological tests: the passive hemagglutination test and the antigen (or antibody) neutralization test. The use of the immunoperoxidase preparation obtained on the basis of staphylococcal protein A makes it possible to detect antibodies to Y. pestis in animals of various species known to be the main carriers, as well as less important ones, in the natural foci of plague in Siberia. The enzyme immunoassay is recommended for use in the study of not only active natural foci, but also territories, dangerous with respect to plague, as well as for controlling the state of such territories after the realization of relevant sanitation measures.

[Detection of autoantibodies to blood coagulation factors by the passive hemagglutination reaction and by an immunoenzyme (ELISA) method]. / Vyiavlenie autoantitel k faktoram svertyvaniia krovi reaktsiei passivnoi gemaggliutinatsii i immunofermentnym (ELISA) metodom.

Plasma of healthy persons and intact animals contains antibodies to blood coagulation factors IIa and Xa of autologous origin demonstrated by passive hemagglutination and ELISA. During fibrin formation, autoantibodies are bound and thus are not detectable in the serum. The importance of the facts described for hemostasis control is discussed.